Appendix 2: PCR primer combinations for PCR with toe pad samples from museum specimens.
Primer Sequence bp
cytb complete, longer fragments, younger samples L14841
149 Emb1- GARGCTCCRTTTGCRTGKA
Emb2+ GCCATACATTACACAGCAGA
200 Emb3- ACGTAGCCTACGAAGGCGGTTG
Emb4+ AWAGAAACCTGAAACATCGG
170 Emb5- CGAAAAATCGAGTTAGTGTGG
Emb6+ ATYACAAACCTATTCTCRGC
203 Emb7- TTGATGGTGTAGTATGGGTGGA
Emb8+ GGCCTCACCCTCGTCCACCTCACCTT
204 H-15547 AATAGGAAGTATCATTCGGGTTTGATG
Emb9+ TMGTCTCACTAGCCCTATTCTCCCC
197 Emb10- AGGGGTCGGAAGGTTATGGATC
Emb11+ ACAAACTAGGAGGAGTACTAGC
188 Emb12- ARRATGATTGTGAARTACGHRA
Emb13+ TCTGAGCCCTAGTAGCCAACAT
173 H-16065 AGTCTTCAATCTTTGGCTTACAAGAC
cytb first half, shorter fragments, older samples EmbA+ TGCYTAATYACYCAAATCRTCAC
115 EmbA- CARATRAAGAARAARGARGCTCC
EmbB+ GGCTGACTYATCCGYAA
157 EmbB- GAYATTTGKCCTCAKGG
EmbC+ AAAGARACCTGAAACATYGG
140 EmbC- GARAAYCCRCCTCARGCYC
EmbD+ TYACAAAYCTATTCTCRGCC
144 EmbD- TTTGARCCYGTTTCGTG
Emb8+ GGCCTCACCCTCGTCCACCTCACCTT
140 E- TCTGGGTCNCCTAGKAGGTTDGGGG
F+ ACTAYACYATCAARGACRYCCTRGG
108 H-15547 AATAGGAAGTATCATTCGGGTTTGATG