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PCR-Based Identification of Bacillus thuringiensis Isolated from Soil Samples in Nigeria

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PCR-Based Identification of Bacillus thuringiensis Isolated from Soil Samples in Nigeria

Abiodun A. Ogunjimi

a,b

*, George O. Gbenle

a

, Daniel K. Olukoya

b

and Ezekiel O. Akinrimisi

a

a Department of Biochemistry, College of Medicine of the University of Lagos, P. M. B. 12003, Lagos, Nigeria

b Genetics Division, Nigerian Institute of Medical Research, P. M. B. 2013, Yaba, Lagos, Nigeria

* Author for correspondence and reprint requests

Z. Naturforsch.55 c,987Ð990 (2000); received March1/July 12, 2000

Polymerase Chain Reaction (PCR),Bacillus thuringiensis, Mosquito Larvicidal Activity Six isolates of Bacillus thuringiensisisolated from soil samples confirmed to be toxic to mosquito larvae were differentiated using a PCR-Based technique. Three of these isolates initially identified using a serological technique were further differentiated with the PCR amplification of theδ-endotoxin target sequences. Using the total DNA of isolates as tem- plate, at least four isolates yielded amplicons one or all the crystal protein genes, cryI a, b, c, or II withsizes ranging from 238Ð1070 bp. None of these isolates yielded an amplicon for any of Cry IV A, B and D tested. Of the four isolates identified by PCR technique one isolate remained unidentified by serology.

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