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Tri-ReagentNucleospinEIP4-RdRP CDE AB

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(1)

A B

0 10 20 30 40 50 60 70 80 90

1 2

P o sitiv e d ro p lets (n)

Tri-Reagent Nucleospin

E

IP4-RdRP

C D

E

(2)

Kit: iScript

Added primers: none

Kit: iScript

Added primers: E/IP4

Kit: GoTaq random Added primers: E/IP4

Kit: SuperScript III Added primers: E/IP4

Kit: iScript Advanced Added primers: none

Kit: iScript Advanced Added primers: E/IP4

iScript iScript +E/IP4 GoTaq random +E/IP4 SSIII random +E/IP4 iScript Advanced iScript Advanced+E/IP4

E IP4

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Cp d /d ro p let

(3)

0 5 10 15 20 25 30 35 40

CCHF23 Ev-2704 F56AM Ju-2704 M25PPA M57PPa Pa-2704 PELF22 PPAM57

Cq

RACK1

hGAPDH

HPRT1

RPP30

(4)

2 0 0 3 5 1 2 0 0 3 5 3 2 0 0 3 5 7 200323 2 0 0 6 0 0

Bio-Rad in-house

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