Supplementary Information
Table S1 Histological score for lung inflammation
Score Peribronchial infiltrate Alveolar involvement
0 None None
1 Low
(infiltrate ≤ 4 cells thick)
Low
(<25% examined lung with increased cellularity/thickening)
2 Medium
(infiltrate 5‒10 cells thick)
Medium
(25‒50% examined lung with increased cellularity/thickening)
3 High
(25‒50% visualized lumen)
High
(>50% examined lung with increased cellularity/thickening)
4 Diffuse(>50% visualized lumen)
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Table S2 Effect of the FEMP on the serum GOT and GPT levels and body weight in a CA- induced guinea pig model (n = 8/group)
Nor Model FEMP50 FEMP100
Body weight (g) 406.7 ± 3.5 406 ± 4.5 414.4 ± 5.5 413.8 ± 3.4 GOT (U/L) 56.3 ± 12.2 62.2 ± 2.9 63.5 ± 9.7 72.2 ± 14.0
GPT (U/L) 40.17 ± 2.7 43.8 ± 2.8 44.3 ± 2.5 45.3 ± 2.8
Nor, normal control; Model, CA-induced cough control; FEMP50, CA-induced guinea pigs treated with 50 mg/kg/day FEMP; FEMP100, CA-induced guinea pigs treated with 100 mg/kg/day FEMP. The data are expressed as the mean ± SEM
Figure S1 HPLC Chromatogram of the Standards of Oxyresveratrol (Oxy) (A) and Rosmarinic Acid (Ros) (B), and the Active Ingredients of FEMP (C).
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Figure S2 Scheme of OVA/LPS-induced asthma mice study.
Figure S3 Scheme of citric acid induced coughing guinea pigs study.
Figure S4 Viability of FEMP-treated Cells. A549 cells were treated with 2.5, 5 and 10
μg/mL FEMP for 24 h, and the cell viability was measured by the MTT assay. The data are shown as the mean ± SD from three independent experiments.
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