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** ******************* *** ***************** ************ ****** ****** ** Buspirone-HClTime (min)12345-1001020304050 Buspirone-HClTime (min)12345020406080 Buspirone-HClTime (min)12345020406080 Buspirone-HClTime (min)123450100200300400500

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Online Resource 6 Effect of buspirone on zebrafish swimming behavior

(a) Time spent at the top, (b) time spent at the bottom, (c) total distance travelled, and (d) freezing time during the NTT are presented for buspirone (10, 30, and 100 mg/L). Black dashed lines = control; light blue = lowest concentration; blue = middle concentration; purple = highest concentration. For anatabine only, red is the highest concentration. *p<0.05; ***p<0.001. Data are expressed as mean ±SEM.

Top Duration Buspirone-HCl

Time (min)

Time (sec)

1 2 3 4 5

0 20 40 60 80

*** *** *** ***

ControlBuspirone-HCl 10 mg/L Buspirone-HCl 30 mg/L Buspirone-HCl 100 mg/L

*** ** ** ** *** ***

**

Buspirone-HCl

Time (min)

Time (sec)

1 2 3 4 5

0 20 40 60 80

***

***

Control

Buspirone-HCl 10 mg/L Buspirone-HCl 30 mg/L Buspirone-HCl 100 mg/L

**

***

*** * *** ***

*** *

Bottom Duration

Buspirone-HCl

Time (min)

Distance (cm)

1 2 3 4 5

0 100 200 300 400

500

**

Control

Buspirone-HCl 30 mg/L Buspirone-HCl 100 mg/L Buspirone-HCl 10 mg/L

** **

**

Distance Travelled Buspirone-HCl

Time (min)

Time (sec)

1 2 3 4 5

-10 0 10 20 30 40 50

***

ControlBuspirone-HCl 10 mg/L

Buspirone-HCl 30 mg/L Buspirone-HCl 100 mg/L

* *

Freezing Time

a b

c d

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