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(1)

The Augment of Regulatory T Cells Undermines the Efficacy of Anti-PD-L1 treatment in Cervical Cancer

Fengying Xu

1, †

, Fengying Zhang

1, †

, Qian Wang

2

, Ying Xu

3

, Shuifang Xu

1

, Caihong Zhang

1

, Lihua Wang

4*

1. Department of Gynaecology and Obstetrics, Jinshan District Tinglin Hospital, Shanghai, 100191, China.

2. Department of Pathology, Jinshan District Tinglin Hospital, Shanghai, 100191, China.

3. Department of Gastroenterology, Jinshan District Tinglin Hospital, Shanghai. Shanghai, 100191, China.

4. International Peace Maternity & Child Health Hospital, Shanghai JiaoTong University School of Medicine Department of Gynecologic Oncology, Shanghai, 200025, China.

*Correspondence author: Li-hua Wang, International Peace Maternity & Child Health Hospital, Shanghai JiaoTong University School of Medicine Department of Gynecologic Oncology. Address: 910 Hengshan Road, Xuhui District, Shanghai, 200030 China.

Phone: 86-21-64070434; E-mail: drwanglh0420@163.com.

† These authors contributed equally to this work.

(2)

blot

Color 55

43 34

A205 8

Hel a

MCF-7 THP1 marker

PD-L1≈ 40kd

merge A

A205 8

Hel

a MCF-7 THP1 marker

PD-L1≈ 40kd

43 34 26

GAPDH≈

36kd

A205 8

Hel a

MCF-7 THP1 marker

A205 8

Hel a

MCF-7 THP1 marker

blot

Color merge

(3)

B

blot

Color merge

marker B16F1 0

H22 Lewis U14

55 43 34

PD-L1≈ 40kd

marker

B16F1 0

H22 Lewis U14

PD-L1≈ 40kd

43 34 26

GAPDH≈

36kd blot

Color merge

marker B16F1 0

H22 Lewis U14

marker B16F1

0 H22 Lewis U14

(4)

Figure S1. The uncropped blot for the cropped band of western blot in figure 2.

A. The uncropped blot for figure 2B.

B. The uncropped blot for figure 2D.

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