Nostoc sp. PCC 7120 (J01540)
Halorhodospira halophilaDSM 244T(EF199945) Ectothiorhodospira marismortuiDSM 4180T(EF199942)
Ectothiorhodospira shaposhnikoviiDSM 243T(AY450587) Ectothiorhodospira haloalkaliphilaBN 9903T(EF199941) Ectothiorhodospira variabilis WN22T(EU503123)
Ectothiorhodospira mobilis DSM 237T(EF199939)
Ectothiorhodospira vacuolataDSM 2111T(EF199940) Allochromatium vinosum ATCC 17899TrbcA(M26396) Ectothiorhodosinus mongolicusM9T(EF199947)
Halorhodospira halochlorisDSM 1059T(EF199944) Halorhodospira abdelmalekii DSM 2110T(EF199943) Allochromatium vinosumATCC 17899TrbcL(D90204) Thiorhodospira sibiricaA12T(EF199948) 0.05
100
93 92
92
79
79
100
Supplementary Fig. S1. Phylogenetic position of strain WN22T among members of the Ectothiorhodospiraceaebased on conceptual translations of cbbL. The tree was generated by the maximum-likelihood method. Bar, 5 amino acid substitutions per 100 positions. Numbers indicate percentages of bootstrap sampling, derived from 1000 replications. Unrooted phylogenetic trees were constructed using algorithms implemented in the TREECONWsoftware package (http://bioc- www.uia.ac.be/u/yvdp/treeconw.html)
Gorlenko, V. M., Bryantseva, I. A., Rabold, S., Tourova, T. P., Rubtsova, D., Smirnova, E., Thiel, V. & Imhoff, J. F. (2009).Ectothiorhodospira variabilissp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes. Int J Syst Evol Microbiol 59, 658–664.
Nostocsp. PCC 7120 (AF012326)
Halorhodospira halophilaDSM 244T(EF199951)
Marichromatium purpuratum 95Car100a (AF059648) Thiorhodospira sibiricaA12T(EF202525)
Ectothiorhodospira vacuolataDSM 2111T(EF199955) Ectothiorhodospira mobilisDSM 237T(EF199954)
Ectothiorhodospira shaposhnikoviiDSM 243T(EF199953)
Ectothiorhodospira haloalkaliphilaBN 9903T(EF199956) Ectothiorhodospira variabilis WN22T(EU503124)
Halorhodospira abdelmalekii DSM 2110T(EF199952) Halorhodospira halochloris DSM 1059T(EF199950) 0.05
100
94 71
92 90
100
78
Supplementary Fig. S2. Phylogenetic position of strain WN22T among members of the Ectothiorhodospiraceae based on deduced amino acid sequences of nifH genes. The tree was constructed by the neighbour-joining algorithm with Nostocsp. PCC 7120 as the outgroup. Bar, 5 amino acid substitutions per 100 positions. Numbers indicate percentages of bootstrap sampling, derived from 1000 replications.
Gorlenko, V. M., Bryantseva, I. A., Rabold, S., Tourova, T. P., Rubtsova, D., Smirnova, E., Thiel, V. & Imhoff, J. F. (2009).Ectothiorhodospira variabilissp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes. Int J Syst Evol Microbiol 59, 658–664.