Dataset 2.4 The whole sequences of the five candidate genes in Part 2.
The start codon and stop codon are highlighted.
GT12, Tcas-al P450
>Tribolium castaneum GT12, Tcas-alkene-less P450 (Tcas-al P450), mRNA
ACGCGGGGACACTTCGAATTAGAACTCTAATAATGTATTTCGTAACTCCCGGTCTCATTTTTACGGTAATAGCACTTTGGTTGTTGTGGT CGTTCTTGTGGAAAAATTTTTTCGCTACGAAGATCAAAGGACCATGGGCTTTTCCGCTCATCGGGAGCGCCTACGTCCTGTTTGGCGGAA CACACAATATTTTGGAAAATACGGTCCGAGTGGTCAGCAAGTACCGGCCGTTGTGTAAACTGTGGTTGGGCAACAAGTTGACGATTTTCC CCTCGAACCCTGACGACATCCAGATGATCCTCAATAGGTCTTTGGAGAAGGAGGAGGGTTATAGGTACATCAAGACTGTTTTTGGACACG GCCTGTTCACTAGTCCCGTAAGCGAGTGGAAGGGGCATCGGAAAGTCATCGTTTCGACCCTCAACCAGCAAGTTTTGAACAGTTTTATGG ACACGTACAATATTTACACGCGGGAATTGGTCGAGAAACTCAAAATCGACACCAAGGACGGGCAGCATATCGACATGTTCCCCATTATGA CCCAGTGCACCTTGGATATCATCTGCAGCTCCATCATGGGGACGCAGATGAATGCCATGAAGGAGGAAAGTCAGTTTATTATGTGGATGA CAAGAGTGGCCGAGCTTGCAATCATTCGCATGTTCAACATCTACTTGTGGCCAGAACTCTTCTGGAAACTCAGCCCAATCTCGAAAGAGT CGCACGAGCTTGACCAAAAAATCTACAACTATGTCAAAACAGTGATACACAATAAGAGGCACAAGGGGTTGAAGTCGCACACTGGCAAGA AGATTTTCTTGGATTATTTGGTCGAATTGACTGACCGGGAGGGCCGATGGACTGACGAAGAGTTGACCAATGAAACCAGGAGTGTGATTA TGGCAGGAAGTGATGCCACAGCTTTGACCCTTTCCTATTGTCTTGTAATGCTGGCGATGTTTCAAGACATACAAGACAAGGTGTACGAGG AATTGTGTTCGGTTTTCGGTGACAGTGACCGGTTCGTGACCGTCGACGATTTGCCCCATTTGCAGTACTTGGATCGCTTTATTAAAGAGA CTTTGAGAGTTTTCCCAGTCACCTCAATGATCGGTCGCGAATTGACCACAGATATGACCATAGATGGGCATTTTATACCAAAAGGTACTT CAATTGGATTCCCCATTTTGTATATTCACAGGAACCCTGAATATTACCCCGACCCGCTCAAATTCGACCCGGATCGTTTCCTGCCGGAAG AAGTTGCCAAAAGACACCCTTGTACTTTTATTCCGTTTAGTTTTGGGCCGAGGAATTGCATTGGTTATAGGTACGCGATGATGACCATGA AAGTGATTCTTGCGACTCTGCTTAGGAGTTTCAAAATGGTGCACACCCCGTATAAAGAAATTTCAGAATTGAAAATTAAGTTTGATATTG CCACCAAAGTCGACGAAGGATATCCGGTGCGAATGGAGCTTCGCAAAAATGTGGCAGCTGGCACTAAATAGTTGTGTAGAGTTGGATTTT GATCAGAAATAAACGATTTCGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA
PS: the first ATG is the start codon of Tcas-al P450 variant A (Tcas-al P450A), and the second one Tcas-al P450 variant B (Tcas-al P450B).
GT64, TC015382
>Tribolium castaneum GT64, TC015382, mRNA
TAGTAACAGTGAAAAATGTCTAGCGAGCTAGCGCCCCTTAACATCCGACCAGTTTGGTTAAAAATCGTATATTTTGCGTACCTACATTAC GGTACCATTTTGGGTACTTACTACTTGTTAACAACGGCCCAATGGAAAACAATTCTATGGACCTATTTATTACTCCTTTCGGCCACTCAT GGTATTGCAGTGGGTGCCCATAGATTATGGGCCCATAGGGCTTACAAAGCCAAACTACCATTGCGCCTCCTTCTGGCCTTTGACCAGACT TTAACATTCCAGAAAGATATATATGATTGGGTTCGGGACCATCGAATCCATCACAAGTACTCTGACACAGAGTACGACCCCCATAACGCA ACCAGAGGGTTCTTTTACAGCCACATTGGATGGCTGATGATTAAAAAATCGGATAAAGTTATCGCAAAAGGGAAAGAACTAGACTTGAGT GATTTGGAGCAAGACCCAGTAGTATGGTACCAAAGAAAGTACTACTGGTATATGGCACCGTTTCTCGCCTTTATTTTCCCAGCTATGGTA CCATGGTACTTTTGGTCGGAACAGTTCAAAGTCTCATGGTACCTTTGCAGTATTTTTCGCTTATGTGTTACTCTACATGGTACTTGTTTG GTAAACAGTGCGGCCCATATTTGGGGCTCCAAACCATACGATAAAAATATTAAACCAGTGGAAACGTCGTGGGTGGCTCATATTAGTCAT GGTGAGGGTTGGCATAACTACCATCATGTCTTCCCATGGGATTATAAAGCTGCAGAATTGGGGTCATATTATGGCAATTGGAATACGGCC TTTATTGATTTTATGGCCAAAATTGGGTGGGCCTATGACCTTAAAAGCGCCCCATTGGACATGGTCAAAAAAAGGGGAGAGAGGACGGGC GATGGCACCAAATTGTGGGGCTGGGGGGATGAGGATATGGATAAGGAAGTTGTGTGTAAGGCGAAAAATGAATAAAGTTTTTATTTCTAA AAAAAAAAAAAAAAAAAAAAAAA
PS: 5’ end needs to be confirmed.
GT78, TC015349
>Tribolium castaneum GT78, TC015349, mRNA
TAGTCGAAACAAAAAATGGAACGTGAAATCGCGTGGAAGAAAGTCTTTTTCTTTGTGTATTTACATTTGGGGGCTTTGTACGGCTTGTAC CTCATCCTGACTGAAGCTTCGTGGTTGACCTGCTTTTGGTTCTATTTCCTGGTACTAATCTCGACGCAAGGTACCGGAGCAGGTGTCCAT CGCTTATGGGCACATAGGGCCTACAAGGCCACGGTACCACTTCGACTCTTACTAACATTTTACCAAACTTTAACCTTCCAAAAAGATATT TACGATTGGGTGCGCGACCATCGAGTCCATCACAAGTACTCTGACACGGAACCCGACCCACATAATGCCTCCAATGGGTTCTTTTACAGT CACATGGGTTGGTTAATGTTGAAAAAGACCCAATGTACCATCGATAAGGGCAAAAAATTGGACTTGAGTGACTTGGAAGCCGACCCAATC GTCATGTTTCAGAGAAAGTACTATTGGTACTTGGCACCGGTTATAGCCTTGGGAATGCCGGCCTTTGTACCGTGGTACTTTTGGGGTGAG AGATTTGTAGTTTCATGGTACGTCTGTAGCATGTTTAGGTACTGTCTAACCCTTCATGGTACCTGTCTGGTAAATAGCGCCGCTCATATT TACGGAAATCGCCCATATGACAAGAACATTTTACCAACGCAAAATTTGTTGGTCTCGTACATTACGAACGGAGAAGGCTTCCATAATTAC CACCATGCCTTCCCATGGGACTACAAAGCGGCCGAATTGGGGTCATATTATGGCAATTGGAGTACGGCATTTATTGATTTTATGGCCAGA ATTGGATGGGCATATGACCTTAAAAGTGTCCCATTGGCAATGGTCGAAAAGAGGATTAAAAGGACAGGTGATGGTACCCATAATGTGTGG GGATGGGGAGACAAGGATTTGCACCCAGATGACGCCAAAATGGCCGAAATTACACACACACGAGCTTCACAAACAGGATAATTG
PS: Both 5’ and 3’ ends need to be confirmed.
GT79, TC006231
>Tribolium castaneum GT79, TC006231, mRNA
CACAAAATGCCTCCACGATCCGAAGTGATACCCGTGTCCACTCTCAAAATCAAACCGCCTCCAAACCGGAGTCCGGCCCAAAACGGCGCTCTATGG CTCAAAGACAAGGAAGTGACCGATGGGGCTGAAGGCCTTTGGCGCATTCACGACGACCTATACGACTTCAGTGACTTCATCCGGAGCCACCCAGGC GGCTCCGAATGGCTCGAACTGACCAAAGGCACTGATATTACGGAAGCCTACGAAGTCCACCACTTGACCACAGCGCCCGAGCAAATTTTAAAGAAA TATCTAGTCAGAAAAGCCAAAACGAAGCGAAATTCGCCGTTTACGTTCAAAGATGATGGGTTTTATCGGACATTGAAACGCGAAGTTGCCTCAATT GTTAAAACTTTGCCCAAACAACCGATCAATAATTCGGCCACTTTTACCGATTTTCTGCTCGCTGGGACGTTTATGTTTGCGGTTTTGGCCAACACT TACTGGAATTATTACTTGGCGATTGTTGCCGGGGTTTTTCTCGGATGCCTGGTTATTGCAGCACATAATTATTTCCACAAGAAAGATAATTTTAGA ATGCTCTATTTCAACCTTTCTTTAATGCAAACAAGAGAGTGGCGAATCAGTCACGTGCTCAGCCACCACCTCCACACCAACACCATCGACGACATG GAGATCACCATGAACGAACCCCTCCTCCCATTCCTCCCCCATGAAAAGCCACCCATAATCAAATATGGCTACTACCTCTTCTTCCCCATTTACTGG CTCATAGCTTGGCACGCGAATTTCCTCAAAAGGGTCTATTACATCGCCAAGGGTGACACCCATTTAATCCTATGGTACGACTTCGTACCATACACC CTCCCACTTGCCATGTACCTAATCAGCGGCCAATCAATTTTTGCCACGTTATGGATGTGGACAGTCATTGTGGCAATTGGCAGTTTCCACTTCTCG GCAGTGGGTCTCAATGCCGCCCATCACCACCCAGACATCTTCCACGACGGTGATGCCCCACGGTCAGACACCGACTACGACTGGGGCTTGGGCCAA CTGGACGCTATCATGGATCGGAATGAAATCACAGGATCCCATTTTCTGGTCCTTACGAACTTTGGGGACCATGCCTTACACCACATGTTCCCAACT CTGGACCATGGCACTTTGGAGCTTTTGTACCCAACTTTCAAGAAAGTTTGCCAGGACTTTAACGTTGATCTGAGGATGACCTCGCAGTTGGAGTTG ATCAAAGGGTCTTTCATGCAAGTTATGAGGACCGAGCCCAATCCAAACCCGCCAGATTTGCTGAAGAAATATTAGCTGGTTATGCAACCAGCAGTT AACCGCCGCATTGTTGGTTGCCTAACTCGTCTATAAAAAATTTGAATGTAATTTCAAAATTAATTATTTATACGAAGTCTGGGCACGAGAATTGTT AATTGATGCTCATTGTAGGTAAATTGTGATACCGCCGTATTGTTAACCCGGCTTGTACTTTATTATGAATTAAAAGTTCATATCATATAAAAAAAA AAAAAAAAAAAAAAAAAAAAA
PS: 5’end needs to be confirmed.
GT80, TC006232
>Tribolium castaneum GT80, TC006232, mRNA
GATTGTTATCTTGTTACAAGTGACGAACGAACTTGTAGTGATTTTGGATTTTTTTGTGATTAATTAGCCACTAAGAGATG ACGGTGACAATCTCCAGATTTAGCTCAAGTCTTGGGATCAAATATCCCACTCTGAGGGACGACCCCTTGAAAAATGGGCTGCAGTGGCTGAAGGGT
AAGAGGGCCGATGACGGGGCCGAGGGCTTGTGGCGCGTTCACGATAATCTGTACGATTTGAGCGATTTTGTAAACCTACATCCAGGAGGAGCAGAG TGGCTACAGCTGACTAAAGGGACTGACATAACTGAAGCGTTCGAAACGCATCACTTGACCACAACAGCTGAAAAACTCGTCGAGAAATATTTTATT CGTAAAGCTAAGACTCCGCGAAATTCGCCGTTTACGTTCAAAGAGGGCGATTTTTACAAGACACTGAAGAGAAATATCGTTGAGAAATTAAAATAC GTGCCAAATGAACCGGCGCAAAGATCGAAGAGAATAACAGACTTTTTACTTTTTGCCTATGTTAGTTTGGCCCTTTTGGCCGCTGTTTTCCGAAGC TTTACGTTCGGAATGTTTTCGGGGATTTTTCTGAGTCTGACTGCTATCGCAGCTCATAATTTTTTCCATCAGAGGGACAATTTCAGGATGTATTAT TTTAACTTCACCTTGATGGAATACTCAGAGTGGCGTATAAGTCATGCGTTAAGTCATCGCTTGCATACCAACACCATCAATGATCTAGAAATATCA TCCCTGGAACCGTTCCTGGAATATCTTCCGGGAAAGAAGCACATCCTGGTCCAGCTCGCGCAAATTTTCATATCGCCAATTGTTTGGTGCTTTATA TTTTTGGGCTCTTTCATTCGAAGTTTGATTTCAACAATCCTCGGGGAAAAACCTTTCGAAATGGTCATTTTCCTCCCATTTAGCGTTCTAGCGGCC ATGGTGGTTCTCTCAGGCGAGATAATTTTCCCGCTTGTGATGTTTTCCTGTATTCAGCTGACTGGGAGTCTACATTTTGGGGCCGTGGGCCTGAAT GCGGCCCATCACCACCCGGAAATTTTCCACGACGGGGACACCCCAAGAAAAGAAAAAGATTGGGGGATTTACCAAATGGATGCGGTCATGGACCGT AAGGACATAACAGGGTCCCATTTTTTGGTACTGACTAATTTCGGTGACCATGCTTTACACCACTTATTTCCAACGATTGACCACGGTCTGTTGGAT TACTTGTATCCGACTTTTCTGAAAACTTGTGCCGACTTTGGCATTGAGTGGAGGCTTTCATCTCAGATTGAGTTGGTCAAAGGCCAGATTCGACAG CTGGTGAAGATAAAGCCGAACGAGGAGCCGCCAAGGGCACTCAAGCCGATCAAGTTTTTGTAATTATTGTTATTTAATAAAGTTGGGTTT ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
