Primer sequence 5׳3 ׳ Core Fragment
GgSqef1 GgSqer1
GATTGTGTGGAKGAAATYGATGC
׳ CGCATTTCHWKCCTWGCYKGATC RACE
RFISqepo RFOSqepo RRISqepo RROSqepo
GCATCAATTTCATCCACACAATC
׳ TGAAGCCTTTTCTCGCATTTTCTG׳
CATTAGCTGGTGCATTGTACAAAGT GAATCATTCTACACCCTACGCAAG Full length Gene
F2FLSQEPO R2FLSQEPO
ATGGATTATCAGTATATTCTGGGAGCGATT TCAATGGACAGGAGGTGCTCTGTAGTATGC Promoter
GWOSQEPO GWISQEPO
TCTGATGTCTTTGCACACCCATTACTTT AGGCCAGAATCGCTCCCAGAATATAC pEAQ-HT Transient Expression
Esqep Age1 EsqepSmaI
TTACCGGTATGGATTATCAGTATATTCTGGGAGC TCCCGGGATGGACAGGAGGTGCTCTGTAGTATG pBI121 Stable Expression
Esqepfp Esqeprp
TTTTCTAGAATGGATTATGAGTATATTGTCGGAGC TTTGAGCTCCTAATGGACAGGAGGTGCTCTGTAGTATG Real-Time expression
RTFActin RTRActin RTRSQE1 RTFSQE1 RTFSQE2 RTRSQE2 RTFSQE3 RTRSQE3 RTFSQE4 RTRSQE4 RTFSQE5 RTRSQE5
AGCCTGGATGGCAACATACATAGC ATGACATGGAGAAGATCTGGCATCA GGATTGTGTGGATGAAATT
GATGTGACAGTACCTTGTTCTA
ATGATGGACCCCTACGCGCTTGGATGCA CTATGCAGCCGGGGGAGTTCTGTAATAG TTGCTTCGATTATCTGAGTCTTGGAG ACAATGAAGGCAAAAACATTCCAACT AGTGTTGCGAAATCTTCTCAGGCCTTTG TTAAGCTTTAGGAGTGTTGAGTGAAGTC TGGTTCTCCATTTCTTTGCTGTTGCAGT TTATGAAGCCGGGGGGGCTCTGTAATAA
Supplementary Table 1 Primer sequences used in cloning and characterization of Squalene epoxidase gene (GgSQE) and promotor from Glycyrrhiza glabra. Real-time expression primers for all the five identified GgSQEs are also given along with Actin.
