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Contribution of Mevalonate and Methylerythritol Phosphate Pathways to Polyisoprenoid Biosynthesis in the Rubber-Producing Plant Eucommia ulmoides Oliver

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Contribution of Mevalonate and Methylerythritol Phosphate Pathways to Polyisoprenoid Biosynthesis in the Rubber-Producing Plant Eucommia ulmoides Oliver

Takeshi Bambaa,c,*, Michiko Murayoshib,c, Koichirou Gyoksenb,

Yoshihisa Nakazawac, Hiroshi Okumotod, Hiroko Kattod, Eiichiro Fukusakia, and Akio Kobayashia

a Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 - 1 Yamadaoka, Suita, Osaka 565-0871, Japan. Fax: +81-6-67 89-74 18.

E-mail: bamba@bio.eng.osaka-u.ac.jp

b Department of Forest and Forest Products Sciences, Faculty of Agriculture, Graduate Schools of Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan

c Hitachi Zosen Corporation, 2-2-11 Funamachi, Taisyou-ku, Osaka 551-0022, Japan

d Kurashiki University of Science and The Arts, Department of Chemistry and Bioscience, 2640 Nishinoura Tsurajima, Kurashiki, Okayama 712-8505, Japan

* Author for correspondence and reprint requests

Z. Naturforsch. 65 c, 363 – 372 (2010); received January 3/February 18, 2010

The biosynthetic origin of isopentenyl diphosphate in the polyisoprenoid biosynthesis of the rubber-producing plant Eucommia ulmoides Oliver was elucidated for the fi rst time by feeding experiments using 13C-labeled isotopomers of (RS)-mevalonate, 1-deoxy-D-xylulose- 3,4,5-triacetate, 2C-methyl-D-erythritol-1,2,3,4-tetraacetate, and pyruvate. After 13C-labeled isotopomers were fed to the young seedlings, the polyisoprenoid fractions were prepared and analyzed by 13C NMR. The NMR data showed that the isoprene units of polyisoprenoid derived from isopentenyl diphosphate, which was biosynthesized using both mevalonate and 1-deoxy-D-xylulose-5-phosphate in E. ulmoides. It is assumed that the cross-talk of isopente- nyl diphosphate, derived from both pathways, occurs during the biosynthesis of polyisopre- noid; therefore, it was observed in the formation of low-molecular weight isoprenoids.

Key words: Polyisoprenoid, Isopentenyl Diphosphate, Eucommia ulmoides

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