Analysis of Interactions between Carbohydrates and Proteins Using Fluorescent Labeling and SDS-PAGE Gang L. Huang* and Kongliang Wong

This thesis covers several aspects of the computational analysis of promoters and DNA- protein interactions: analysis of transcription factor binding sites (investigating position

At room temperature number of hydrogen bonded chromophores is much less than hydrogen nonbonded, therefore a small change of dissociation energy 3,2 kJ/mol cannot lead to a

The aims of the study were to determinate expression and function of two members of insulin – like family, namely Insl5 and Insl6 and to verify the interactions between PELO and its

a) Overexpress a fluorophore-tagged version of Syb2, like SpH, which selectively switches on upon fusion thereby giving a specific read-out for exocytosis. b) Optically resolve

The SILAC experiments performed in this study were aimed towards the quantitative identification of specific interaction partners of histones captured by in vivo crosslinking

This is due to the relatively high disorder of protein structures and the number of amino acids having different structural properties, which results in many degrees of freedom

Differential gel electrophoresis (DIGE) stains, immuno-stains and in-gel activity stains of protein complexes separated by BN- PAGE. A) DIGE staining of mitochondrial protein

Iron from pyoverdine was not incorporated through the major iron transporter IRT1 as indicated by the similar iron content of the wild-type plant and IRT1 mutant