Pancreatic Phospholipase A
2Ð Mediated Enhancement of the Respiratory Burst Response of Human Neutrophils
Julia Müller
#, Marijana Petkovic´
#,*, Jürgen Schiller and Jürgen Arnhold
Institute of Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Liebigstraße 27, D-04103 Leipzig, Germany.Fax: (+49) 341 97 15 709. E-mail: petm@medizin.uni-leipzig.de
* Author for correspondence and reprint requests
Z. Naturforsch.56c,1150Ð1156 (2001); received August 14/August 31, 2001
Human Neutrophils, Luminol-Amplified Chemiluminescence, Pancreatic Phospholipase A2 The aim of this study was to investigate the effects of exogenously added pancreatic phos- pholipase A2(pPLA2) on the production of reactive oxygen species by human polymorpho- nuclear leukocytes (PMNs). Pancreatic PLA2was used because PMNs do not possess a re- ceptor for that enzyme and, therefore, the receptor-mediated effects could be excluded.
Respiratory burst activity of PMNs was monitored by luminol-amplified chemiluminescence and the lipid composition of neutrophils after treatment with pPLA2 was determined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Our results show that the products of the pPLA2digestion of the PMN membraneÐlysophospholipids and the corresponding free fatty acidsÐsignificantly enhanced the respiratory burst response of human neutrophils.