Etablierung und Anwendung einer durchflusszytometrischen Methode für die Vitalitätsbestimmung von P. aeruginosa, B. cepacia und S. aureus in Mischkulturen

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VII

4.1.3.1.Optimierung der Vitalitätsfärbung für einzelne Spezies ... 86

4.1.3.2.Etablierung einer einheitlichen Färbemethode für die Vitalitätsbestimmung in Mischkulturen ... 94

4.1.4. Sind Bakterien mit intakter Membran vermehrungsfähig? ... 100

4.1.5. Spezies-spezifische Vitalitätsbestimmung in Mischkulturen ... 101

4.2. Vitalität und Wachstum in Rein- und Mischkulturen ... 104

4.2.1. Drei-Spezies-Mischkultur ... 104

4.2.2. Zwei-Spezies-Mischkulturen ... 109

4.2.3. Wachstumsanalyse mittels quantitativer DZM ... 118

5. Diskussion ... 122

5.1. Methode zur Vitalitätsbestimmung in Mischkulturen ... 122

5.1.1. Spezies-spezifische Detektion für die Mischkulturanalyse ... 123

5.1.2. Anpassung der Vitalitätsfärbebedingungen an die Spezies ... 125

5.1.3. Vierfarbenprotokoll für die Spezies-spezifische Bestimmung ... 129

5.2. Effekte der Mischkultivierung auf die Vitalität und das Wachstum von P. aeruginosa, B. cepacia und S. aureus ... 132

6. Zusammenfassung ... 139 7. Ausblick ... 141 Literaturverzeichnis ... 143 Abbildungsverzeichnis ... 158 Tabellenverzeichnis ... 160 Anhang ... i

A1 Eigene Publikationen und sonstige Beiträge ... ii

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