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Table S1. Primers used to amplify mitochondrial and nuclear markers. Most individuals (~ 80%) amplified COII successfully with primer combination Ringo/B–tLYS. The remaining ones required other combinations of forward (F) and reverse (R) primers. (

1

) Refers to the position of the primer’s 3’ base on the Drosophila yakuba GenBank X03240 (COI and COII) mtDNA genome. (

2

) The original sequence of primer Pat (S

IMON

et al. 1994) is 5’TCCAATGCACTAATCTGCCATATTA3’, which is different from primer Pat* used in this study. There are therefore at least two different primers with the same name and both appear to be popular. We have been unable to find the original reference for primer Pat*.

Gene Primer 3’ Position1 Sequence (5’ – 3’) Reference

COI LCO 1514 GGTCAACAAATCATAAAGATATTGG FOLMER et al. 1994

HCO 2173 TAAACTTCAGGGTGACCAAAAAATCA FOLMER et al. 1994

COI Jerry 2183 CAACAYTTATTTTGATTTTTTGG SIMON et al. 1994

Pat* 2 3014 ATCCATTACATATAATCTGCCATA ? 2

COII

F: Ringo 3039 AATATGGCAGATTATATGTAATGGAT This study

F: Lennon 3091 TTAGAAATGGCAACATGATC This study

R: McCartney 3768 CTTGCTTTCAGTCATCTAATG This study

R: Eva 3772 GAGACCATTACTTGCTTTCAGTCATCT BOGDANOWICZ et al. 1993 R: B–tLYS 3784 GGTTTAAGAGACCAGTACTTGC LIU & BECKENBACH 1992

GAPDH

HybFrigga – taatacgactcactatagggAARGCTGGRGCTGAATATG http://nymphalidae.utu.fi/Nymphalidae/Molecular.htm HybBurre – attaaccctcactaaagGWTTGAATGTACTTGATRAGRTC http://nymphalidae.utu.fi/Nymphalidae/Molecular.htm Sequencing primer F – TAATACGACTCACTATAGGG http://nymphalidae.utu.fi/Nymphalidae/Molecular.htm Sequencing primer R – ATTAACCCTCACTAAAG http://nymphalidae.utu.fi/Nymphalidae/Molecular.htm

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