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Inclusion criteria：

（1）breast cancer patients planning to undergo surgery

（2）women aged 18-65（including 18 and 65 years old）

（3）informed consent and voluntary participation in this clinical study

Exclusion criteria：

（1）use of antibiotics in the last one month

（2）history of mental or psychological problems, or are taking psychiatric medication or receiving professional psychotherapy

（3）history of cancer

（4）intestinal dysfunction (such as irritable Bowel Syndrome)

（5）metabolic diseases that affect the microbiota (e.g., thyroid dysfunction, diabetes, etc.)

（6）language communication difficulties

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