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(1)S4 Table Primer used for genotype Gene Forward Reverse Product size GGCTGCTCCCCTCCAACAT ATTGCGTTGCGCCATCTCAGTCAG Vgll4 flox TTCTTCTGGCCTCCATGGGCATTGT ATGTTATCAGCAGTCGGGCGGGAA WT:452bp

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Aktie "(1)S4 Table Primer used for genotype Gene Forward Reverse Product size GGCTGCTCCCCTCCAACAT ATTGCGTTGCGCCATCTCAGTCAG Vgll4 flox TTCTTCTGGCCTCCATGGGCATTGT ATGTTATCAGCAGTCGGGCGGGAA WT:452bp"

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S4 Table

Primer used for genotype

Gene Forward Reverse Product size

GGCTGCTCCCCTCCAACAT ATTGCGTTGCGCCATCTCAGTCAG

Vgll4 flox TTCTTCTGGCCTCCATGGGCATTGT ATGTTATCAGCAGTCGGGCGGGAA WT:452bp; Mut:616bp GACCACGTCTCCAAGATGAGCCGCA

GCATAATGGCCAGCAGAGGAGACTG GCCTCTGTCTTCCTGAAGTT TAACAGCGTGTCTCCTCCA

GCCTAACTCGGTGTCCATC TGTTCTGCTGGTAGTGGTC AAGGGAGCTGCAGTGGAGTA CCGAAAATCTGTGGGAAGTC GGCATTAAAGCAGCGTATCC CTGTTCCTGTACGGCATGG Primer used for quantitative RT-PCR

Gene Forward Reverse

Vgll4 ATGAACAACAATATCGGCGTTCT GGGCTCCATGCTGAATTTCC

Yap GCCATGCTTTCGCAACTGAA CAAAACGAGGGTCCAGCCTT Ctgf TCCGGACACCTAAAATCGCC TTCATGATCTCGCCATCGGG

Cyr61 AGAGGCTTCCTGTCTTTGGC CTCGTGTGGAGATGCCAGTT

Survivin AGAACAAAATTGCAAAGGAGACCA GGCATGTCACTCAGGTCCAA

Cyclin A2 GGCTGACACTCTTTCCG CTGGTAGCAAGAATTAGAGCAT

Cyclin B2 GAGAGTGAAGTCCTGGAA GTGCTGATCTTCAGGAGT

Cyclin D1 TGAGAACAAGCAGACCATCC TGAACTTCACATCTGTGGCA

Cdk4 GGGACATCAAGGTCACCCTA CGCTTAGAAACTGACGCATT Ki67 GCAGGTTAGCACTGTTATGAAAAC GGGCCTTGGCTGTTTTACATT

Gapdh GAAGGGCTCATGACCACAG GATGCAGGGATGATGTTCTG

WT:470bp; Mut:413bp

WT:350bp; Mut:470bp WT:405bp; Mut:749bp WT:297bp; Mut:196bp Vgll4 eGFP

Rosa RFP Vgll4 minus

Vgll4 LacZ TTCCCGCCCGACTGCTGATAACAT

TCCAGCTGAGCGCCGGTCGCTACCA

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