C A
RKOHCT 116
vector vector
circ-GALNT16
MigrationInvasionMigrationInvasion
Serpine1 vector Serpine1 100μm
sh-NC si-scramble
sh-circ-GALNT16 si-Serpine1 si-scramble si-Serpine1
DLD-1
0h
48h
LoVo
0h
48h
100μm
DLD-1Lovo
sh-NC si-scramble
sh-circ-GALNT16
MigrationInvasionMigrationInvasion
si-Serpine1 si-scramble si-Serpine1 100μm
D B
Migration Invasion 0
100 200 300 400
LoVo
Cellcounts
**
**
**
**
Migration Invasion 0
100 200 300
DLD-1
Cellcounts
** ** **
**
sh-NC + si-scramble sh-NC + si-Serpine1 sh-circ-GALNT16 + si-scramble sh-circ-GALNT16 + si-Serpine1
sh-NC + si-scramble sh-NC + si-Serpine1 sh-circ-GALNT16 + si-scramble sh-circ-GALNT16 + si-Serpine1
Migration Invasion 0
100 200 300 400
HCT 116
Cellcounts
**
**
** **
vector vector + Serpine1 circ-GALNT16 + vector circ-GALNT16 + Serpine1
**
Migration Invasion 0
100 200 300 400
RKO
Cellcounts
**
**
**
vector vector + Serpine1 circ-GALNT16 + vector circ-GALNT16 + Serpine1
DLD-1 LoVo
0 20 40 60 80
Migrationrate(%)
**
**
*
**
sh-NC + si-scramble sh-NC + si-Serpine1 sh-circ-GALNT16 + si-scramble sh-circ-GALNT16 + si-Serpine1
RKO HCT 116
0 20 40 60 80
Migrationrate(%) **
* *
* vector vector + Serpine1 circ-GALNT16 + vector circ-GALNT16 + Serpine1 vector
vector
circ-GALNT16
Serpine1 vector Serpine1
RKO
0h
48h
HCT 116
0h
48h
100μm
