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Note: Enzyme commission classification (Sinsabaugh et al., 2009; Cui et al., 2019a).

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Supporting Information

Table S1

Several extracellular enzymes representing soil C, N, P cycling are commonly used in ecoenzymatic stoichiometry models.

Ecoenzymes Abbreviation ECa Function

β-1, 4-Glucosidase BG 3.2.1.21 Cellulose degradation: hydrolyses glucose from cellobiose

β-D-Cellobiosidase CBH 3.2.1.91 Cellulose degradation: hydrolyses cellobiose dimers from non-reducing ends of cellulose molecules β-1, 4-N-Acetylglucosaminidase NAG 3.2.1.14 Chitin and peptidoglycan degradation: hydrolyses glucosamine from chitobiose

L-Leucine aminopeptidase LAP 3.4.11.1 Proteolysis: hydrolyses leucine and other hydrophobic amino acids from the N terminus of polypeptides

Acid or alkaline phosphatase AP 3.1.3.1 Hydrolyses phosphate from phosphosaccharides and phospholipids

Note: Enzyme commission classification (Sinsabaugh et al., 2009; Cui et al., 2019a).

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Fig. S1 Schematic example of the calculation of the RSSI-b score from areas under

enzyme activities curves over time. The lined area and the white-filled squares are

related to the contaminated disturbed (C

D

) soil, and the gray area and the black-filled

circles are the reference undisturbed soil (R

UD

). The ds acronym within the y-axis unit

refers to dry soil (Lessard et al., 2014).

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