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Fluorescence Resonance Energy Transfer (FRET)

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(1)

D * + A  D + A *

Fluorescence Resonance Energy Transfer (FRET)

(D = Donor, A = Akzeptor)

k

0

k k k

k k

k

k

FRET FRET ISC

IC F

FRET

FRET

FRET

= +

+ +

= + φ

0 0

,

k

k

F

F

=

φ

0

,

k k

k

FRET F FRET

F

= +

Mit und φ folgt:

0 , , 0

,

,

1

1

F FRET F

F FRET F

FRET

I

I

=

= φ

φ φ

(2)

D E A

λ

Fluorescence

D E A

λ

Fluorescence

FRET Efficiency, E:

6

0

1 1

E

R R

=  

+  

 

R = D-A distance R0 = Försterradius

(D-A distance where E = 50%)

0.5*R0

R0 (typical: 4-7 nm)

1.5*R0

Distance R

FRET

(E )

100%

50%

0%

Fluorescence Resonance Energy Transfer (FRET)

Messung von Abständen im Bereich von 2 – 10 nm

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