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M ETAGENOMIC  L IBRARIES   S UPPLEMENT ‐H:   R ESULTS FROM  C ONSTRUCTION AND SCREENING OF

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S UPPLEMENT ‐H:   R ESULTS FROM  C ONSTRUCTION AND SCREENING OF 

M ETAGENOMIC  L IBRARIES  

 

Construction of small insert libraries was successful. However, after the screening, only three  positive clones for protease activity was noticed. As for the esterase, no clone showed significant  activity. The positive clones for protease activity the phenotype was found to be unstable as after  picking the colony from the plate and streaking on a new plate no activity was observed.  

 

TABLE  43:  CHARACTERIZATION  OF  CONSTRUCTED  METAGENOMIC  LIBRARIES  (SMALL  INSERT  LIBRARY)  AND SCREENING FOR FUNCTIONAL GENES 

Library  Number of clones  (with insert)  

Average  insert size  (kb)  

Insert  frequency  (%)  

Estimated  library size (Mb) 

Screening  cover (Mb)  

Detected  positive  clone  Protease 

enrichment  

68220  3.03 98.42 206.7 212.09  3

Lipase  enrichment 

76680  1.83 97.65 140.3 146.37  0

 

On  the  other  hand,  construction  of  large  insert  library  from  cellulase  enrichment  was  unsuccessful.  

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