Y. Sequence of AJ300094
7. Zusammenfassung
Eine Kollektion letaler P-Insertionslinien, die bei der systematischen Suche zur Identifikation von essentiellen, X-chromosomal lokalisierten Genen isoliert worden sind, wurden mittels Klonierung genomischer DNA-Sequenzen um die Insertionsstelle des P-Elements kartiert. Es wurde die Lage der Insertionsstelle innerhalb der genomischen DNA bestimmt und das Expressionsmuster des Reportergen-Konstrukts charakterisiert. Die flankierenden “STSs” wurden der EMBL Nukleotid-Datenbank zu Verfügung gestellt und die in-situ-Daten zusammen mit den „enhancer trap“-Expressionsmustern über die FlyView-Datenbank zugänglich gemacht. Die so charakterisierten Linien sind für die wissenschaftliche Gemeinschaft im Internet abrufbar und ermöglichen eine detailierte Analyse der X-chromosomal-kodierten Genfunktionen.
Eines der Gene auf dem X-Chromosom ist das Drosophila Wnt5-Gen (DWnt5). Es wurden zwei unabhängige letale und drei homozygot lebensfähige Allele isoliert, welche die Identifikation von zwei unterschiedlichen Funktionen von DWnt5 in der Fliege erlaubten.
Beobachtungen des sich entwickelnden Nervensystems in mutanten Embryonen legen nahe, daß die DWnt5-Aktivität die Ausprägung des Axonmusters beeinflusst. Experimentell induzierte, erhöhte Aktivität von DWNT5 in den Zellen der ZNS-Mittellinienzellen stören die Bildung und Erhaltung der neuroektodermalen Zellinie. Die Analyse der Expression und des mutanten Phänotyps zeigen, daß DWnt5 benötigt wird, um ein intaktes Nervensystem zu bilden.
Eine zweite und unter allen bisher beschriebenen WNT-Molekülen neue Funktion ist die Kontrolle der Körpergröße durch Regulation der Zellzahl. Erhöhte Mengen von DWNT5 in einer eigentlich post-mitotischen Region der Augen-Imaginalscheibe initiieren eine oder mehrere zusätzlichen Mitosen. Dies führt zu überzähligen Ommatidien im adulten Auge.
Die erhöhte Zellzahl und der Effekt auf den Zellzyklus nach experimentell erhöhter WNT5-Aktivität ist das Resultat ausbleibender Herunteregulation von cyclinB. Dadurch kann eine neue Zellteilung erfolgen und der Übergang der betroffenen Zellen in eine G1-Ruhephase des Zellzyklus bleibt aus.
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Foteini Mourkioti
1979-1986: Primary School, Nea Manolada, Ilias, Greece Parents: Nikolaos Mourkiotis and Vassiliki Mourkioti
Nationality: Greek
1986-1989: 19th Gymnasium, Patras, Greece 1989-1993: 3rd General Lyceum, Patras, Greece 1994-1998: Bachelor in Biology (Note: very good)
Faculty of Biology,
University of Patras, Greece
1997-1998: Diploma thesis (Note: Excellent):
“Molecular analysis of the subclones of the neural specific gene chiP12-13”
Department of General Biology and Molecular Genetics, Faculty of Medicine, University of Patras, Greece
Supervisor: Prof. Dr. Aglaia Athanassiadou
1998-2002: Dissertation :
"Functional genomics of the Drosophila X-Chromosome and the role of DWnt5 during development".
Max-Planck Institute for Biophysical Chemistry, Department Molecular Developmental Biology,
Karl-Friedrich Bonhoeffer Institute, Goettingen, Germany
Supervisor: Prof. Dr. Herbert Jäckle
Peter A, Schottler P, Werner M, Beinert N, Dowe G, Burkert P, Mourkioti F, Dentzer L, He Y, Deak P, Benos PV, Gatt MK, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demaille J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Minana B, Kafatos FC, Bolshakov S, Siden-Kiamos I, Papagiannakis G, Spanos L, Louis C, Madueno E, de Pablos B, Modolell J, Bucheton A, Callister D, Campbell L, Henderson NS, McMillan PJ, Salles C, Tait E, Valenti P, Saunders RD, Billaud A, Pachter L, Klapper R, Janning W, Glover DM, Ashburner M, Bellen HJ, Jackle H, Schafer U.
Mapping and identification of essential gene functions on the X chromosome of Drosophila.
EMBO Rep. 2002 Jan; 3(1): 34-8.
Benos PV, Gatt MK, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demaille J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Minana B, Kafatos FC, Bolshakov S, Siden-Kiamos I, Papagiannakis G, Spanos L, Louis C, Madueno E, de Pablos B, Modolell J, Peter A, Schottler P, Werner M, Mourkioti F, Beinert N, Dowe G, Schafer U, Jackle H, Bucheton A, Callister D, Campbell L, Henderson NS, McMillan PJ, Salles C, Tait E, Valenti P, Saunders RD, Billaud A, Pachter L, Glover DM, Ashburner M.
From first base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies.
Genome Res. 2001 May; 11(5): 710-30.
Benos PV, Gatt MK, Ashburner M, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demailles J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Minana B, Kafatos FC, Louis C, Siden-Kiamos I, Bolshakov S, Papagiannakis G, Spanos L, Cox S, Madueno E, de Pablos B, Modolell J, Peter A, Schottler P, Werner M, Mourkioti F, Beinert N, Dowe G, Schafer U, Jackle H, Bucheton A, Callister DM, Campbell LA, Darlamitsou A, Henderson NS, McMillan PJ, Salles C, Tait EA, Valenti P, Saunder RD, Glover DM.
From sequence to chromosome: the tip of the X chromosome of D.
melanogaster.
Science. 2000 Mar 24; 287(5461): 2220-2 Announcments
Gronke, S., Mourkioti, F., Hader, T., Steuernagel, A., Eulenberg, K., Bronner, G., Jackle, H., Kuhnlein, R.
Regulation of energy homeostasis in Drosophila.
Annual Drosophila Research Conference, 2001, Washington D.C. , USA (Poster) Mourkioti, F., Peter, A., Schafer, U., Jackle, H.
DWnt-5 function in the developing nervous system.
Annual Drosophila Research Conference, 2001, Washington D.C. , USA (Poster) Mourkioti F., Peter A., and Jäckle H.
Analysis of DWnt-5 function during CNS development 3rd GfE, 2000, Günzburg, Ulm, Germany (Poster).