A Ampere
ACTB Beta-Actin
Ag Antigen
AK Antikörper
Ang II Angiotensin II
ANP Atriales natriuretisches Peptid
APS Ammoniumperoxidisulfat
AS Aortenstenose
AS-Gruppe Patienten mit aortaler Stenose
BCA-Kit Farbstofflösung Sigma
BNP Brain natriuretisches Peptid
BSA Bovines Serum Albumin
cDNA copy Desoxyribonukleinsäure
CnA Untereinheit-Calcineurin A
CnB Untereinheit-Calcineurin B
c-fos Proto-Onkogen
c-jun Proto-Onkogen
Cnr Calcineurin
Ct–Wert Wert des Treshold Cycle
DCM Dilatative Kardiomyopathie
DCM-Gruppe Patienten mit dilatativer Kadiomyopathie
DNA Desoxyribonukleinsäure
dNTP Dinukleotidtriposphat
DTT Dithiothreitol
EDVI/ESVI Enddiastolischer/endsystolischer
Volumenindex
EF% Ejektionsfraktion in Prozent
FG Feuchtgewicht
KHK-Gruppe Patienten mit Koronare Insuffizienz
Ko Kontrollen
MAPKs Mitogen activated protein kinase
Me Median
mRNA messenger-Ribonukleinsäure
MW Mittelwert
NFATc4 Nuclear factor of activated T cells
norm./cal normalisiert/kalibriert
n.s. nicht signifikant
NS Nerven-System
OD optische Dichte
PAGE Polyacrylamidgelelektrophorese
PCR Polymerase Kettenreaktion
PDH Pyruvat-Dehydrogenase
PIC Proteaseinhibitoren–Cocktail
PKC Proteinkinase C
PLC Phospholipase C
SD Standard-Abweichung
SDS Sodiumdodecylsulfat
SERKA Sarkoplasmatische
Ca++-Adenosintriphosphatase
SR Sarkoplasmatisches Retikulum
TBS-T Puffer TRIS buffered Saline-Tween Puffer
TEMED Tetramethylethylendiamin
TGF Transforming growth factor
TNFa Tumor Nekrose Faktor a
UV-Licht Ultraviolettes Licht
VW Vorderwand
V Volt
5.2 Materialien
(10x CYBR® Puffer, Amp-Erase (1U/µl), AmpliTaq Gold™ (5U/µl), dNTPMix (2,5mMdATP, 2,5mM dCTP, 2,5mM dGTP, 2,5mM dUTG und MgCl2 (25mM))
ABI
Comassieblue Farblösung Sigma
DEPC-Lösung0,1% autoklaviert selfmade ; Stammlsg. Sigma
DNAse Roche
Fwd/Rev. Primer CnB, BNP, ANP, GAPDH Invitek
Glycerol Roth
Glycin Roth
Isopropanol Roth
KCl Roth
LUCENTTM HRP-Substratlösung Chemikon
Methanol Roth
MgCl2 Roth
MnCl2 (8mM) Roth
Monoclonal anti-Calcineurin (ß-Subunit) SIGMA
Nonident p-4c Sigma
PIC (Leupeptin, Aprotinin, Pepstatin) Sigma
PMFS Sigma
Random-Hexamer-Primer, Stocklösung GIBCO BRL
Roti® -Load 1 Proteinauftragspuffer Roth
RNAsin Promega
SDS (10%) Roth
Superscript (200 U/l) GIBCO BRL
TEMED Roth
TRIS-Base Roth
Tris HCl Roth
Tween MERK
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