Fig. S1: mESCs undergo morphological changes during induced neuronal differentiation. wt mESC and monoclonal lines A6, B4 and C6 during early neuronal differentiation. Pictures taken with an optical reflected light microscope.
day 1 day 2 day 3
wtclone B4clone C6clone A6
undifferentiated
193 5.2 Supplementary table
Tab. S1: List of selected candidates for cap-independent translation initiation. Candidates have been identified by proteomic analysis in mESCs after 24 h of inhibition of cap-dependent translation by dominant negative 4E-BP1 overexpression. Fold change compared to mESCs.
A6 B4 C6 combined
24 h candidates
5'UTR length
splice sites
fold
change p-value fold
change p-value fold
change p-value fold
change p-value GO term function
Atp5e 109 2,6 0,384 2,6 0,386 2,9 0,340 2,7 0,080 ATP synthase hydrolase
Brwd3 219 2,0 0,250 2,0 0,255 1,8 0,276 1,9 0,043 chromatin modifying?
Ccdc47 213 1 3,3 0,000 3,1 0,000 1,1 0,316 2,5 0,011 osteoblast differentiation, ER overload response
Cdkn1c 208 1 2,2 0,153 1,7 0,032 3,1 0,005 2,3 0,011 cell cycle
Chek1 513 -0,1 0,856 1,2 0,351 2,5 0,024 1,2 0,223 protein phosphorylation
Dclk2 682 1,9 0,051 2,4 0,003 2,3 0,058 2,2 0,005 phosphorylation, intracellular signal transduction
Ero1lb 194 0,5 0,013 2,4 0,002 -0,2 0,631 0,9 0,250 oxidation-reduction process
Fam96b 275 1,9 0,147 1,9 0,149 2,2 0,112 2,0 0,006 chromosome segregation,
iron-sulfur cluster assembly
Gtf2a1 445 2,0 0,147 2,4 0,133 2,0 0,170 2,1 0,042 transcription
Hsbp1 38 2,6 0,001 1,1 0,220 2,4 0,005 2,0 0,007
cellular response to heat, muscle contraction, endodermal cell differentiation
Lama1 70 3,3 0,047 3,1 0,057 3,1 0,059 3,2 0,000 cell adhesion
194
Lsm6 203 1 1,5 0,385 1,7 0,343 1,7 0,343 1,6 0,072 mRNA splicing factor
Lsm7 23 2,1 0,230 3,4 0,073 1,6 0,381 2,4 0,128 mRNA splicing factor
Luzp1 397 3 2,0 0,127 1,7 0,175 2,2 0,106 2,0 0,006 ---
Palld 203 1 2,8 0,000 0,9 0,112 0,3 0,571 1,3 0,114 cell migration, actin cytoskeleton organization
Pcdh1 230 1 1,7 0,241 3,1 0,003 3,0 0,003 2,6 0,006 cell adhesion
Pex5 75 1 2,5 0,014 0,5 0,468 0,6 0,517 1,2 0,136 cell development
Pqbp1 582 1,7 0,328 1,6 0,354 1,9 0,294 1,7 0,056 transcription cofactor
Rad51 230 2,1 0,353 3,2 0,109 2,0 0,361 2,4 0,081 DNA strand-pairing hydrolase
Riok1 472 2,1 0,125 2,2 0,121 2,0 0,145 2,1 0,005
rRNA processing, phosphorylation
Sh3glb2 111 0,9 0,314 0,7 0,374 2,1 0,039 1,2 0,070 ---
Stmn2 252 2,9 0,352 2,0 0,521 3,1 0,333 2,7 0,112 tubulin binding
Tfe3 718 2 2,5 0,130 2,8 0,101 3,1 0,083 2,8 0,003
transcription, osteoclast
differentiation, humoral immune response
Zfp808 110 2,0 0,020 1,3 0,129 0,3 0,656 1,2 0,071 transcription
Znhit6 136 1,6 0,153 2,0 0,109 1,5 0,182 1,7 0,010 transcription factor binding
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