4 Climate change impairs nitrogen cycling in European beech forests
4.4 Material and Methods
4.4.15 Statistics
[APE]: Atom% excess of pool X
[ ] = - N atomic % excess immediately after 15N labelling [ ] - N atomic % excess six hours after labelling.
Plant uptake [mg N lysimeter-1] [ ]
([ ] [ ] )
Microbial inmobilization [mg N kg-1 sdw day-1] [ ] [ ]
([ ] [ ] )
Microbial immobilization [mg N kg-1 sdw day-1] [ ] [ ]
([ ] [ ] )
All N turnover rates were transferred to the unit mg N m-2 day-1, considering total dry soil contained in the beech-soil-mesocosm and the surface area. Plant uptake rates were transferred to the same unit by considering the surface area of the beech-soil-mesocosm.
4.4.15 Statistics
Experimental data is generally shown as mean values with standard errors (SE) of the mean, if not otherwise stated. Data were log-transformed if necessary to meet the requirement of normality and subsequently analyzed using two-way ANOVA with the factors exposure (NW versus SW exposure, i. e. control and climate change treatments), time and their interaction.
Depending on the parameters investigated, the factor time has different levels with independent observations, since samples were taken from different beech-seedling-soil mesocosms. There were two levels for gross rates of N turnover (June, September), three levels for N pools and microbial N cycle gene abundance (June, August, September) and five levels for isotopic composition of C and N pools (June_6 hours after labelling; June_48 hours after isotope labelling; August_6hours after isotope labelling; August_48 hours after isotope labelling and September_3months after isotope labelling). Differences between the levels of the time factor within each treatment level were further tested by applying the Tukey post-hoc test. For plant metabolites in fine roots, single comparisons between SW and NW were performed with non-parametric u-tests due to smaller number of replicates.
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