3. Results and Discussion
5.4. DNA polymerase activity studies
5.4.6. Recognition of remote mismatches
In order to probe effects of mismatched primer/template complexes located up to 5 bp proxyterminal to the 3´-primer terminus, as depicted in the appropriate figure in the results part were employed. Reaction setup was conducted as described in 5.5.3.
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Websites
S1 www.ncbi.nlm.nih.gov/sites/entrez S2 www.expasy.org/sprot/
S3 www.faculty.ucr.edu/~mmauro/codonusage/usage.htm
7. Appendix
dCTP 2´-deoxycytidine 5´-triphosphate dGTP 2’-desoxyguanosine-5'-triphosphateEDTA ethylendiaminetetraacetic acid Exo exonuclease activity
FPLC fast performance liquid chromatography
g gramme
G guaninosine
h hour; human
IPTG isopropyl β-D-1-thiogalactopyranoside
k rate constant
Kcat maximal catalytic speed
kDa kilodalton
KF exo- Klenow fragment of E. coli DNA-Polymerase I (3´-5´-exo-) kcat rate constant of the catalysed reaction
KM Michaelis-Menten-constant
l litre
LB Luria-Bertani
7. Appendix
NTP nucleotide-5’-triphosphate
O oxygen
PAGE polyacrylamide-gel electrophoresis PCR polymerase chain reaction
pM picomolar
pmol picomole
Pol DNA polymerase
polA* D. radiodurans DNA polymerase A truncated form
rel. relative
SSB (protein) single-strand binding (protein) ssDNA single-stranded DNA
t time
T thymidine
Taq Thermus aquaticus
Pfu Pyrococcus furiosus
TEMED N, N, N’, N’-tetramethylendiamine Tris tris-(hydroxymethyl)-aminomethane
TTP thymidine-5'-triphosphate
U units, uridine
UTP uridinetriphosphate
UV ultraviolet
V volt
Vmax maximal catalytic speed