Plasmid

Table 4 Plasmids used in this study

Plasmid Properties Source or references

pIND3 Insertion duplication mutagenesis vector for N. gonorrhoeae; Erm^R

[16]

pET20b(+) Expression vector, T7 promoter; Amp^R Merck Millipore pKS001 Plasmid, which is used as template to construct

different tsaP mutants by site directed mutagenesis

This study

pKS004 Plasmid to construct tsaPΔS -V245 deletion created by site-directed mutagenesis using primers 1007 and 1008

This study

pKS007 pSJ023- tsaP; Cm^R This study

pKS011 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-407) created with primers 1066 and 1067, cloned in KpnI and PstI site of pKT25; Kan^R

This study

pKS012 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa152-407) created with primers 1074 and 1067, cloned in KpnI and PstI site of pKT25; Kan^R

This study

pKS013 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-151) created with primers 1066 and 1069, cloned in KpnI and PstI site of pKT25; Kan^R

This study

pKS014 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-407) created with primers 1068 and 1067, cloned in KpnI and PstI site of pKNT25; Kan^R

This study

pKS015 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa152-407) created with primers 1065 and 1067, cloned in KpnI and PstI site of pKNT25; Kan^R

This study

pKS016 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-151) created with primers 1068 and 1069, cloned in KpnI and PstI site of pKNT25; Kan^R

This study

pKS017 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-407) created with primers 1068 and 1067, cloned in KpnI and PstI site of pUT18C; Amp^R

This study

pKS018 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa152-407) reated with primers 1065 and 1067, cloned in KpnI and PstI site of pUT18C; Amp^R

This study

pKS019 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-151) created with primers 1068 and 1069, cloned in KpnI and PstI site of pUT18C; Amp^R

This study

pKS020 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-407) created with primers 1068 and 1067, cloned in KpnI and PstI site of pUT18; Amp^R

This study

pKS021 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa152-407) created with primers 1065 and 1067, cloned in KpnI and PstI site of pUT18; Amp^R

This study

pKS022 Plasmid used for BACTH analysis. Contains PCR product encoding TsaP(aa23-151) created with primers 1068 and 1069, cloned in KpnI and PstI site of pUT18; Amp^R

This study

pKS023 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-715) created with primers 1070 and 1071, cloned in KpnI and BamHI site of pKT25;

Kan^R

This study

pKS024 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-300) created with primers 1070 and 1072, cloned in KpnI and BamHI site of pKT25;

Kan^R

This study

pKS025 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa301-715) created with primers 1073 and 1071, cloned in KpnI and BamHI site of pKT25;

Kan^R

This study

pKS026 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-715) created with primers 1070 and 1071, cloned in KpnI and BamHI site of pKNT25;

Kan^R

This study

pKS027 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-300) reated with primers 1070 and 1072, cloned in KpnI and BamHI site of pKNT25; Kan^R

This study

pKS028 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa301-715) reated with primers 1073 and 1071, cloned in KpnI and BamHI site of pKNT25;

Kan^R

This study

pKS029 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-715) created with primers 1070 and 1071, cloned in KpnI and BamHI site of pUT18C;

Amp^R

This study

pKS030 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-300) created with primers 1070 and 1072, cloned in KpnI and BamHI site of pUT18C;

Amp^R

This study

pKS031 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa301-715) created with primers 1073 and 1071, cloned in KpnI and BamHI site of pUT18C;

Amp^R

This study

pKS032 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-715) created with primers 1070 and 1071, cloned in KpnI and BamHI site of pUT18;

Amp^R

This study

pKS033 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa25-300) created with primers 1070 and 1072, cloned in KpnI and BamHI site of pUT18;

Amp^R

This study

pKS034 Plasmid used for BACTH analysis. Contains PCR product encoding PilQ(aa301-715) created with primers 1073 and 1071, cloned in KpnI and BamHI site of pUT18;

Amp^R

This study

pKS035 Vector for overexpression of 10x his-tagged TsaPΔA -R83; Amp^R

This study

pKS036 Plasmid to construct tsaPΔA -R83 deletion. Created This study

by site-directed mutagenesis on pKS007 using primers 1107 and 1108; Cm^R

pAW001 Vector for overexpression of 10x his-tagged TsaP;

Amp^R

Alexander Wagner pAW003 Vector for overexpression of HA-tagged PilQ(B1/B2);

Amp^R

Alexander Wagner

pSJ023 Vector for overexpression of OneSTrEP tagged SsbB (derived from pKH37 vector); Cm^R

Samta Jain pSJ082 Plasmid to construct tsaP deletion vis

insertion-duplication mutagenesis; Erm^R

Samta Jain

pSC108 Vector for overexpression of 6x his-tagged PilQmx aa20-656; Kan^R

Carmen Friedrich

pSC121 Vector for overexpression of MalE-tagged TsaPmx; Amp^R

Carmen Friedrich pKNT25 Bacterial Adenylate Cyclase Two-Hybrid System vector;

Kan^R

EUROMEDEX

pKT25 Bacterial Adenylate Cyclase Two-Hybrid System vector;

Kan^R

EUROMEDEX pUT18 Bacterial Adenylate Cyclase Two-Hybrid System vector;

Amp^R

EUROMEDEX

pUT18C Bacterial Adenylate Cyclase Two-Hybrid System vector;

Amp^R

EUROMEDEX

Im Dokument One ring to rule them all : Identification and characterization of the type IV pili secretin associated protein TsaP and analysis of the type IV secretion system of Neisseria gonorrhoeae (Seite 38-41)