Origin of the 67 EHV-1 abortion cases, sample character, processed sam-

10.2 Abortion cases

10.2.1 Origin of the 67 EHV-1 abortion cases, sample character, processed sam-

of the ORF 30 amplicons for sequencing

Designation Year Source Origin Sample Processed ORF 30 REASal I Sequencing character sample nested PCR

T952 1987 Neubauer single case virus isolate DNA positive positive yes

E510/89 1989 Neubauer single case virus isolate DNA positive negative no E525/89 1989 Neubauer single case virus isolate DNA positive positive yes S147/90 1990 Neubauer single case virus isolate DNA positive negative no F553/89 1990 Neubauer single case virus isolate DNA positive positive yes KermannNr.1 1991 Neubauer single case virus isolate DNA positive negative no KermannNr.2 1991 Neubauer single case virus isolate DNA positive negative no U169-71 2003 Neubauer single case virus isolate DNA positive negative no

260.3 2003 Neubauer single case virus isolate DNA positive negative no

Y30 2003 Neubauer single case virus isolate DNA positive negative no

T759/61 2003 Neubauer single case virus isolate DNA positive negative no (A)E271-3 2004 Neubauer single case virus isolate DNA positive positive yes S138/04 2004 Virology single case virus isolate DNA positive negative no

FU Berlin

S155/04 2004 Virology single case virus isolate DNA positive negative no FU Berlin

S415/04 2004 Virology single case virus isolate DNA positive positive yes FU Berlin

S587/04 2004 Virology single case virus isolate DNA positive negative yes FU Berlin

A258 2004 Neubauer single case virus isolate DNA positive positive yes

E854/6 2006 Neubauer single case virus isolate DNA positive negative no E216-18 2006 Neubauer single case virus isolate DNA positive negative no E573-5 2006 Neubauer single case virus isolate DNA positive negative no E113-115 2006 Neubauer single case virus isolate DNA positive negative yes

110113

Designation Year Source Origin Sample Processed ORF 30 REASal I Sequencing character sample nested PCR

FU Berlin

S173/06 2006 Virology single case virus isolate DNA positive negative yes FU Berlin

S208/06 2006 Virology single case virus isolate DNA positive negative no FU Berlin

S101/07 2007 Pathology single case virus isolate DNA positive negative no Dr. Haider

S144/07 2007 Pathology single case virus isolate DNA positive negative no FU Berlin

S152/07 2007 Pathology single case virus isolate DNA positive negative no Dr. Haider

S245/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

S260/07 2007 Pathology single case virus isolate DNA positive negative no FU Berlin

S275/07 2007 Pathology outbreak virus isolate DNA positive negative yes

FU Berlin stud farm 1

S276/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

S299/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

V07-5-61 2007 Pathology outbreak virus isolate DNA positive negative no FU Berlin stud farm 1

S309/07 2007 Pathology outbreak virus isolate DNA positive negative yes

FU Berlin stud farm 1

S319/07 2007 Pathology outbreak virus isolate DNA positive negative no

111114

Designation Year Source Origin Sample Processed ORF 30 REASal I Sequencing character sample nested PCR

FU Berlin stud farm 1

E1470/07 2007 Pathology outbreak virus isolate DNA positive negative no FU Berlin stud farm 1

E1471/07 2007 Pathology outbreak virus isolate DNA positive negative no FU Berlin stud farm 1

E1472/07 2007 Pathology outbreak virus isolate DNA positive negative no FU Berlin stud farm 1

S335/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

E1606/07 2007 Pathology outbreak virus isolate DNA positive negative no FU Berlin stud farm 1

S384/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

E1730/07 2007 Pathology outbreak virus isolate DNA positive negative no FU Berlin stud farm 1

S471/07 2007 Pathology outbreak virus isolate DNA positive negative yes

FU Berlin stud farm 1

S476/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

S477/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

S483/07 2007 Pathology outbreak virus isolate DNA positive negative no

FU Berlin stud farm 1

S38/08 2008 Pathology outbreak virus isolate DNA positive negative no

Dr. Haider stud farm 2

112115

Designation Year Source Origin Sample Processed ORF 30 REASal I Sequencing character sample nested PCR

Dr. Haider stud farm 2

S49/08 2008 Pathology outbreak virus isolate DNA positive negative no

Dr. Haider stud farm 2

S55/08 2008 Pathology outbreak virus isolate DNA positive negative no

Dr. Haider stud farm 2

S59/08 2008 Pathology outbreak virus isolate DNA positive negative no

Dr. Haider stud farm 2

S150/08 2008 Pathology outbreak virus isolate DNA positive negative yes

Dr. Haider stud farm 2

S261/08 2008 Pathology single case virus isolate DNA positive negative yes S72/09 2009 Pathology single case virus isolate DNA positive negative no

FU Berlin

V09-3-2/ 2009 stud outbreak nasal swab DNA positive positive yes

Mare I veterinarian stud farm 3

V09-3-3/ 2009 stud outbreak nasal swab DNA positive positive yes

Mare II veterinarian stud farm 3

S159/09 2009 Pathology single case virus isolate DNA positive negative no FU Berlin

S238/09 2009 Pathology single case virus isolate DNA positive negative no Dr. Haider

S604/09 2009 Pathology single case virus isolate DNA positive negative no FU Berlin

PA284 2009 Koblenz single case virus isolate DNA positive negative no

PA332 2009 Koblenz single case virus isolate DNA positive negative no

624 2009 Saxony-Anhalt single case virus isolate DNA positive negative no 647 2009 Saxony-Anhalt single case virus isolate DNA positive negative no

113116

Designation Year Source Origin Sample Processed ORF 30 REASal I Sequencing character sample nested PCR

681 2009 Saxony-Anhalt single case virus isolate DNA positive negative no

470/Mare C 2009 stud outbreak virus isolate DNA positive negative yes

veterinarian stud farm 4

541/Mare F 2009 stud outbreak virus isolate DNA positive negative yes

veterinarian stud farm 4

Mare A 2009 stud outbreak placenta DNA positive negative yes

veterinarian stud farm 4

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Stud farm 3: The samples listed below came from two in the table above already considered abortion cases (V09-3-2/MareI, V09-3-3/MareII).

Since di↵erent samples from these two abortion cases and one semen sample from a stallion from the same abortion outbreak at stud farm 3 were sent for investigation, it is here more clearly representable.

Additionally the abortion isolate P5276 is listed here:

Designation Year Source Sample Genotype Processed ORF 30 REA Sal I Sequencing

character sample nested PCR

Mare I

V09-3-2 2009 stud farm 3 nasal swab EHV-1 DNA positive positive yes

V09-3-12 2009 stud farm 3 lung fluid EHV-1 DNA positive positive yes

V09-3-15 2009 stud farm 3 amniotic fluid EHV-1 DNA positive positive yes Mare II

V09-3-3 2009 stud farm 3 nasal swab EHV-1 DNA positive positive yes

V09-3-23 2009 stud farm 3 lung fluid EHV-1 DNA positive positive yes

Stallion

V09-4-1 2009 stud farm 3 sperm EHV-1 DNA positive positive yes

single abortion case

P5276 not H¨ubert virus isolate EHV-1 DNA positive negative yes

known

115118

Im Dokument Aus dem Institut für Virologie des Fachbereiches Veterinärmedizin. Freien Universität Berlin (Seite 112-119)