Oligonucleotides - Nucleic acid techniques

2. Materials and methods

2.5 Nucleic acid techniques

2.5.5 Oligonucleotides

Oligonucleotide primers used in this work were purchased from MWG-Biotech (Ebersbach, Germany) and are listed in Tab.2.4.

Tab.2.4: Oligonucleotide Primers used in this work

Name Sequence Application

HG 3 GGTCAGGATAGAGAAGGGAGAGC Housekeeping gene: RNA polymerase II large subunit fwd

HG 4 GAAGAAGCCAGTAGTTTACAAGCC Housekeeping gene: RNA polymerase II large subunit rev

HG 5 AGGCGTTCCGTGACACCATC Housekeeping gene: putative APT1A fwd HG 6 CTGGCAACTTCTTCGGCTTCC Housekeeping gene: putative APT1A rev

S53 CCGGGTCGAGATCACCAAGATA TPS2 QRT-PCR

S54 GGGAAGTGAATCAGCAGCCA TPS2 QRT-PCR

S57 GGCAGCACTCCAAGGATCTTCT TPS3 QRT-PCR

S58 TGGGAAGTGAATCAGCAGCCG TPS3 QRT-PCR

AM120 AGGGAACTTCGTGGTGGATGATAC TPS10 QRT-PCR fwd

AM121 TGGCGTCTGGTGAAGGTAATGG TPS10 QRT-PCR rev

BH51 TCTGGATGATGGGAGTCTTCTTTG TPS23 QRT-PCR fwd

BH52 GCGTTGCCTTCCTCTGTGG TPS23 QRT-PCR rev

SF9 CCGACTTAGGCTGCTCATCA ZmOMT1 QRT-PCR fwd

SF10 GCTGCATCTCCCTTGTGTGTC ZmOMT1 QRT-PCR rev

SK10 GATGTTGTGCATAACAGTATTAGGAGC ZmOMT3 QRT-PCR fwd

SK11 ATGCCAAGCGAGATAGTGAGAAAC ZmOMT3 QRT-PCR rev

SM1 CCGACTTAGGCTGCTCATCG ZmOMT4 QRT-PCR fwd

SM2 GCCTCACCCTTGTGGTTTCTT ZmOMT4 QRT-PCR rev

TF3 CCACAGGACAGGACACACAGAG TF1 QRT-PCR fwd

TF4 CTGGATTGCTGGCGATGAACC TF1 QRT-PCR rev

TF5 TGAGCGTGGAGTGGTGGAC TF2 QRT-PCR fwd

TF7 GGGAGGGTGGTGGATGACG TF2 QRT-PCR rev

TF13 AGATGAACAGGAAGCCAGGAGAC TF4 QRT-PCR fwd

TF14 AAGCCACCGCCGAAGGAG TF4 QRT-PCR rev

TF17 ACACCCGCCAGATTTGAAGTAAG TF5 QRT-PCR fwd

TF18 CAGTGGAGTGGATACAGTGATGAG TF5 QRT-PCR rev

TF23 CTACCGCCGCCGTTGTTC TF7 QRT-PCR fwd

TF24 TGCCTTCCTGCTTGCTTGC TF7 QRT-PCR rev

TF25 ATGGAGTTTGACCTGCTGAATTAC TF8 QRT-PCR fwd

TF26 CGGCGTGTACTGCTCTGG TF8 QRT-PCR rev

TF31 CACTACCCGCCCGTCCTC TF9 QRT-PCR fwd

TF32 TGCCGTCGCCCAGAATCG TF9 QRT-PCR rev

TF40 TTCACAGAGGAGGAAGAAGAGC TF12 QRT-PCR fwd

TF41 TGGACGGCGAGGAGGATG TF12 QRT-PCR rev

TF42 ACAGCGATACAAGATTAGCATACG TF13 QRT-PCR fwd

TF43 CAACTACACAAGCAGCGATGG TF13 QRT-PCR rev

TF46 TGCGGTTGGTGGCTCATAAG TF14 QRT-PCR fwd

TF47 AAGGTTCTCAGGCTTCAAGTCC TF14 QRT-PCR rev

TF50 GAAGGGCAAGAAGAAGAAGAAGTC TF15 QRT-PCR fwd

TF51 GGTGAAGCAGGCGGATGG TF15 QRT_PCR rev

TF53 ACTGCTCTGTCCTACTTGGC TF16 QRT-PCR fwd

TF54 AGTCCTCTCGGTCCTTGGC TF16 QRT-PCR rev

TF55 GGAAGCCTTAGTGGAAGCAGTAG TF17 QRT-PCR fwd

TF56 CGTAGTTGATGGAAGCAAGTTGTG TF17 QRT-PCR rev

TF65 GCAGTCTGTCCCATTTGATAGGC TF20 QRT-PCR fwd

TF66 ACTCAGGCTCATAGGTCACTTCC TF20 QRT-PCR rev

TF70 CTCTGGATGATGGATGGCGAAGG TF21 QRT_PCR fwd

TF71 ACAAGGGAAGCACGGCACTG TF21 QRT-PCR rev

TF72 ACTCCCACTCCCAATCACACTC TF22 QRT-PCR fwd

TF73 GCCGTCCACCTGCTCCTC TF22 QRT-PCR rev

TF78 TGGACGGAGATGAATGTGGAGAC TF23 QRT-PCR fwd

TF79 CCCAGGACGACAAGAGGAACC TF23 QRT-PCR rev

TF94 GCCTCGTCTCGTCTCGTCTTC TF28 QRT-PCR fwd

TF95 GGTCAGCCTTCGTCCTCTTCC TF28 QRT-PCR rev

TF96 GCAGAGCAGAAGCCCAGCAG TF29 QRT-PCR fwd

TF97 GGTGGAGATGGTGAGGAGGAAC TF29 QRT-PCR rev

TF100 GACGCTTCCTCACGCCTCTG TF30 QRT-PCR rev

TF101 CCACACCACCTCGCTGAACC TF30 QRT-PCR fwd

TF104 GGCAGCGGTGGAGGAGAC TF31 QRT-PCR fwd

TF105 TCACAAGTTCAGAAGAGCAGAAGC TF31 QRT-PCR rev

TF108 CATCTGCTCCAACCTCTCCTTCTC TF32 QRT-PCR fwd

TF109 GCTGCCCGTCTCCTGAACC TF32 QRT-PCR rev

TF118 CACGCCGCCGGAGGAGTTG TF34 QRT-PCR rev

TF119 GCGCAAGTACGGGCAGAAGCAGG TF34 QRT-PCR fwd

TF122 CGGTGGGCTCAAGACAAAACA TF35 QRT-PCR rev

TF123 TTCCAAAGCAAACAGGCAGAT TF35 QRT-PCR fwd

TF141 TCACTGGGGCAAGTGGGTGG TF40 QRT-PCR fwd

TF142 TTGGCATCGCCTTGCTCTTGG TF40 QRT-PCR rev

TF147 GATGTTGATCACCATGCCGTCC TF42 QRT-PCR fwd

TF148 CAACTCCGCCTCGCAGTGCC TF42 QRT- PCR rev

TF161 TCAAGCTGCCGACCCCTCC TF46 QRT-PCR fwd

TF162 CGCCTCCGTCATAGTGCTTCC TF46 QRT-PCR rev

TF169 ATGACGCCCGGGAACTCCGT TF2 RACE rev

TF170 ACCGCCACCACCACCATCCC TF2 RACE fwd

TF171 GCATACTCCGATCCACGCAGG TF35 RACE fwd

TF172 AAGCATGGGAGTTGGGCATC TF35 RACE rev

TF173 GCTTGAGCTGATGACCACGATG TF8 RACE fwd

TF174 GTACCCGGTGGTGGTGGTCGT TF8 RACE rev

TF175 GGAAGACCCGCACACACACGC TF30 RACE fwd

TF176 CCCATGCCCACGCCTCCGAC TF30 RACE rev

TF177 CAGGTTAAGGGGAGCGAGAAC TF34 RACE fwd

TF178 GGCGCGTGGACAGCGGCTTGG TF34 RACE rev

TF179 ACGGTGAGCAGCACTCTCGCC TF23 RACE fwd

TF180 CCGCGTGGTTTAGCTCCAGTGTTG TF23 RACE rev

TF181 CAGCTGAGCTGATAGTGACCC TF29 RACE fwd

TF182 GGAACCAAAAAAGAAAAGAAAAC TF29 RACE rev

TG1 GTCGACGCATGGGTCCTGAA TF1 cloning fwd

TG2 TCAATGACAGCGCAGAACC TF1 cloning fwd

TG3 CTAAAAAATTCCTCGTTCA TF1 cloning rev

TG4 TGAGCTCACTCAGAAGGG TF1 cloning rev

TG5 AGAAGAATTCAGTTTGGGAG TF2 cloning rev

TG6 TATTCTTGATCATCTCAGCA TF2 cloning rev

TG7 TCGCGTTACATGTCGCCGT TF8 cloning rev

TG8 ATGAGTTTTAGGACTCGTC TF20 cloning fwd

TG9 AAGTAGCATCACATTCCAA TF20 cloning rev

TG10 ATGCAGCTGGGGAAGACCGC TF22 cloning fwd

TG11 CCTCGCCTCAGCTCAGAACT TF22 cloning rev

TG12 GACCTCCCGCCTGGCTGCTCC TF34 cloning fwd

TG13 AGCAGCGGACATGGTAGGAG TF34 cloning fwd

TG14 ATGTCGCCCTCCTCCTACTTCT TF34 cloning fwd

TG15 AGTAGCTGACAGTGGTCGT TF34 cloning fwd

TG16 AGATTGGGTGCGTGCGTCA TF34 cloning rev

TG17 TCGCCAGCTCTAGCAGAGGA TF34 cloning rev

TG18 AAATGCAGATGGACTCCTA TF23 cloning fwd

TG19 ATGCCGGCCTCGTTCCAG TF23 cloning fwd

TG20 GAAGTGGATCAGTCATTGTCA TF23 cloning rev

TG21 TTGTTTGAGTCGACGGGCAT TF35 cloning fwd

TG22 CCTGCCTAGTTCTCTGCC TF30 cloning rev

TG23 CGATCATGCGCTAGCTAGTTCAC TF8 cloning rev

TG24 ATGGGACAGCCGGTGACGAG TF1 cloning fwd

TG25 TCAGAAGGGGCCCAACCCC TF1 cloning rev

TG26 ATGGGTCTGCCGATGAGGAG TF1 cloning fwd

TG27 TCAGAACTGTCCCAACCCG TF1 cloning rev

TG28 ATGCCTTCAGTCGAGGTCG TF2 cloning fwd

TG29 TCAGTTTGGGAGCTCGAGG TF2 cloning rev

TG30 ATGGAGTTTGACCTGCTG TF8 cloning fwd

TG31 TTACATGTCGCCGTGGGG TF8 cloning rev

TG32 ATGTTCCAAGGAATGATGT TF20 cloning fwd

TG33 TCACATTCCAAAGGGGGCA TF20 cloning rev

TG34 ATGGGTCTGCCGATGAGGA TF22 cloning fwd

TG35 TCAGAACTGTCCCAACCC TF22 cloning rev

TG36 ATGCAGATGGACTCCTAC TF23 cloning fwd

TG37 GGAGGATCGCGTGTCAAAAC TF23 cloning fwd

TG38 TCAGTCATTGTCAGCAAAG TF23 cloning rev

TG39 ATGGCGCCCCGGGTGGCGGA TF30 cloning fwd

TG40 CTAGTTCTCTGCCGGCGG TF30 cloning rev

TG41 ATGAGCTGCGCGGGGCAG TF32 cloning fwd

TG42 ATGGTCGACGACCCGGCC TF32 cloning fwd

TG43 CTAGGCGACCGGCGGCCAGT TF32 cloning rev

TG44 ATGGCGTCCTCGACGGGGA TF34 cloning fwd

TG45 TCACCGGTAGAGGGGCGCC TF34 cloning rev

TG46 ATGGACTTCCACCACAGCA TF35 cloning fwd

TG47 TCAAGGGGTGGTGATGTC TF35 cloning rev

TG48 ATGGGTCATGATGAAGCTGTAG TF21 cloning fwd

TG49 GGCACAAGGGAAGCACGGCAC TF21 cloning rev

TH1 TCCGAATTCATGACAGCGCAGAACCC TF1 expression fwd TH2 GCGGCCGCCTAAAAAATTCCTCGTTCACC TF1 expression rev TH3 AGCTTGCGGCCGCCTAAAAAATTCCTCGTTCACC TF1 expression rev TH4 TCCGAAATTCATGCAGCTGGGGAAGACCGCCGTC TF22 expression fwd TH5 TCCGAATTCATGCAGCTGGGGAAGACCGCCGTC TF22 expression fwd TH6 GCGGCCGCTAGATCAGAACTGTCCCAACCC TF22 expression rev TH7 AGCTTGCGGCCGCTAGATCAGAACTGTCCCAACCC TF22 expression rev TH8 TCCGAATTCATGTTCCAAGGAATGATGT TF20 expression fwd TH9 GCGGCCGCAAGTAGCATCACATTCCAAAGGG TF20 expression rev TH10 AGCTTGCGGCCGCAAGTAGCATCACATTCCAAAGGG TF20 expression rev TH11 TCCGAATTCATGAGTTTTAGGACTCGTCATGTTCC TF20 expression fwd

TH12 TCCGAATTCATGAGCTGCGCGGGGCAG TF32 expression fwd

TH13 AGCTTGCGGCCGCCTAGGCGACCGGCGGCCAGT TF32 expression rev

TH14 TCCGAATTCATGGAGTTTGACCTGCTG TF8 expression fwd

TH15 AGCTTGCGGCCGCTTACATGTCGCCGTGGGG TF8 expression rev

TP1 CATCAGCTGCCATGAAAGTATA TPS23 promoter studies 1651-1800 rev TP2 GCTGGCTACCTATATTAAGAAC TPS23 promoter studies 1651-1800fwd TP3 CAAGCACCTGGATCCTATGGT TPS23 promoter studies 1511-1693 rev TP4 TGTGTTGCACATGCCTGTCG TPS23 promoter studies 1511-1693 fwd TP5 GATCTGCCGACAGGCATGTG TPS23 promoter studies 1378-1537 rev TP6 AACATTTGAGGTGTATGGTTG TPSS23 promoter studies 1378-1537 fwd TP7 GCATTTTCATGCATACAACC TPS23 promoter studies 1260-1413 rev TP8 ACGAGTCACGGGTGGCGGTC TPS23 promoter studies 1260-1413 fwd TP9 CATCGATCCTTGCATTAGACC TPS23 promoter studies 1136-1296 rev TP10 CTATACATGTGATCTTTAGAGGT TPS23 promoter studies 1136-1296 fwd TP11 CTCTAAAATGTGAACAATATGTAC TPS23 promoter studies 1026-1180 rev TP12 CAAAGGTTGTATGCACAAAAGGC TPS23 promoter studies 1026-1180 fwd TP13 GAGTATCAAAATCTGATTAATTTC TPS10A promoter studies 8-161 fwd TP14 CAAATGGTCTGATCGGTTAATG TPS10A promoter studies 8-161 rev TP15 AACATACACCGCGTTAGCATC TPS10A promoter studies 115-291 fwd TP16 TGTGTTGATCGTTTTGTCTTG TPS10A promoter studies 115-291 rev TP17 CAAGACAAAACGATCAACACACC TPS10A promoter studies 271-421 fwd TP18 ATGCAATGAACAACCATTCGGG TPS10A promoter studies 271-421 rev

TP19 CATATCCCGAATGGTTGTTC TPS10A promoter studies 395-566 fwd

TP20 GCTGAGACAAAATGGAGTTGC TPS10A promoter studies 395-566 rev TP21 GTCTCAGCTAGGATAAATACTGG TPS10A promoter studies 559-718 fwd TP22 GTACTTCCTCTCAACTATTACTTC TPS10A promoter studies 559-718 rev TP23 GAAGTAATAGTTGAGAGGAAGTACCA TPS10A promoter studies 712-855 fwd TP24 CTTCTGATTGTTTCAGTGTGTTG TPS10A promoter studies 712-855 rev TP25 GAAACAATCAGAAGCTGTAGACG TPS10A promoter studies 842-1005 fwd TP26 CTTGGGTGGAAGGCGGTGGCAT TPS10A promoter studies 842-1005 rev TP27 ACCACAACCTTAATTAATATACACC TPS10B promoter studies 1-174 fwd TP28 AGAATCTCTTAGGAATCTACTTC TPS10B promoter studies 1-174 rev

TP29 AACCATATATATACGGAGCGG TPS10B promoter studies 458- fwd

TP30 ATTACCAGACTTGTGAGCTTGTGG TPS10A promoter studies 622 fwd TPG1 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAAAATTAAT

ACTGGGAAAAGG

TPS23 promoter Gateway cloning1.8 kb TPG2 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTGCAGATGG

AAGAAACACAAC

TPS23 promoter Gateway cloning 600 bp TPG3 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTTAAGCTTG

TAGAATGGCAG

TPS23 promoter Gateway cloning 1.2 kb TPG4 GGGGACCACTTTGTACAAGAAAGCTGGGTCGAAAGTATAG

TATACTAGCTA

TPS23 promoter Gateway cloning rev TPG5 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCTTTGCCTA

GTGTCAAAAAAAACACTC

TPS10 promoter Gateway cloning 1.5 kb TPG6 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCTAGTGTCA TPS10 promoter Gateway cloning 1.5 kb-b

AAAAAAACACTCGGC

TPG7 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAAAGAAGCT CTTTGGCGAGTG

TPS10 promoter Gateway cloning 1.5 kb-c TPG8 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAGTATCAA

AATCTGATTAATTTC

TPS10 promoter Gateway cloning 800 bp TPG9 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCCATTGCATA

ATGTGACTCC

TPS10 promoter Gateway cloning 500 bp TPG10 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAGTAATA

GTTGAGAGGAAGTACCA

TPS10 promoter Gateway cloning 200 bp TPG11 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTGCAGCAGC

CCCTGCACATC

TPS10 promoter Gateway cloning rev TPG12 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTGCACGCAG

AAGTAATAGTTGAGAG

TPS10 promoter Gateway cloning 200 bp new TPG13 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGGATGATGT

AACATTTGAGGTG

TPS23 promoter Gateway cloning 400 bp TPG14 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCAATATAATG

CAAATTGAGTAC

TPS23 promoter Gateway cloning 200 bp

Im Dokument Identification in regulatory factors in the signal transduction pathway in herbivore-induced maize (Seite 40-44)