Molecular replacement

All solutions obtained by molecular replacement were not suitable for further refinement. All solutions obtained by the search for the whole tetramer of RBA1 were rejected due to high R-factors and low accordance with the experimental map.

The solution which was obtained by searching one monomer with the program MOLREP and afterwards placing the missing monomers with the help of the known non crystallographic sym-metry operators (5.2.8Chapter 4.2.8) was rejected as it showed extensive steric clashes.

The same result was obtained when placing manually a monomer in an NCS-averaged map and afterwards placing the missing monomers with help of the known non crystallographic symmetry operators.

This table shows the correlation of the assumed density regions for every monomer to each other

molecule 3 0.848 0.847 0.995 0.848

molecule 4 0.858 0.850 0.846 0.995

molecule 2 0.844 0.995 0.826 0.835

molecule 1 0.995 0.844 0.823 0.843

molecule 1 molecule 2 molecule 3 molecule 4 Table 27 . List of the correlations of the NCS-vectors

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