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6. R EFERENCES

7.6 List of putative transporters in M. pneumoniae

Tab. 7.7 Putative proteins involved in transmembrane transport processes in M. pneumoniae.

MPN # Gene name1,2 Essentiality3 Predicted Function1,2

MPN018 pmd1 F ATP-bind prot.; Multidrug resist. ABC transporter MPN019 msbA NE ATP-bind prot.; Multidrug resist. ABC transporter MPN043 glpF NE Glycerol facilitator

MPN048 NE Membrane export family

MPN049 NE Membrane export familiy

MPN055 potA E ATP-bind. protein for polyamine transport MPN056 potB E Transport permease (potB homolog)

MPN057 potI E Transport permease

MPN058 E Lipoprotein, transport system substrate-binding protein MPN076 NE Hypothetical protein, MFS transporter

MPN077 NE Hypothetical protein, MFS transporter MPN078 fruA F Permease EIIA/B/C component

MPN080 ybbP F ABC transporter membrane protein, FtsX-like permease MPN081 ybbA/ glnQ E ABC transporter ATP-binding protein

MPN095 NE Amino acid permease

MPN096 NE Amino acid permease

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MPN112 NE Permease

MPN113 NE Permease

MPN133 NE Lipoprotein / multiple sugar transport system substrate-binding protein / nuclease / MalE

MPN134 ugpC E Transport system permease, ATP binding protein MPN135 ugpA E Transport system (ABC transporter) permease MPN136 ugpE E Transport system (ABC transporter) permease

MPN193 cysA/cbiO1/ecfA1 F Energy-coupling factor transporter ATP-binding protein MPN194 hisP/cbiO2/ecfA2 E Energy-coupling factor transporter ATP-binding protein

MPN195 cbiQ F Transport protein

MPN207 ptsG E PTS system, glucose-specific EIIABC component MPN209 mgtA / pacL F Probable cation-transporting P-type ATPase MPN215 oppB E Peptide ABC transporter permease

MPN216 amiD / oppC F Peptide ABC transporter permease

MPN217 oppD E Peptide ABC transporter ATP-binding protein MPN218 oppF E Oligopeptide transport system ATP-binding protein,

hypothetical protein

MPN234 NE Membrane export family

MPN258 yjcW/mglA E Simple sugar ABC transporter, ATP-binding protein MPN259 NE Simple sugar ABC transporter permease

MPN260 rbsC F Simple sugar ABC transporter permease

MPN268 F PTS system component EIIB/EIIC domain-containing protein

MPN274 E ABC transporter permease

MPN308 apc NE Amino acid permease (sequence similarity zu mpn095/096)

MPN318 apc F Permease

MPN319 gap1 F permease

MPN333 NE Hypothetical membrane protein

MPN334 bcrA NE ABC transporter ATP binding protein (Multidrug-like transport?)

MPN335 NE Hypothetical membrane protein

MPN415 P37/ phnD F High-affinity transport system substrate-binding protein P37 MPN416 P29/ phnC F ABC transport system ATP-binding protein P29

MPN417 P69/ phnE F ABC transport system permease protein P69

MPN421 NE permease

MPN431 cbiQ E Transporter permease (CbiQ family)

MPN432 artP E Energy-coupling factor transporter ATP-binding protein MPN433 cbiO E Energy-coupling factor transporter ATP-binding protein

MPN435 E Permease

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E essential; F fitness; NE non-essential. 1 Himmelreich et al., 1996; 2 KEGG; 3 LLuch-Senar et al., in press.

MPN448 folT E Hypothetical protein, ECF transporter S component

MPN460 ktrB E KtrB, Trk-type K+ transport systems, membrane components, cation transporter

MPN461 ktrA E KtrA uptake protein, trk system potassium uptake protein TrkA MPN494 yjfU/ulaC (sgaA) F PTS system: ascorbate-specific EIIA component

MPN495 ulaB / sgaB NE PTS system: EIIB

MPN496 yjfU/sgaT/ulaA NE PTS system: ascorbate-specific EIIC component, similar to phosphotransferase protein for pentitol from E.coli, SGAT homolog

MPN508 NE Membrane export protein

MPN509 NE Membrane export protein

MPN510 F Membrane export protein

MPN511 NE Membrane export protein

MPN512 NE Membrane export protein

MPN571 lcnDR3 NE Hemolysin ABC transporter, ABC transporter ATP binding protein

MPN608 phoU / pstU NE Phosphate transport system, regulatory protein MPN609 pstB NE Phosphate import system, ATP-binding protein MPN610 pstA NE Phosphate ABC transport system, permease protein MPN611 pstS NE Phosphate transport system, substrate-binding protein MPN651 mtlA NE Similar to mannitol-specific PTS EIIBC, EIICB-Mtl MPN652 mtlD NE Mannitol-phosphate 5-dehydrogenase: D-mannitol

1-phosphate + NAD+ = D-fructose 6-1-phosphate + NADH MPN653 mtlF NE Mannitol-specific PTS system component EIIA MPN683 devA NE ABC transporter, ATP-binding protein

MPN684 F ABC transporter permease

MPN685 cysA F ABC transporter ATP-binding protein

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Curriculum vitae

STEPHANIE GROßHENNIG Rosenwinkel 22 37081 Göttingen Tel.: 0176/21896140 E-Mail: stgrosshennig@web.de

PERSONALINFORMATION

Name Stephanie Großhennig

Date of birth 04.05.1984 Place of birth Wernigerode

Nationality German

EDUCATION

Since 03/2012 Ph.D. thesis “Novel virulence determinants in Mycoplasma pneumoniae“ in the group of Prof. Dr. Jörg Stülke, Department of General Microbiology, Georg-August University Göttingen

11/2011 Master thesis “Transport systems in Mycoplasma pneumoniae“ in the group of Prof. Dr. Jörg Stülke, Department of General Microbiology, Georg-August University Göttingen

2009-2011 M.Sc. Studies of Microbiology and Biochemistry, Georg-August University Göttingen

08/2009 Bachelor thesis “Control of the intracellular glycerol-3-phosphate level in Mycoplasma pneumoniae – Influence of two homologous phosphodiesterases on the pathogenicity factor H2O2“ in the group of Prof. Dr. Jörg Stülke, Department of General Microbiology, Georg-August University Göttingen 2006-2009 B. Sc. Studies of Biology,

Georg-August University Göttingen

2004-2006 Studies of Landscape architecture, University Kassel 06/2003 Abitur, Heinrich-Heine-Gymnasium Ilsenburg

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INTERNSHIPS

09/2010-11/2010 Lab rotation about “Fascinating Facilitators: Analysis of Transporters in Mycoplasma pneumoniae” in the group of Prof. Dr. Jörg Stülke, Department of General Microbiology, Georg-August University Göttingen

12/2010-02/2011 Lab rotation on „Characterization of Aspergillus fumigatus ΔlaeA, ΔvelB, ΔvelC, and ΔvosA mutants” with Dr. Henriette Irmer,

Department for Molecular Microbiology and Genetics, Georg-August Universität Göttingen

02/2011-04/2011 Internship on “Analysis of listerial GpsB interactions with penicillin-binding proteins (PBPs) and construction of vectors for pbp gene replacement in Listeria monocytogenes” in the group of Dr. Sven Halbedel, FG 11 Bakterielle darmpathogene Erreger und Legionellen,

Robert-Koch-Institut Wernigerode

CONTRIBUTION TO CONFERENCES Presentation

10/2014 Microbiology and Infection2014, Gemeinsame Tagung der VAAM und DGHM, Dresden

Poster

12/2013 GGNB Poster session, Göttingen

3/2013 Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM), Bremen

PUBLICATION

Großhennig S, Schmidl SR, Schmeisky G, Busse J, Stülke J., 2013

Implication of glycerol and phospholipid transporters in Mycoplasma pneumoniae growth and virulence. Infect. Immun. March 2013 vol. 81 no. 3 896-904

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