All units are abbreviated according to the International Unit System.
AA: amino acid
ATP: adenosine 5´-triphosphate BSA: bovine serum albumin CDK: cyclin-dependent kinase
CHAPS: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate DAPI: 4´,6-diamidino-2-phenylindole
DTT: dithiothreitol
ECL: enhanced chemiluminescence EDTA: ethylenedinitrilotetraacetic acid
ELISA: Enzyme Linked Immunosorbent Assay FCS: Fetal calf serum
GFP: green fluorescent protein HCl: hydrochloric acid
HEPES: N-2-Hydroxyethylpiperazine-N`-2-ethane sulfonic acid hMps1: human Monopolar Spindle 1
mAb: monoclonal antibody PBS: Phosphate-buffered saline PCR: Polymerase chain reaction
PIPES: 1,4-Piperazinediethanesulfonic acid PMSF: phenylmethylsulfonyl fluoride
PYT: phosphotyrosine picked threonine kinase RNA: Ribonucleic Acid
RT: room temperature
SDS-PAGE: Sodium dodecylsulfate polyacrylamid gelelectrophoresis siRNA: small interference Ribonucleic Acid
SPB: Spindle Pole Body TTK: T-cell tyrosine kinase WT: wild-type
Table 4: List of primers.
Primer name Primer sequence (5´-3´)
G61 GCA GCC ACT AGT AAC AGG ATT
G62 GCT AAA GCT AAT TGC TTT TGG GAT TGC AAA CC M140 TGC ATC GAT GCC ATG GAA TCC GAG GAT TTA AGT
GGC
M141 GAC ATT ACG AAT AAC TGC AGA TCA TTT TTT TCC CC
Table 5: List of plasmids.
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