3 Materials and Methods .1 Materials
4.4 Influence of breed on lymphocyte subsets distribution
Significant interbreed differences (p < 0.01) are shown in tables 14 and 15. The relative counts of lymphocytes (figure 3) were significantly higher in Beagles and Flat-coated Retrievers than in the other four breeds (p < 0.01). The highest and the lowest absolute counts of lymphocytes (figure 4) were found in Weimaraners and mixed breed dogs, respectively.
Weimaraners showed significantly higher absolute counts of lymphocytes than Bernese mountain dogs, Flat-coated Retrievers, Rottweilers and mixed breed dogs (p < 0.01). Beagles showed significantly higher absolute counts of lymphocytes than mixed breed dogs (p <
0.01).
The highest relative counts of CD4+ T cells (figure 5) were found in Bernese mountain dogs, and the lowest relative counts of CD4+ T cells were found in Weimaraners and Beagles.
Weimaraner and Beagles showed significantly lower relative counts of CD4+ T cells than the other four breeds (p < 0.01), and Bernese mountain dogs showed significantly higher relative counts of CD4+ T cells than Rottweilers (p < 0.01). No significant differences of absolute counts of CD4+ T cells (figure 6) between breeds were found (p > 0.01).
The highest and the lowest relative counts of CD8+ T cells (figure 7) were found in Beagles and Rottweilers, respectively. Beagles and mixed breed dogs showed significantly higher relative counts of CD8+ T cells than Weimaraners and Rottweilers (p < 0.01).
Results
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Rottweilers had significantly lower absolute counts of CD8+ T cells (Figure 8) than Weimaranes, Flat-coated Retrievers, mixed breed dogs and Beagles (p < 0.01), and Beagles showed significantly higher absolute counts of CD8+ T cells than Bernese mountain dogs, Flat-coated Retrievers and mixed breed dogs (p < 0.01).
The highest relative counts of CD3+ T-cells (figure 9) and CD5+ T cells (figure 11) were found in Beagles and Bernese mountain dogs respectively, and the lowest percentages of CD3+ T cells and CD5+ T cells were found in Weimaraners. Weimaraners showed significantly lower relative counts of CD3+ and CD5+ T cells than the other five breeds (p <
0.01). Beagles, mixed breed dogs and Bernese mountain dogs showed significantly higher relative counts of CD3+ T cells than Rottweilers (p < 0.01), and mixed breed dogs and Bernese mountain dogs showed significantly higher relative counts of CD5+ T cells than Rottweilers (p < 0.01). Beagles showed significantly higher absolute counts of CD3+ T cells (figure 10) than Rottweilers and mixed breed dogs (p < 0.01). No significant differences of absolute counts of CD5+ T cells (figure 12) between breeds were found (p > 0.01).
The highest and the lowest relative counts of CD21+ B cells were found in Weimaraners and mixed breed dogs, respectively (figure 13). The relative counts of CD21+ B cells were significantly higher in Weimaraners than in the other five breeds (p < 0.05). Flat-coated Retrievers, Rottweilers and Beagles showed higher relative counts of CD21+ B cells than Bernese mountain dogs and mixed breed dogs (p < 0.01). Weimaraners showed significantly higher absolute counts of CD21+ B-cells (figure 14) than Bernese mountain dogs, Flat-coated Retrievers, Rottweilers and mixed breed dogs (p < 0.05), and Beagles showed significantly higher absolute counts of CD21+ B cells than Bernese mountain dogs and mixed breed dogs (p < 0.01).
Weimaraners had a significantly lower CD4/CD8 ratio (figure 15) than Bernese mountain dogs (p < 0.01).
Results
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Table 14
Relative counts of lymphocyte subsets in the peripheral blood of different dogs breeds. BMD:
Bernese mountain dogs; FCR: Flat-coated Retrievers; MBD: mixed breed dogs.
Parameter BMD Weimaraners FCR Rottweilers MBD Beagles (%) (n = 79) (n = 75) (n = 48) (n = 36) (n = 82) (n = 22) Lymphocyte
median 23c,f 24c,f 32a,b,d,e 24c,f 23c,f 38a,b,d,e (Q1-Q3) (19-31) (20-30) (28-35) (19-28) (19-28) (33-41) CD4+ T cells
median 44b,d,f 32a,c,d,e 39b,f 36a,b,f 40b,f 32a,c,d,e (Q1-Q3) (39-48) (25-37) (35-46) (32-44) (35-45) (28-34) CD8+ T cells
median 20 18e,f 21 17e,f 22b,d 24b,d
(Q1-Q3) (16-25) (15-22) (17-26) (13-21) (18-29) (22-30) CD5+ T cells
median 79b,d 60a,c,d,e,f 75b 72a,b,e 78b,d 70b (Q1-Q3) (73-82) (49-69) (69-78) (60-77) (72-81) (66-75) CD21+B cells
median 11b,c,d,f 24a,c,d,e,f 16a,b,e 18a,b,e 10b,c,d,f 18a,b,e (Q1-Q3) (8-14) (17-36) (11-20) (12-27) (6-16) (16-23) CD3+ T cells
median 78b,d 60a,c,d,e,f 72b 70a,b,e,f 78b,d 82b,d (Q1-Q3) (73-83) (52-68) (68-79) (58-75) (73-81) (75-89) CD4+/CD8+
median 2.2b 1.7a 1.9 2.2 1.9 1.4
(Q1-Q3) (1.7-3) (1.4-2) (1.3-2.5) (1.8-2.9) (1.2-2.5) (1-1.6) a Significant difference versus Bernese mountain dogs.
b Significant difference versus Weimaraners.
c Significant difference versus Flat-coated Retrievers.
d Significant difference versus Rottweilers.
e Significant difference versus mixed breed dogs f Significant difference versus Beagles
Results
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Table 15
Absolute counts of lymphocyte subsets in the peripheral blood of different dogs breeds.
BMD: Bernese mountain dogs; FCR: Flat-coated Retrievers; MBD: mixed breed dogs.
Parameter BMD Weimaraners FCR Rottweilers MBD Beagles (x103/µl) (n =79) (n = 75) (n = 36) (n = 48) (n = 82) (n = 22)
Lymphocyte
median 2.5b 3.5a,c,d,e 2.6b 2.5b 2.3b,f 3.3e (Q1-Q3) (2.0-3.1) (2.5-4.6) (2.1-3.2) (2-3.1) (1.8-2.9) (2.6-4.6)
CD4+ T cells
median 1.1 1 1 0.9 0.9 1
(Q1-Q3) (0.81-1.3) (0.8-1.4) (0.7-1.3) (0.7-1.2) (0.7-1.2) (0.8-1.4)
CD8+ T cells
median 0.5f 0.7d 0.6d,f 0.4b,c,f,e 0.6d,f 0.8a,c,d,e (Q1-Q3) (0.4-0.7) (0.5-0.8) (0.4-0.7) (0.3-0.5) (0.4-0.7) (0.7-1)
CD5+ T cells
Median 2 2 2 1.7 1.8 2.2
(Q1-Q3) (1.6-2.5) (1.5-2.6) (1.5-2.3) (1.4-2.1) (1.4-2.1) (1.8-3.1)
CD21+ B cells
median 0.2b,f 0.8a,c,d,e 0.4b 0.4b 0.2b,f 0.7a,e (Q1-Q3) (0.2-0.4) (0.5-1.3) (0.3-0.6) (0.3-0.7) (0.1-0.4) (0.4-0.8)
CD3+ T cells
median 2 1.8 2 1.7f 1.8f 2.6e,d
(Q1-Q3) (1.6-2.5) (1.5-2.6) (1.6-2.4) (1.3-2.1) (1.4-2.2) (2.3-3.2)
a Significant difference versus Bernese mountain dogs.
b Significant difference versus Weimaraners.
c Significant difference versus Flat-coated Retrievers.
Results
Effect of breed on the relative counts of peripheral blood lymphocytes. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
Lymphocytes % Lymphocytes absolute counts
Figure 4
Effect of breed on the absolute counts of peripheral blood lymphocytes. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
Results
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60
50
40
30
20
10
0
Figure 5
Effect of breed on the relative counts of peripheral blood CD4+ T cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
CD4+ T cells % CD4+ T cells absolute counts
3,5
3,0
2,5
2,0
1,5
1,0
,5 0,0
25%-75%
I Min – Max − Median
25%-75%
I Min – Max − Median BMD BE MBD WM FCR RW
(n=79) (n=22) (n=82) (n=75) (n=48) (n=36) Breed
BMD BE MBD WM FCR RW (n=79) (n=22) (n=82) (n=75) (n=48) (n=36)
Results
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50
40
30
20
10
CD8+ T cells % 0
Figure 7
Effect of breed on the relative counts of peripheral blood CD8+ T cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
1,5
1,0
,5
0,0
CD8+ T cells absolute counts
25%-75%
I Min – Max − Median
25%-75%
I Min – Max − Median BMD BE MBD WM FCR RW
(n=79) (n=22) (n=82) (n=75) (n=48) (n=36) Breed
BMD BE MBD WM FCR RW (n=79) (n=22) (n=82) (n=75) (n=48) (n=36)
Breed
Figure 8
Effect of breed on the absolute counts of peripheral blood CD8+ T cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
Results
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100
80
60
40
20
0
Figure 9
Effect of breed on the relative counts of peripheral blood CD3+ T cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
5
4
3
2
1
CD3+ T cells absolute counts 0
25%-75%
I Min – Max − Median
25%-75%
I Min – Max − Median
BMD BE MBD WM FCR RW (n=79) (n=22) (n=82) (n=75) (n=48) (n=36)
Breed
BMD BE MBD WM FCR RW
CD3+ T cells %
Results
Effect of breed on the relative counts of peripheral blood CD5+ T cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
CD5+ T cells absolute counts
BMD BE MBD
Effect of breed on the absolute counts of peripheral blood CD5+ T cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
Results
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60
40
20
0
Figure 13
Effect of breed on the relative counts of peripheral blood CD21+ B cells. BMD: Bernese mountain dogs; BE: Beagles; MBD: mixed breed dogs; WM: Weimaraners; FCR: Flat-coated Retrievers; RW: Rottweilers.
25%-75%
I Min – Max − Median
6
5
4
3
2
1
0
25%-75%
I Min – Max − Median
CD21+ B cells absolute counts CD21+ B cells %
BMD BE MBD WM FCR RW (n=79) (n=22) (n=82) (n=75) (n=48) (n=36)
Breed
BMD BE MBD WM FCR RW (n=79) (n=22) (n=82) (n=75) (n=48) (n=36)
Results
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8
6