Index of figures - Impact of elicitors on glucosinolate production in plants and exudates of tu

7. ATTACHMENT

7.2 Index of figures

Attachment

Figure Title Page 4.8. Elicitor influence on the kinetic of total glucosinolate content in exudates………. 84 4.9. HPLC profile of glucosinolates recovered from exudates in hydroponic systems

during the first 10days after elicitor application……….. 86 4.10. Elicitors influence on kinetic of aliphatic glucosinolates in exudates………. 90 4.11. Elicitor influence on kinetic of aromatic glucosinolates in exudates of turnip……. 99 4.12. Elicitor influence on kinetic of indole glucosinolate in exudates of turnip……….. 103 4.13. Influence of salicylic acid applied on different stages of plant growth on kinetic

of total glucosinolates in exudates of turnip grown in hydroponic……….. 117 4.14. HPLC profile of glucosinolates recovered from root exudates of H and HSA15

turnip plants on 20^th day……… 118 4.15. Influence of salicylic acid applied on different stages of plant growth on kinetic

of aliphatic glucosinolates in exudates………. 122 4.16. Influence of salicylic acid on kinetic of aromatic glucosinolate content in

exudates………. 130

4.17. Influence of salicylic acid applied on different stages of plant growth on the kinetic of indole glucosinolates in exudates of turnip grown in hydroponic……… 132

Attachment

7. ATTACHMENT 7.3 Supplementary tables

Table 4.1a. Influence of plant nutrition on total glucosinolate content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AD1H 1.5^P^a^P ± 0.2 0.6^P^a^P ± 0.0 ND 0.2^P^a^P ± 0.0 AD2H 1.8^P^ab^P ± 0.3 0.7^P^ab^P ± 0.1 ND 0.2^P^a^P ± 0.1 10 days

AD2H2S 2.0^P^b^P ± 0.1 1.0^P^b^P ± 0.2 ND 0.3^P^a^P ± 0.0 AD1H 2.2^P^a^P ± 0.4 1.6^P^a^P ± 0.2 2.3^P^a^P ± 0.2 0.8^P^a^P ± 0.1 AD2H 3.4^P^b^P ± 0.3 3.8^P^c^P ± 0.1 2.6^P^a^P ± 0.3 1.7^P^b^P ± 0.3 20 days

AD2H2S 2.5^P^a^P ± 0.1 3.3^P^b^P ± 0.4 5.0^P^b^P ± 0.4 1.5^P^b^P ± 0.3 AD1H 3.6^P^a^P ± 0.3 2.8^P^a^P ± 0.2 4.9^P^a^P ± 0.6 1.2^P^a^P ± 0.3 AD2H 3.6^P^a^P ± 0.7 4.5^P^b^P ± 0.8 7.0^P^b^P ± 0.4 2.0^P^a^P ± 0.6 30 days

AD2H2S 3.9^P^a^P ± 0.8 5.6^P^b^P ± 0.7 10.2^P^c^P ± 0.9 4.0^P^c^P ± 0.3

AD1H 2.2^P^a^P ± 0.4*

AD2H 3.6^P^b^P ± 0.2*

Total

AD2H2S 5.9^P^c^P ± 0.6*

AD1H - aeroponic with defensor with Hoagland solution, AD2H - aeroponic with defensor with two time concentrated Hoagland solution, AD2H2S - aeroponic with defensor with two times concentrated Hoagland solution and two times increased concentration of sulfur. The differences are compared for each treatment. Values followed by the same letter are not significantly different. *Sum of glucosinolates exuded during 30 days

Table 4.1b. Influence of plant nutrition on total glucosinolate content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AS1H 0.7^P^a^P ± 0.2 1.0^P^a^P ± 0.2 ND 0.2^P^a^P ± 0.1 AS2H 0.9^P^a^P ± 0.0 0.9^P^a^P ± 0.0 ND 0.2^P^a^P ± 0.1 10 days

AS2H2S 2.1^P^b^P ± 0.2 1.5^P^b^P ± 0.0 ND 0.4^P^a^P ± 0.2 AS1H 2.5^P^a^P ± 0.4 1.4^P^a^P ± 0.2 3.0^P^a^P ± 0.3 1.0^P^a^P ± 0.3 AS2H 3.1^P^a^P ± 0.3 2.1^P^ab^P ± 0.6 5.0^P^b^P ± 0.8 1.4^P^a^P ± 0.2 20 days

AS2H2S 2.7^P^a^P ± 0.5 3.0^P^b^P ± 0.7 6.4^P^b^P ± 0.6 1.4^P^a^P ± 0.4 AS1H 4.9^P^a^P ± 0.7 2.4^P^a^P ± 0.2 8.3^P^a^P ± 0.4 1.2^P^a^P ± 0.0 AS2H 4.6^P^a^P ± 0.3 5.0^P^b^P ± 0.2 9.6^P^b^P ± 0.6 1.6^P^b^P ± 0.3 30 days

AS2H2S 3.9^P^a^P ± 0.4 7.1^P^c^P ± 0.3 10.7^P^c^P ±0.2 3.2^P^c^P ± 0.2

AS1H 2.4^P^a^P ± 0.4*

AS2H 3.1^P^a^P ± 0.3*

Total

AS2H2S 5.0^P^b^P ± 0.4*

AS1H - aeroponic with sprayers with Hoagland solution, AS2H - aeroponic with sprayers with two times concentrated Hoagland solution, AS2H2S - aeroponic with sprayers with two times concentrated Hoagland solution and two times increased concentration of sulfur. The differences are compared for each treatment. Values followed by the same letter are not significantly

Table 4.3a. Influence of plant nutrition on composition of aliphatic, aromatic, and indole glucosinolates in plants and exudates of turnip from aeroponic with defensor on 30^P^th^P day

Object Treatment Aliphatic Aromatic Indole mg plant^P^-1^P 1.6^P^b^P ± 0.4 0.6^P^b^P ± 0.1 1.3^P^a^P ± 0.1 AD1H

% 46 17 37 mg plant^P^-1^P 1.4^P^b^P ± 0.2 0.8^P^b^P ± 0.3 1.4^P^a^P ± 0.1

AD2H

% 39 22 39 mg plant^P^-1^P 2.4^P^c^P ± 0.3 0.1^P^a^P ± 0.0 1.4^P^a^P ± 0.3

Leaves

AD2H2S

% 62 3 36

mg plant^P^-1^P 0.8^P^a^P ± 0.1 0.7^P^a^P ± 0.1 1.3^P^a^P ± 0.3 AD1H

% 29 25 46 mg plant^P^-1^P 0.8^P^a^P ± 0.1 1.0^P^b^P ± 0.1 2.7^P^b^P ± 0.3

AD2H

% 18 22 60 mg plant^P^-1^P 0.9^P^a^P ± 0.2 0.8^P^ab^P ± 0.2 3.1^P^b^P ± 0.3

Secondary roots

AD2H2S

% 19 17 65 mg plant^P^-1^P 2.6^P^a^P ± 0.2 1.3^P^a^P ± 0.3 1.0^P^a^P ± 0.0

AD1H

% 53 27 20 mg plant^P^-1^P 3.7^P^b^P ± 0.3 1.6^P^a^P ± 0.3 1.8^P^b^P ± 0.3

AD2H

% 52 23 25 mg plant^P^-1^P 5.7^P^c^P ± 0.4 2.2^P^b^P ± 0.1 2.9^P^c^P ± 0.2

Primary roots

AD2H2S

% 53 20 27 mg plant^P^-1^P 0.9^P^a^P ± 0.2* 0.6^P^a^P ± 0.2* 0.6^P^a^P ± 0.1*

AD1H

% 43 29 29 mg plant^P^-1^P 1.4^P^b^P ± 0.2* 1.1^P^ab^P ± 0.3* 1.2^P^b^P ± 0.1*

AD2H

% 38 30 32 mg plant^P^-1^P 1.9^P^b^P ± 0.5* 1.3^P^b^P ± 0.1* 2.6^P^c^P ± 0.6*

Exudates

AD2H2S

% 33 22 45

Attachment

Table 4.3b. Influence of plant nutrition on composition of aliphatic, aromatic, and indole glucosinolates in plants and exudates of turnip from aeroponic with sprayers on 30^P^th^P day

Object Treatment Aliphatic Aromatic Indole mg plant^P^-1^P 2.6^P^b^P ± 0.2 0.7^P^b^P ± 0.2 1.6^P^a^P ± 0.1 AS1H

% 53 14 33 mg plant^P^-1^P 2.1^P^a^P ± 0.2 0.6^P^b^P ± 0.2 1.9^P^a^P ± 0.1

AS2H

% 46 13 41 mg plant^P^-1^P 2.6^P^b^P ± 0.4 0.1^P^a^P ± 0.0 1.6^P^a^P ± 0.1

Leaves

AS2H2S

% 60 2 37

mg plant^P^-1^P 0.7^P^a^P ± 0.1 0.7^P^a^P ± 0.2 1.0^P^a^P ± 0.2 AS1H

% 29 29 42 mg plant^P^-1^P 0.8^P^a^P ± 0.1 1.6^P^a^P ± 0.2 2.6^P^b^P ± 0.5

AS2H

% 16 32 52 mg plant^P^-1^P 0.8^P^a^P ± 0.1 1.5^P^a^P ± 0.2 2.5^P^b^P ± 0.5

Secondary roots

AS2H2S

% 17 31 52 mg plant^P^-1^P 3.7^P^a^P ± 0.7 2.6^P^a^P ± 0.2 2.0^P^a^P ± 0.4

AS1H

% 45 31 24 mg plant^P^-1^P 4.0^P^a^P ± 0.3 2.9^P^a^P ± 0.2 2.7^P^ab^P ± 0.7

AS2H

% 42 30 28 mg plant^P^-1^P 5.2^P^b^P ± 0.9 3.7^P^b^P ± 0.3 3.5^P^a^P ± 0.1

Primary roots

AS2H2S

% 42 30 28 mg plant^P^-1^P 1.0^P^a^P ± 0.3* 1.0^P^a^P ± 0.2* 1.0^P^a^P ± 0.2*

AS1H

% 33 33 33 mg plant^P^-1^P 1.0^P^a^P ± 0.2* 1.0^P^a^P ± 0.3* 1.0^P^a^P ± 0.2*

AS2H

% 33 33 33 mg plant^P^-1^P 2.5^P^b^P ± 0.1* 0.8^P^a^P ± 0.1* 1.8^P^b^P ± 0.4*

Exudates

AS2H2S

% 49 16 35

Table 4.4a. Influence of plant nutrition on aliphatic glucosinolate content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AD1H 1.0^P^b^P ± 0.0 0.2^P^a^P ± 0.1 ND 0.1^P^a^P ± 0.1 AD2H 0.9^P^b^P ± 0.2 0.2^P^a^P ± 0.1 ND 0.1^P^a^P ± 0.0 10 days

AD2H2S 1.2^P^b^P ± 0.2 0.2^P^a^P ± 0.1 ND 0.1^P^a^P ± 0.0 AD1H 0.9^P^b^P ± 0.1 0.7^P^b^P ± 0.1 1.3^P^ab^P ± 0.2 0.3^P^a^P ± 0.1 AD2H 1.3^P^b^P ± 0.3 0.4^P^a^P ± 0.1 1.1^P^a^P ± 0.2 0.8^P^b^P ± 0.0 20 days

AD2H2S 1.9^P^a^P ± 0.3 0.5^P^ab^P ± 0.2 1.7^P^b^P ± 0.3 0.7^P^b^P ± 0.2 AD1H 1.6^P^b^P ± 0.4 0.8^P^a^P ± 0.1 2.6^P^a^P ± 0.2 0.5^P^a^P ± 0.1 AD2H 1.4^P^b^P ± 0.2 0.8^P^a^P ± 0.1 3.7^P^b^P ± 0.3 0.5^P^b^P ± 0.0 30 days

AD2H2S 2.4^P^c^P ± 0.3 0.9^P^a^P ± 0.2 5.7^P^c^P ± 0.4 1.1^P^c^P ± 0.1

AD1H 0.9^P^a^P ± 0.2*

AD2H 1.4^P^b^P ± 0.2*

Total

AD2H2S 1.9^P^b^P ± 0.5*

Table 4.4b. Influence of plant nutrition on aliphatic glucosinolate content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AS1H 0.4^P^a^P ± 0.0 0.5^P^b^P ± 0.0 ND 0.1^P^a^P ± 0.0 AS2H 0.4^P^a^P ± 0.0 0.3^P^a^P ± 0.0 ND 0.1^P^a^P ± 0.1 10 days

AS2H2S 1.2^P^b^P ± 0.2 0.3^P^a^P ± 0.0 ND 0.3^P^b^P ± 0.0 AS1H 1.0^P^a^P ± 0.2 0.5^P^a^P ± 0.1 1.2^P^a^P ± 0.2 0.5^P^a^P ± 0.1 AS2H 1.1^P^a^P ± 0.3 0.5^P^a^P ± 0.2 2.2^P^a^P ± 0.1 0.8^P^a^P ± 0.1 20 days

AS2H2S 1.9^P^b^P ± 0.2 0.5^P^a^P ± 0.1 1.9^P^b^P ± 0.4 0.7^P^a^P ± 0.1 AS1H 2.6^P^b^P ± 0.2 0.7^P^a^P ± 0.1 3.7^P^a^P ± 0.7 0.6^P^a^P ± 0.1 AS2H 2.1^P^a^P ± 0.2 0.8^P^a^P ± 0.1 4.0^P^a^P ± 0.3 0.5^P^a^P ± 0.2 30 days

AS2H2S 2.6^P^b^P ± 0.4 0.8^P^a^P ± 0.1 5.2^P^b^P ± 0.9 1.5^P^a^P ± 0.1

AS1H 1.0^P^a^P ± 0.3*

AS2H 1.0^P^a^P ± 0.2*

Total

AS2H2S 2.5^P^b^P ± 0.1*

Attachment

Table 4.5a. Influence of plant nutrition on individual aliphatic glucosinolates content in plants and exudates of turnip from aeroponic with defensor on 30^P^th^P day (mg plant^P^-1^P)

Gluco-sinolates

Treat-ment

Leaves Secondary

roots

Primary roots

Exudates mg plant^P^-1^P 0.4^P^a^P ± 0.1 0.1^P^a^P ± 0.0 0.7^P^a^P ± 0.1 0.2^P^a^P ± 0.0*

AD1H

% 33 8 58 mg plant^P^-1^P 0.5^P^ab^P ± 0.1 0.2^P^ab^P ± 0.1 0.9^P^a^P ± 0.1 0.4^P^b^P ± 0.0*

AD2H

% 31 13 56 mg plant^P^-1^P 0.7^P^b^P ± 0.1 0.3^P^b^P ± 0.1 1.9^P^b^P ± 0.2 0.6^P^c^P ± 0.1*

Gluconapin

AD2H2S

% 24 10 66 mg plant^P^-1^P 0.6^P^a^P ± 0.0 0.4^P^ab^P ± 0.1 0.9^P^a^P ± 0.2 0.4^P^a^P ± 0.1*

AD1H

% 32 21 47 mg plant^P^-1^P 0.6^P^a^P ± 0.1 0.3^P^a^P ± 0.0 1.7^P^b^P ± 0.3 0.5^P^ab^P ± 0.2*

AD2H

% 23 12 65 mg plant^P^-1^P 1.0^P^b^P ± 0.2 0.4^P^b^P ± 0.0 2.2^P^b^P ± 0.4 0.8^P^b^P ± 0.2*

Progoitrin

AD2H2S

% 28 11 61 mg plant^P^-1^P 0.3^P^b^P ± 0.0 0.1^P^a^P ± 0.1 0.4^P^a^P ± 0.1 0.2^P^a^P ± 0.0*

AD1H

% 38 13 50 mg plant^P^-1^P 0.2^P^a^P ± 0.0 0.1^P^a^P ± 0.1 0.5^P^a^P ± 0.1 0.2^P^ab^P ± 0.1*

AD2H

% 25 13 63 mg plant^P^-1^P 0.3^P^ab^P ± 0.1 0.1^P^a^P ± 0.1 0.8^P^b^P ± 0.1 0.3^P^b^P ± 0.1*

Glucobras-sicanapin

AD2H2S

% 25 8 67 mg plant^P^-1^P 0.3^P^b^P ± 0.0 0.2^P^b^P ± 0.0 0.6^P^a^P ± 0.1 0.1^P^a^P ± 0.0*

AD1H

% 27 18 55 mg plant^P^-1^P 0.1^P^a^P ± 0.0 0.1^P^a^P ± 0.0 0.6^P^a^P ± 0.2 0.3^P^b^P ± 0.0*

AD2H

% 13 13 75 mg plant^P^-1^P 0.6^P^c^P ± 0.2 0.2^P^ab^P ± 0.1 0.8^P^b^P ± 0.1 0.2^P^ab^P ± 0.1*

Gluconapo-leiferin

AD2H2S

% 38 13 50

Table 4.5b. Influence of plant nutrition on individual aliphatic glucosinolates content in plants and exudates of turnip from aeroponic with sprayers on 30^P^th^P day (mg plant^P^-1^P)

Gluco-sinolates

Treat-ment

Leaves Secondary

roots

Primary roots

Exudates mg plant^P^-1^P 0.6^P^a^P ± 0.0 0.3^P^b^P ± 0.0 0.9^P^a^P ± 0.2 ^0.1^P^a^P^{± 0.0*}

AS1H

% 33 17 50 mg plant^P^-1^P 0.6^P^a^P ± 0.1 0.2^P^a^P ± 0.0 1.0^P^a^P ± 0.2 ^0.2^P^b^P^{± 0.0*}

AS2H

% 33 11 56 mg plant^P^-1^P 0.6^P^a^P ± 0.1 0.2^P^ab^P ± 0.1 1.7^P^b^P ± 0.2 ^0.9^P^c^P^{± 0.1*}

Gluconapin

AS2H2S

% 24 8 68 mg plant^P^-1^P 1.1^P^a^P ± 0.2 0.2^P^a^P ± 0.0 1.3^P^a^P ± 0.2 ^0.4^P^a^P^{± 0.1*}

AS1H

% 42 8 50 mg plant^P^-1^P 0.9^P^a^P ± 0.2 0.4^P^b^P ± 0.1 1.8^P^b^P ± 0.2 ^0.4^P^a^P^{± 0.1*}

AS2H

% 29 13 58 mg plant^P^-1^P 1.2^P^a^P ± 0.2 0.4^P^b^P ± 0.1 2.0^P^b^P ± 0.2 ^1.0^P^b^P^{± 0.2*}

Progoitrin

AS2H2S

% 33 11 56 mg plant^P^-1^P 0.5^P^b^P ± 0.1 0.1^P^a^P ± 0.0 0.6^P^a^P ± 0.1 ^0.2^P^a^P^{± 0.0*}

AS1H

% 38 8 54 mg plant^P^-1^P 0.2^P^a^P ± 0.0 0.1^P^a^P ± 0.0 0.7^P^a^P ± 0.1 ^0.2^P^a^P^{± 0.0*}

AS2H

% 20 10 70 mg plant^P^-1^P 0.4^P^b^P ± 0.1 0.2^P^a^P ± 0.1 0.6^P^a^P ± 0.1 ^0.3^P^a^P^{± 0.1*}

Glucobras-sicanapin

AS2H2S

% 33 17 50 mg plant^P^-1^P 0.4^P^a^P ± 0.1 0.2^P^b^P ± 0.0 0.7^P^a^P ± 0.2 ^0.1^P^a^P^{± 0.0*}

AS1H

% 31 15 54 mg plant^P^-1^P 0.4^P^a^P ± 0.1 0.1^P^a^P ± 0.0 0.5^P^a^P ± 0.1 ^0.2^P^b^P^{± 0.0*}

AS2H

% 40 10 50 mg plant^P^-1^P 0.5^P^a^P ± 0.2 0.1^P^a^P ± 0.0 0.7^P^a^P ± 0.1 ^0.2^P^ab^P^{± 0.1*}

Gluconapo-leiferin

AS2H2S

% 38 8 54

Attachment

Table 4.8a. Individual indole glucosinolates in plants and exudates from aeroponic with defensor on 30^P^th^P day (mg plant^P^-1^P)

Glucosinolates Treatment Leaves Secondary roots

Primary roots

Exudates AD1H 0.4^P^a^P ± 0.0 0.1^P^a^P± 0.0 0.2^P^a^P ± 0.0 0.2^P^a^P ± 0.1 AD2H 0.5^P^a^P ± 0.1 0.8^P^b^P± 0.2 0.6^P^b^P ± 0.1 0.4^P^b^P ± 0.0 Glucobrassicin

AD2H2S 0.9^P^b^P ± 0.1 1.1^P^b^P± 0.2 0.8^P^b^P ± 0.1 0.8^P^c^P ± 0.3 AD1H 0.2^P^a^P ± 0.1 0.4^P^a^P± 0.1 0.2^P^a^P ± 0.0 0.2^P^ab^P ± 0.1 AD2H 0.6^P^b^P ± 0.1 0.8^P^b^P± 0.2 0.4^P^b^P ± 0.1 0.3^P^b^P ± 0.0

4-hydroxy-glucobrassicin

AD2H2S 0.5^P^b^P ± 0.0 0.3^P^a^P ± 0.1 0.5^P^b^P ± 0.1 0.1^P^a^P ± 0.1 AD1H 0.4^P^b^P ± 0.0 0.1^P^ab^P± 0.1 0.2^P^a^P ± 0.0 0.1^P^a^P ± 0.0 AD2H 0.2^P^a^P ± 0.1 0.1^P^a^P± 0.0 0.2^P^a^P ± 0.0 0.2^P^b^P ± 0.0

4-methoxy-glucobrassicin

AD2H2S 0.1^P^a^P ± 0.0 0.2^P^b^P ± 0.0 0.6^P^b^P ± 0.2 0.5^P^c^P ± 0.1 AD1H 0.3^P^b^P ± 0.1 0.7^P^a^P± 0.2 0.4^P^a^P ± 0.1 0.1^P^a^P ± 0.1 AD2H 0.1^P^ab^P ± 0.1 1.0^P^ab^P± 0.2 0.5^P^a^P ± 0.2 0.3^P^b^P ± 0.0 Neoglucobrassicin

AD2H2S 0.1^P^a^P ± 0.0 1.2^P^b^P ± 0.2 1.4^P^b^P ± 0.2 1.2^P^c^P ± 0.2

AD1H - aeroponic with defensor with Hoagland solution, AD2H - aeroponic with defensor with two times concentrated Hoagland solution, AD2H2S - aeroponic with defensor with two times concentrated Hoagland solution and two times increased concentration of sulfur. The differences are compared for each treatment. Values followed by the same letter are not significantly different.

Table 4.8b. Individual indole glucosinolates in plants and exudates from aeroponic with sprayers on 30^P^th^P day (mg plant^P^-1^P)

Glucosinolates Treatment Leaves Secondary roots

Primary roots

Exudates AD1H 0.4^P^a^P ± 0.1 0.3^P^a^P± 0.0 0.6^P^a^P± 0.1 0.3^P^b^P ± 0.0 AD2H 0.6^P^a^P ± 0.2 0.6^P^b^P± 0.1 0.8^P^a^P± 0.1 0.1^P^a^P ± 0.0 Glucobrassicin

AD2H2S 0.6^P^a^P ± 0.1 0.7^P^b^P± 0.1 0.9^P^a^P± 0.2 0.4^P^b^P ± 0.2 AD1H 0.5^P^a^P ± 0.1 0.3^P^a^P± 0.1 0.5^P^a^P± 0.0 0.2^P^a^P ± 0.1 AD2H 0.7^P^a^P ± 0.2 0.8^P^b^P± 0.2 0.7^P^b^P± 0.2 0.2^P^a^P ± 0.2

4-hydroxy-glucobrassicin

AD2H2S 0.7^P^a^P ± 0.2 0.4^P^a^P ± 0.1 0.5^P^ab^P ± 0.2 0.1^P^a^P ± 0.2 AD1H 0.2^P^ab^P ± 0.1 0.3^P^a^P± 0.0 0.4^P^a^P± 0.0 0.2^P^a^P ± 0.2 AD2H 0.2^P^b^P ± 0.0 0.5^P^b^P± 0.1 0.7^P^b^P± 0.2 0.2^P^a^P ± 0.1

4-methoxy-glucobrassicin

AD2H2S 0.1^P^a^P ± 0.0 0.5^P^ab^P ± 0.2 0.9^P^b^P ± 0.3 0.5^P^a^P ± 0.2 AD1H 0.4^P^a^P ± 0.2 0.4^P^a^P± 0.1 0.5^P^a^P± 0.2 0.4^P^a^P ± 0.2 AD2H 0.4^P^a^P ± 0.1 0.7^P^ab^P± 0.2 0.5^P^a^P± 0.1 0.5^P^a^P ± 0.1 Neoglucobrassicin

AD2H2S 0.4^P^a^P ± 0.1 0.9^P^b^P ± 0.2 1.3^P^b^P ± 0.2 0.9^P^b^P ± 0.2

Table 4.11a. Influence of plant nutrition on gluconasturtiin content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AD1H 0.2^P^a^P ± 0.0 0.2^P^a^P ± 0.0 ND 0.1^P^a^P ± 0.0 AD2H 0.3^P^b^P ± 0.0 0.3^P^b^P ± 0.0 ND 0.1^P^a^P ± 0.0 10 days

AD2H2S 0.2^P^ab^P ± 0.2 0.2^P^ab^P ± 0.1 ND 0.1^P^a^P ± 0.1 AD1H 0.5^P^a^P ± 0.1 0.2^P^a^P ± 0.0 0.6^P^a^P ± 0.0 0.2^P^a^P ± 0.0 AD2H 0.9^P^b^P ± 0.0 1.2^P^b^P ± 0.4 1.0^P^b^P ± 0.0 0.3^P^a^P ± 0.1 20 days

AD2H2S 0.2^P^a^P ± 0.0 0.9^P^b^P ± 0.2 1.6^P^c^P ± 0.2 0.2^P^a^P ± 0.0 AD1H 0.6^P^b^P ± 0.1 0.7^P^a^P ± 0.1 1.3^P^a^P ± 0.3 0.3^P^a^P ± 0.1 AD2H 0.8^P^b^P ± 0.3 1.0^P^b^P ± 0.1 1.6^P^a^P ± 0.3 0.7^P^b^P ± 0.2 30 days

AD2H2S 0.1^P^a^P ± 0.0 0.8^P^ab^P ± 0.2 2.2^P^b^P ± 0.1 1.0^P^b^P ± 0.1

AD1H 0.6^P^a^P ± 0.2*

AD2H 1.1^P^ab^P ± 0.3*

Total

AD2H2S 1.3^P^b^P ± 0.1*

Table 4.11b. Influence of plant nutrition on gluconasturtiin content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AS1H 0.1^P^a^P ± 0.0 0.3^P^a^P ± 0.2 ND ND AS2H 0.2^P^ab^P ± 0.1 0.3^P^a^P ± 0.2 ND 0.1^P^a^P ± 0.0 10 days

AS2H2S 0.2^P^b^P ± 0.0 0.7^P^a^P ± 0.1 ND ND AS1H 0.7^P^ab^P ± 0.3 0.5^P^a^P ± 0.2 1.1^P^a^P ± 0.2 0.2^P^a^P ± 0.0 AS2H 0.8^P^b^P ± 0.1 0.7^P^a^P ± 0.2 1.7^P^b^P ± 0.2 0.3^P^ab^P ± 0.1 20 days

AS2H2S 0.4^P^a^P ± 0.0 1.3^P^a^P ± 0.2 2.2^P^c^P ± 0.2 0.3^P^b^P± 0.0 AS1H 0.7^P^b^P ± 0.2 0.7^P^a^P ± 0.2 2.6^P^a^P ± 0.2 0.2^P^a^P ± 0.1 AS2H 0.6^P^b^P ± 0.2 1.6^P^a^P ± 0.2 2.9^P^a^P ± 0.2 0.4^P^ab^P ± 0.2 30 days

AS2H2S 0.1^P^a^P ± 0.0 1.5^P^a^P ± 0.2 3.7^P^b^P ± 0.3 0.5^P^b^P ± 0.0

AS1H 1.0^P^a^P ± 0.2*

AS2H 1.0^P^a^P ± 0.3*

Total

AS2H2S 0.8^P^a^P ± 0.1*

Attachment

Table 4.12a. Influence of plant nutrition on indole glucosinolate content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary

roots Primary roots Exudates AD1H 0.3^P^a^P ± 0.1 0.2^P^a^P ± 0.0 ND ND AD2H 0.6^P^b^P ± 0.1 0.3^P^a^P ± 0.1 ND ND 10 days

AD2H2S 0.2^P^a^P ± 0.1 1.0^P^b^P ± 0.2 ND 0.1^P^a^P ± 0.0 AD1H 0.8^P^a^P ± 0.1 0.7^P^a^P ± 0.3 0.4^P^a^P ± 0.0 0.2^P^a^P ± 0.1 AD2H 1.2^P^b^P ± 0.1 2.2^P^b^P ± 0.3 0.5^P^a^P ± 0.2 0.3^P^a^P ± 0.1 20 days

AD2H2S 0.9^P^a^P ± 0.1 2.4^P^b^P ± 0.3 1.3^P^b^P ± 0.0 0.6^P^b^P ± 0.1 AD1H 1.3^P^a^P ± 0.1 1.3^P^a^P ± 0.3 1.0^P^a^P ± 0.0 0.4^P^a^P ± 0.1 AD2H 1.4^P^a^P ± 0.1 2.7^P^b^P ± 0.3 1.8^P^b^P ± 0.3 0.9^P^b^P ± 0.1 30 days

AD2H2S 1.4^P^a^P ± 0.3 3.1^P^b^P ± 0.3 2.9^P^c^P ± 0.2 1.8^P^c^P ± 0.3

AD1H 0.6^P^a^P ± 0.1*

AD2H 1.2^P^b^P ± 0.1*

Total

AD2H2S 2.6^P^c^P ± 0.6*

Table 4.12a. Influence of plant nutrition on indole glucosinolate content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots

Primary roots Exudates AS1H 0.2^P^a^P ± 0.1 0.3^P^a^P ± 0.0 ND ND AS2H 0.2^P^a^P ± 0.1 0.3^P^a^P ± 0.0 ND ND 10 days

AS2H2S 0.3^P^a^P ± 0.0 1.0^P^b^P ± 0.2 ND 0.2^P^a^P ± 0.0 AS1H 0.8^P^a^P ± 0.1 0.5^P^a^P ± 0.0 0.6^P^a^P ± 0.1 0.3^P^a^P ± 0.1 AS2H 1.2^P^a^P ± 0.1 0.9^P^b^P ± 0.1 1.1^P^b^P ± 0.0 0.2^P^a^P ± 0.1 20 days

AS2H2S 0.8^P^a^P ± 0.1 2.4^P^c^P ± 0.1 0.8^P^a^P ± 0.1 0.5^P^a^P ± 0.2 AS1H 1.6^P^a^P ± 0.1 1.0^P^a^P ± 0.2 2.0^P^a^P ± 0.4 0.4^P^a^P ± 0.1 AS2H 1.9^P^a^P ± 0.1 2.6^P^b^P ± 0.5 2.7^P^ab^P ± 0.7 0.7^P^ab^P ± 0.2 30 days

AS2H2S 1.6^P^a^P ± 0.1 2.5^P^b^P ± 0.5 3.5^P^a^P ± 0.1 1.1^P^b^P ± 0.3

AS1H 1.0^P^a^P ± 0.2*

AS2H 1.0^P^a^P ± 0.2*

Total

AS2H2S 1.8^P^b^P ± 0.4*

Table 4.18a. Influence of plant nutrition on on fresh weight (g) and glucosinolate concentration (mg g^P^-1^P) in turnip plants from aeroponic with defensor

Roots Time

Treat-ment

Fresh weight, g/

concentration of glucosinolates,

mg g^P^-1^P

Leaves

secondary primary Leaf :

root index g 5.7^P^b^P ± 0.7 1.3^P^c^P ± 0.4 4.4 AD1H

mg g^P^-1^P 0.2 0.2

g 5.4^P^b^P ± 0.7 1.8^P^c^P ± 0.4 3.0 AD2H

mg g^P^-1^P 0.2 0.4

g 8.8^P^b^P ± 0.5 2.8^P^c^P ± 0.0 3.1 10 days

AD2H2S

mg g^P^-1^P 0.2 0.1

g 17.2^P^b^P ± 1.4 8.6^P^c^P ± 0.3 2.4^P^a^P ± 0.9 2.0 AD1H

mg g^P^-1^P 0.3 0.9 0.4

g 17.9^P^b^P ± 1.3 9.6^P^c^P ± 1.0 2.3^P^a^P ± 0.9 1.9 AD2H

mg g^P^-1^P 0.2 1 0.6

g 24.5^P^b^P ± 1.6 12.4^P^b^P ± 1.3 1.6^P^a^P ± 0.1 2.0 20 days

AD2H2S

mg g^P^-1^P 0.1 1 0.4

g 20.2^P^b^P ± 0.8 12.7^P^c^P ± 1.9 4.4^P^a^P ± 0.3 1.6 AD1H

mg g^P^-1^P 0.2 1.1 0.2

g 27.1^P^b^P ± 1.3 18.3^P^c^P ± 2.8 5.2^P^a^P ± 0.2 1.5 AD2H

mg g^P^-1^P 0.2 1.2 0.2

g 29.7^P^b^P ± 1.2 21.1^P^a^P ± 2.1 5.7^P^b^P ± 0.1 1.3 30 days

AD2H2S

mg g^P^-1^P 0.2 1.4 0.3

Attachment

Table 4.18b. Influence of plant nutrition on on fresh weight (g) and glucosinolate concentration (mg g^P^-1^P) in turnip plants from aeroponic with sprayers

Roots Time

Treat-ment Fresh weight, g/

concentration of glucosinolates,

mg g^P^-1^P

Leaves

secondary primary Leaf :

root index g 13.2^P^a^P ± 0.9 4.7^P^a^P ± 0.2 2.8 AS1H

mg g^P^-1^P 0.2 0.2

g 13.8^P^a^P ± 0.7 3.3^P^a^P ± 0.4 4.1 AS2H

mg g^P^-1^P 0.2 0.4

g 13.7^P^a^P ± 1.3 3.0^P^a^P ± 0.4 4.3 10 days

AS2H2S

mg g^P^-1^P 0.2 0.1

g 18.9^P^a^P ± 0.8 6.1^P^a^P ± 0.7 3.6^P^a^P ± 0.2 3.1 AS1H

mg g^P^-1^P 0.3 0.9 0.4

g 20.4^P^a^P ± 1.9 9.0^P^a^P ± 0.4 3.3^P^a^P ± 0.2 2.3 AS2H

mg g^P^-1^P 0.2 1 0.6

g 27.7^P^a^P ± 2.8 13.5^P^a^P ± 0.7 4.2^P^a^P ± 0.8 2.0 20 days

AS2H2S

mg g^P^-1^P 0.1 1 0.4

g 32.9^P^a^P ± 3.4 14.4^P^a^P ± 0.6 7.6^P^a^P ± 0.4 2.3 AS1H

mg g^P^-1^P 0.2 1.1 0.2

g 31.0^P^a^P ± 1.7 18.7^P^a^P ± 0.3 7.0^P^a^P ± 0.3 1.6 AS2H

mg g^P^-1^P 0.2 1.2 0.2

g 36.6^P^a^P ± 2.8 21.9^P^a^P ± 1.1 9.2^P^a^P ± 0.3 1.6 30 days

AS2H2S

mg g^P^-1^P 0.2 1.4 0.3

Table 4.19a. Elicitor influence on total glucosinolate content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots Primary roots Exudates AD 2.0^P^a^P ± 0.1 1.0^P^a^P ± 0.2 0.3^P^a^P ± 0.0 ADSA^B₀^B 3.0^P^b^P ± 0.2 2.0^P^b^P ± 0.4 1.4^P^b^P ± 0.2 10 days

ADMJ^B₀^B 3.6^P^b^P ± 0.7 2.5^P^b^P ± 0.2 1.8^P^b^P ± 0.2 AD 2.1^P^a^P ± 0.5 1.8^P^a^P ± 0.3 3.0^P^b^P ± 0.3 0.7^P^a^P ± 0.2 ADSA^B₀^B 3.3^P^b^P ± 0.4 3.8^P^b^P ± 0.6 3.8^P^b^P ± 0.8 1.9^P^b^P ± 0.1 15 days

ADMJ^B₀^B 6.0^P^b^P ± 0.5 5.5^P^b^P ± 1.2 2.0^P^a^P ± 0.1 2.0^P^b^P ± 0.3 AD 2.5^P^a^P ± 0.1 3.4^P^a^P ± 0.4 5.0^P^c^P ± 0.4 0.8^P^a^P ± 0.1 ADSA^B₀^B 3.7^P^b^P ± 0.6 6.4^P^b^P ± 0.5 4.3^P^b^P ± 0.1 1.5^P^b^P ± 0.1 20 days

ADMJ^B₀^B 6.2^P^c^P ± 0.2 7.4^P^c^P ± 0.3 2.6^P^a^P ± 0.1 1.3^P^c^P ± 0.2 AD 3.0^P^a^P ± 0.1 5.0^P^a^P ± 0.3 8.4^P^ab^P ± 0.7 1.9^P^b^P ± 0.2 ADSA^B₀^B 4.6^P^b^P ± 0.5 8.8^P^b^P ± 0.9 6.8^P^b^P ± 0.3 1.1^P^a^P ± 0.3 25 days

ADMJ^B₀^B 6.8^P^b^P ± 0.4 8.3^P^b^P ± 0.7 7.5^P^a^P ± 0.5 1.2^P^a^P ± 0.4 AD 3.9^P^a^P ± 0.8 5.6^P^a^P ± 0.7 10.2^P^a^P ± 0.9 2.1^P^c^P ± 0.3 ADSA^B₀^B 4.6^P^c^P ± 0.4 10.8^P^c^P ± 1.3 8.6^P^a^P ± 0.7 1.5^P^b^P ± 0.1 30 days

ADMJ^B₀^B 5.9^P^b^P ± 0.4 7.6^P^b^P ± 0.4 9.0^P^a^P ± 1.1 1.0^P^a^P ± 0.1

AD 5.9^P^a^P ± 0.5*

ADSA^B₀^B 7.4^P^b^P ± 0.4*

Total

ADMJ^B₀^B 7.3^P^b^P ± 0.5*

AD – aeroponic with defensor, two times concentrated Hoagland solution + two times increased sulfur; ADSA^B0^B - aeroponic with defensor, two times concentrated Hoagland solution + two times increased sulfur + salicylic acid applied on the beginning;

ADMJ^B₀^B - aeroponic with defensor, two times concentrated Hoagland solution + two times increased sulfur + methyl jasmonate applied on the beginning. The differences are compared for each treatment. Values followed by the same letter are not significantly different. *Sum of glucosinolates exuded during 30 days

Table 4.19a. Elicitor influence on total glucosinolate content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots Primary roots Exudates AS 2.1^P^a^P ± 0.2 1.5^P^a^P ± 0.0 0.4^P^a^P ± 0.2 10 days

ASSA^B₀^B 2.8^P^b^P ± 0.4 2.0^P^b^P ± 0.2 1.4^P^b^P ± 0.3 AS 2.1^P^a^P ± 0.1 1.8^P^a^P ± 0.1 5.5^P^a^P ± 1.0 0.6^P^a^P ± 0.3 15 days

ASSA^B₀^B 4.2^P^b^P ± 0.3 5.9^P^b^P ± 0.6 4.0^P^a^P ± 0.7 1.6^P^b^P ± 0.2 AS 2.7^P^a^P ± 0.5 3.0^P^a^P ± 0.7 6.4^P^b^P ± 0.6 0.8^P^a^P ± 0.1 20 days

ASSA^B₀^B 4.6^P^b^P ± 0.6 7.1^P^b^P ± 0.9 4.7^P^a^P ± 0.6 1.3^P^b^P ± 0.1 AS 3.4^P^a^P ± 0.5 5.3^P^a^P ± 0.6 8.7^P^a^P ± 1.2 1.2^P^a^P ± 0.1 25 days

ASSA^B₀^B 4.6^P^a^P ± 0.6 8.4^P^b^P ± 0.4 6.8^P^a^P ± 1.0 1.3^P^a^P ± 0.1 AS 3.9^P^a^P ± 0.4 7.1^P^a^P ± 0.3 10.7^P^b^P ±0.2 2.0^P^b^P ± 0.2 30 days

ASSA^B0^B 5.1^P^b^P ± 0.4 10.8^P^b^P ± 0.5 8.6^P^a^P ± 0.7 1.3^P^a^P ± 0.2

AS 5.0^P^a^P ± 0.4*

Total

ASSA^B0^B 6.9^P^b^P ± 0.4*

Attachment Table 4.20a. Elicitor influence on composition of aliphatic, aromatic, and indole glucosinolates in plants and exudates of turnip in aeroponic with defensor on 30^P^th^P day

Treatment Aliphatic Aromatic Indole

mg plant^P^-1^P 2.6^P^c^P ± 0.1 ND 1.3^P^a^P ± 0.1 AD

% 67 0 33

mg plant^P^-1^P 1.8^P^a^P ± 0.1 0.4^P^a^P ± 0.2 1.9^P^b^P ± 0.1 ADSA^B0^B

% 44 10 46 mg plant^P^-1^P 2.2^P^b^P ± 0.2 0.6^P^a^P ± 0.2 2.8^P^c^P ± 0.2

Leaves

ADMJ^B0^B

% 39 11 50 mg plant^P^-1^P 1.3^P^a^P ± 0.2 1.2^P^a^P ± 0.4 2.3^P^a^P ± 0.2

% 27 25 48 mg plant^P^-1^P 1.5^P^a^P ± 0.3 2.3^P^b^P ± 0.4 6.3^P^c^P ± 0.5

ADSA^B0^B

15 23 62 mg plant^P^-1^P 1.1^P^a^P ± 0.3 2.8^P^b^P ± 0.3 3.9^P^b^P ± 0.2

Secondary roots

ADMJ^B0^B

% 14 36 50 mg plant^P^-1^P 6.8^P^c^P ± 0.2 0.7^P^a^P ± 0.2 1.4^P^a^P ± 0.2

% 76 8 16

mg plant^P^-1^P 3.5^P^a^P ± 0.4 3.4^P^b^P ± 0.3 1.2^P^a^P ± 0.2 ADSA^B0^B

% 43 42 15 mg plant^P^-1^P 4.9^P^b^P ± 0.4 3.8^P^b^P ± 0.4 1.1^P^a^P ± 0.3

Primary roots

ADMJ^B0^B

% 50 39 11 mg plant^P^-1^P 1.9^P^a^P ± 0.5* 1.3^P^a^P ± 0.1* 2.6^P^a^P ± 0.6*

% 33 22 45 mg plant^P^-1^P 1.5^P^a^P ± 0.4* 2.2^P^b^P ± 0.2* 3.7^P^b^P ± 0.1*

ADSA^B0^B

% 20 30 50 mg plant^P^-1^P 1.3^P^a^P ± 0.4* 1.9^P^b^P ± 0.4* 4.2^P^b^P ± 0.8*

Exudates

ADMJ^B0^B

% 18 26 57

ADMJ^B0^B - aeroponic with defensor, two times concentrated Hoagland solution + two times increased sulfur + methyl jasmonate applied on the beginning. The differences are compared for each treatment. Values followed by the same letter are not significantly different. ND – not determined. *Sum of glucosinolates exuded during 30 days

Table 4.20b. Elicitor influence on composition of aliphatic, aromatic, and indole glucosinolates in plants and exudates of turnip in aeroponic with sprayers on 30^P^th^P day

Treatment Aliphatic Aromatic Indole

mg plant^P^-1^P 2.6^P^c^P ± 0.1 ND 1.3^P^a^P ± 0.1 AS

% 67 0 33

mg plant^P^-1^P 1.8^P^a^P ± 0.1 0.4^P^a^P ± 0.2 1.9^P^b^P ± 0.1 Leaves

ASSA^B0^B

% 44 10 46 mg plant^P^-1^P 1.3^P^a^P ± 0.2 1.2^P^a^P ± 0.4 2.3^P^a^P ± 0.2

% 27 25 48 mg plant^P^-1^P 1.5^P^a^P ± 0.3 2.3^P^b^P ± 0.4 6.3^P^c^P ± 0.5

Secondary roots

ASSA^B0^B

15 23 62 mg plant^P^-1^P 6.8^P^c^P ± 0.2 0.7^P^a^P ± 0.2 1.4^P^a^P ± 0.2

% 76 8 16

mg plant^P^-1^P 3.5^P^a^P ± 0.4 3.4^P^b^P ± 0.3 1.2^P^a^P ± 0.2 Primary

roots

ASSA^B0^B

% 43 42 15 mg plant^P^-1^P 2.4^P^a^P ± 0.1* 0.8^P^b^P ± 0.1* 1.8^P^a^P ± 0.2*

% 48 16 36 mg plant^P^-1^P 2.7^P^a^P ± 0.2* 1.7^P^b^P ± 0.1* 2.5^P^b^P ± 0.2*

Exudates

ASSA^B0^B

% 39 25 36

AS – aeroponic with sprayers, two times concentrated Hoagland solution + two times increased sulfur; ASSA^B₀^B - aeroponic with sprayers, two times concentrated Hoagland solution + two times increased sulfur + salicylic acid applied on the beginning. The differences are compared for each treatment. Values followed by the same letter are not significantly different. ND – not determined. *Sum of glucosinolates exuded during 30 days

Attachment Table 4.21a. Elicitor influence on aliphatic glucosinolate content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary

roots

Primary roots Exudates AD 1.2^P^b^P ± 0.2 0.2^P^ab^P ± 0.1 0.1^P^a^P ± 0.0 ADSA^B0^B 0.8^P^a^P ± 0.1 0.2^P^a^P ± 0.0 0.3^P^c^P ± 0.0 10 days

ADMJ^B0^B 1.5^P^b^P ± 0.3 0.4^P^b^P ± 0.2 0.2^P^b^P ± 0.0 AD 1.6^P^a^P ± 0.4 0.4^P^ab^P ± 0.1 1.0^P^b^P ± 0.0 0.3^P^a^P ± 0.0 ADSA^B₀^B 1.6^P^a^P ± 0.2 0.3^P^a^P ± 0.1 1.0^P^a^P ± 0.4 0.3^P^a^P ± 0.1 15 days

ADMJ^B0^B 2.1^P^a^P ± 0.4 0.7^P^b^P ± 0.2 0.7^P^ab^P ± 0.2 0.3^P^a^P ± 0.1 AD 1.6^P^a^P ± 0.3 0.5^P^a^P ± 0.2 1.7^P^b^P ± 0.2 0.4^P^a^P ± 0.2 ADSA^B₀^B 1.6^P^a^P ± 0.2 0.8^P^ab^P ± 0.1 0.9^P^a^P ± 0.1 0.3^P^a^P ± 0.1 20 days

ADMJ^B₀^B 2.1^P^a^P ± 0.4 0.9^P^b^P ± 0.1 1.2^P^a^P ± 0.3 0.3^P^a^P ± 0.2 AD 1.8^P^a^P ± 0.3 0.7^P^a^P ± 0.2 3.5^P^a^P ± 0.2 0.5^P^b^P ± 0.2 ADSA^B₀^B 1.7^P^a^P ± 0.2 1.1^P^a^P ± 0.1 3.2^P^a^P ± 0.2 0.3^P^ab^P ± 0.2 25 days

ADMJ^B₀^B 2.0^P^a^P ± 0.4 0.9^P^ab^P ± 0.2 3.8^P^a^P ± 0.3 0.2^P^a^P ± 0.0 AD 2.7^P^c^P ± 0.3 0.9^P^a^P ± 0.2 5.7^P^c^P ± 0.4 0.6^P^a^P ± 0.1 ADSA^B₀^B 1.9^P^a^P ± 0.4 1.3^P^b^P ± 0.1 3.5^P^a^P ± 0.2 0.4^P^a^P ± 0.2 30 days

ADMJ^B₀^B 2.1^P^ab^P ± 0.5 1.2^P^ab^P ± 0.1 3.9^P^b^P ± 0.2 0.3^P^a^P ± 0.2

AD 1.9^P^a^P ± 0.5*

ADSA^B0^B 1.5^P^a^P ± 0.4*

Total

ADMJ^B₀^B 1.3^P^a^P ± 0.4*

Table 4.21b. Elicitor influence on aliphatic glucosinolate content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary

roots

Primary roots Exudates AS 1.2^P^b^P ± 0.2 0.3^P^b^P ± 0.0 0.3^P^a^P ± 0.0 10 days

ASSA^B₀^B 0.6^P^a^P ± 0.2 0.1^P^a^P ± 0.1 0.4^P^a^P ± 0.2 AS 1.6^P^a^P ± 0.3 0.4^P^a^P ± 0.2 1.0^P^a^P ± 0.0 0.3^P^a^P ± 0.1 15 days

ASSA^B₀^B 1.4^P^a^P ± 0.2 0.6^P^a^P ± 0.1 1.0^P^a^P ± 0.2 0.4^P^a^P ± 0.1 AS 1.9^P^b^P ± 0.2 0.5^P^a^P ± 0.1 1.9^P^a^P ± 0.4 0.4^P^a^P ± 0.1 20 days

ASSA^B0^B 1.5^P^a^P ± 0.1 0.8^P^b^P ± 0.0 1.5^P^a^P ± 0.5 0.5^P^a^P ± 0.1 AS 2.1^P^a^P ± 0.5 0.7^P^a^P ± 0.2 3.8^P^b^P ± 0.4 0.6^P^a^P ± 0.0 25 days

ASSA^B₀^B 1.7^P^a^P ± 0.3 0.9^P^a^P ± 0.2 1.9^P^a^P ± 0.3 0.6^P^a^P ± 0.2 AS 2.6^P^b^P ± 0.4 0.8^P^a^P ± 0.1 5.2^P^b^P ± 0.9 0.9^P^a^P ± 0.1 30 days

ASSA^B₀^B 1.7^P^a^P ± 0.3 1.1^P^a^P ± 0.5 3.9^P^a^P ± 0.3 0.8^P^a^P ± 0.2

AS 2.4^P^a^P ± 0.1*

Total

ASSA^B₀^B 2.7^P^a^P ± 0.2*

Table 4.22a. Elicitor influence on individual aliphatic glucosinolates content in plants and exudates of turnip from aeroponic with defensor on 30^P^th^P day (mg plant^P^-1^P)

Gluco-sinolates

Treat-ment

Leaves Secondary

roots

Primary roots

Exudates mg plant^P^-1^P 0.6^P^c^P ± 0.1 0.1^P^a^P ± 0.0 1.7^P^b^P ± 0.3 0.5^P^a^P ± 0.2*

% 25 4 71 mg plant^P^-1^P 0.1^P^a^P ± 0.0 0.1^P^a^P ± 0.0 0.6^P^b^P ± 0.1 0.4^P^a^P ± 0.1*

ADSA^B0^B

% 13 13 75 mg plant^P^-1^P 0.4^P^b^P ± 0.0 0.2^P^b^P± 0.0 0.2^P^a^P ± 0.1 0.4^P^a^P ± 0.1*

Gluconapin

ADMJ^B0^B

% 50 25 25 mg plant^P^-1^P 0.9^P^a^P ± 0.1 0.4^P^a^P ± 0.1 1.9^P^a^P ± 0.2 0.9^P^b^P ± 0.2*

% 28 13 59 mg plant^P^-1^P 0.8^P^a^P ± 0.2 0.4^P^a^P ± 0.1 1.8^P^a^P ± 0.1 0.6^P^b^P ± 0.1*

ADSA^B0^B

% 27 13 60 mg plant^P^-1^P 0.7^P^a^P ± 0.1 0.4^P^a^P ± 0.0 1.5^P^a^P± 0.2 0.4^P^ab^P ± 0.2*

Progoitrin

ADMJ^B0^B

% 27 15 58 mg plant^P^-1^P 0.2^P^b^P ± 0.0 0.1^P^a^P ± 0.1 1.0^P^a^P ± 0.3 0.2^P^a^P ± 0.1*

% 15 8 77 mg plant^P^-1^P 0.2^P^b^P ± 0.0 0.5^P^b^P ± 0.1 0.7^P^a^P ± 0.2 0.2^P^a^P ± 0.0*

ADSA^B0^B

% 14 36 50 mg plant^P^-1^P 0.1^P^a^P ± 0.0 0.1^P^a^P ± 0.0 0.8^P^a^P ± 0.2 0.2^P^a^P ± 0.1*

Glucobras-sicanapin

ADMJ^B0^B

% 10 10 80 mg plant^P^-1^P 1.0^P^a^P ± 0.2 0.3^P^a^P ± 0.1 1.0^P^b^P ± 0.2 0.3^P^a^P ± 0.1*

% 43 13 43 mg plant^P^-1^P 0.8^P^a^P ±0.2 0.3^P^a^P ± 0.1 0.4^P^a^P ± 0.1 0.3^P^a^P ± 0.0*

ADSA^B0^B

% 53 20 27 mg plant^P^-1^P 1.0^P^a^P ± 0.2 0.4^P^a^P ± 0.2 1.4^P^c^P± 0.1 0.3^P^a^P ± 0.1*

Gluconapo-leiferin

ADMJ^B0^B

% 36 14 50

Attachment

Table 4.22b. Elicitor influence on individual aliphatic glucosinolates content in plants and exudates of turnip from aeroponic with sprayers on 30^P^th^P day (mg plant^P^-1^P)

Gluco-sinolates

Treat-ment Leaves Secondary

roots Primary

roots Exudates mg plant^P^-1^P 0.6^P^b^P ± 0.1 0.2^P^a^P ± 0.1 1.7^P^a^P ± 0.2 0.9^P^a^P ± 0.2*

% 24 8 68 mg plant^P^-1^P 0.3^P^a^P ± 0.0 0.5^P^b^P ± 0.1 1.4^P^a^P ± 0.3 1.3^P^a^P ± 0.3*

Gluconapin

ASSA^B0^B

% 14 23 64 mg plant^P^-1^P 1.2^P^b^P ± 0.2 0.4^P^a^P ± 0.1 2.0^P^b^P ± 0.2 1.0^P^a^P ± 0.2*

% 33 11 56 mg plant^P^-1^P 0.6^P^a^P ± 0.1 0.3^P^a^P ± 0.2 1.2^P^a^P ± 0.2 0.8^P^a^P ± 0.2*

Progoitrin

ASSA^B0^B

% 29 14 57 mg plant^P^-1^P 0.4^P^a^P ± 0.1 0.2^P^a^P ± 0.1 0.6^P^a^P ± 0.1 0.3^P^a^P ± 0.1*

% 33 17 50 mg plant^P^-1^P 0.4^P^a^P ± 0.1 0.1^P^a^P ± 0.2 0.6^P^a^P ± 0.3 0.5^P^a^P ± 0.1*

Glucobras-sicanapin

ASSA^B0^B

% 36 9 55 mg plant^P^-1^P 0.5^P^a^P ± 0.2 0.1^P^a^P ± 0.0 1.0^P^a^P ± 0.1 0.2^P^a^P ± 0.1*

% 31 6 63 mg plant^P^-1^P 0.4^P^a^P ± 0.1 0.2^P^b^P ± 0.0 0.7^P^a^P ± 0.1 0.2^P^a^P ± 0.0*

Gluconapo-leiferin

ASSA^B0^B

% 31 15 54

Table 4.28a. Elicitor influence on aromatic glucosinolate content in plants and exudates of turnip from aeroponic with defensor (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots Primary roots Exudates AD 0.2^P^a^P ± 0.2 0.2^P^a^P ± 0.1 0.1^P^a^P ± 0.1 ADSA^B₀^B 0.3^P^a^P ± 0.1 0.5^P^b^P ± 0.1 0.4^P^b^P ± 0.1 10 days

ADMJ^B₀^B 0.1^P^a^P ± 0.1 0.3^P^ab^P ± 0.1 0.2^P^ab^P ± 0.1 AD 0.1^P^a^P ± 0.1 0.3^P^a^P ± 0.1 0.7^P^b^P ± 0.1 0.1^P^a^P ± 0.1 ADSA^B₀^B 0.4^P^b^P ± 0.1 0.6^P^b^P ± 0.1 1.5^P^b^P ± 0.2 0.5^P^b^P ± 0.1 15 days

ADMJ^B0^B 0.3^P^ab^P ± 0.2 1.1^P^c^P ± 0.2 1.2^P^a^P ± 0.2 0.5^P^b^P ± 0.0 AD 0.2^P^a^P ± 0.0 0.9^P^a^P ± 0.2 1.6^P^b^P ± 0.2 0.1^P^a^P ± 0.0 ADSA^B₀^B 0.3^P^a^P ± 0.1 1.5^P^b^P ± 0.3 2.0^P^b^P ± 0.1 0.5^P^b^P ± 0.2 20 days

ADMJ^B₀^B 0.2^P^a^P ± 0.0 1.4^P^b^P ± 0.2 1.3^P^a^P ± 0.1 0.4^P^b^P ± 0.0 AD 0.2^P^a^P± 0.1 1.3^P^a^P± 0.0 2.2^P^ab^P± 0.4 0.5^P^a^P± 0.0 ADSA^B₀^B 0.2^P^ab^P± 0.2 2.1^P^b^P± 0.4 2.6^P^b^P± 0.4 0.4^P^a^P± 0.1 25 days

ADMJ^B₀^B 0.4^P^b^P ± 0.1 1.9^P^b^P ± 0.2 2.3^P^a^P ± 0.2 0.6^P^a^P ± 0.2 AD 0.1^P^a^P ± 0.2 0.8^P^a^P ± 0.2 2.2^P^a^P ± 0.1 0.5^P^a^P ± 0.1 ADSA^B₀^B 0.3^P^ab^P ± 0.0 2.2^P^b^P ± 0.1 2.8^P^b^P ± 0.1 0.4^P^a^P ± 0.1 30 days

ADMJ^B₀^B 0.5^P^b^P ± 0.1 1.9^P^b^P ± 0.2 2.6^P^b^P ± 0.3 0.3^P^a^P ± 0.1

AD 1.3^P^a^P ± 0.1*

ADSA^B0^B 2.2^P^b^P ± 0.2*

Total

ADMJ^B₀^B 1.9^P^b^P ± 0.4*

AD – aeroponic with defensor, two times concentrated Hoagland solution + two times increased sulfur; ADSA^B₀^B - aeroponic with defensor, two times concentrated Hoagland solution + two times increased sulfur + salicylic acid applied on the beginning;

Table 4.28b. Elicitor influence on aromatic glucosinolate content in plants and exudates of turnip from aeroponic with sprayers (mg plant^P^-1^P)

Date Treatment Leaves Secondary roots Primary roots Exudates AS 0.2^P^a^P ± 0.0 0.7^P^a^P ± 0.1 ND 10 days

ASSA^B0^B 0.4^P^b^P ± 0.0 1.2^P^b^P ± 0.1 0.4^P^a^P ± 0.1 AS 0.3^P^a^P ± 0.2 0.9^P^a^P ± 0.2 1.1^P^a^P ± 0.1 0.1^P^a^P ± 0.0 15 days

ASSA^B₀^B 0.5^P^b^P ± 0.1 1.9^P^b^P ± 0.3 1.9^P^b^P ± 0.0 0.6^P^b^P ± 0.1 AS 0.4^P^a^P ± 0.0 1.3^P^a^P ± 0.2 2.2^P^a^P ± 0.2 0.2^P^a^P ± 0.0 20 days

ASSA^B₀^B 0.5^P^a^P ± 0.2 2.4^P^b^P ± 0.2 2.3^P^a^P ± 0.1 0.3^P^a^P ± 0.1 AS 0.5^P^a^P ± 0.2 1.5^P^a^P ± 0.2 2.7^P^a^P ± 0.2 0.2^P^a^P ± 0.1 25 days

ASSA^B₀^B 0.5^P^a^P ± 0.1 2.5^P^b^P ± 0.1 2.5^P^a^P ± 0.2 0.3^P^a^P ± 0.1 AS 0.1^P^a^P ± 0.0 1.5^P^a^P ± 0.2 3.7^P^b^P ± 0.3 0.3^P^a^P ± 0.0 30 days

ASSA^B0^B 0.4^P^b^P ± 0.2 2.5^P^b^P ± 0.2 2.8^P^a^P ± 0.2 0.2^P^a^P ± 0.0

AS 0.8^P^b^P ± 0.1*

30 days

ASSA^B₀^B 1.7^P^b^P ± 0.1*

Im Dokument Impact of elicitors on glucosinolate production in plants and exudates of turnip (Brassica rapa) (Seite 172-197)