4. CAN DWARFED OILSEED RAPE ( BRASSICA NAPUS L.) MEASURE UP TO TALL
4.4 D ISCUSSION
4.4.3 Implications for transgenic mitigation
In conclusion, semi-dwarf hybrids did not clearly profit from a fitness advantage when confronted with stressors important for feral plants on low-quality ruderal soils. As the tall cultivar showed a superior performance in some situations, transgenic mitigation through dwarfing may reduce gene flow via ruderal populations. Yet, it needs to be stressed that semi-dwarfed hybrids still produced seeds under all conditions, and sometimes matched the yield of the tall cultivar. Even dwarfed varieties will thus contribute to gene flow via ruderal sites, especially those of high soil quality, enabling transgene escape to conventional fields and hybridisation partners. I thus consider the method of transgenic mitigation through dwarfing as insufficient for an effective limitation of transgene escape.
References
Al-Ahmad, H., Dwyer, J., Moloney, M. & Gressel, J. (2006) Mitigation of establishment of Brassica napus transgenes in volunteers using a tandem construct containing a selectively unfit gene. Plant Biotechnology Journal, 4, 7–21.
Angadi, S. V. & Entz, M. H. (2002) Water Relations of Standard Height and Dwarf Sunflower Cultivars. Crop Science, 42, 152–159.
Armesto, J. J. & Pickett, S. T. A. (1985) Experiments on Disturbance in Old-Field Plant Communities:
Impact on Species Richness and Abundance. Ecology, 66, 230–240.
Arora, A. & Mohan, J. (2001) Expression of Dwarfing Genes under Nitrogen and Moisture Stress in Wheat (Triticum spp): Dry Matter Partitioning, Root Growth and Leaf Nitrogen. Journal of Agronomy and Crop Science, 186, 111–118.
Beckie, H. J. & Warwick, S. I. (2010) Persistence of an oilseed rape transgene in the environment.
Crop Protection, 29, 509–512.
Blum, A. & Sullivan, C. (1997) The Effect of Plant Size on Wheat Response to Agents of Drought Stress. I. Root Drying. Australian Journal of Plant Physiology, 24, 35-41.
Boege, K. (2005) Influence of plant ontogeny on compensation to leaf damage. American Journal of Botany, 92, 1632–1640.
Breckling, B. & Menzel, G. (2004) Self-organised pattern in oilseed rape distribution - An issue to be considered in risk analysis. Risk Hazard Damage. Specification of Criteria to Assess Environmental Impact of Genetically Modified Organisms (eds B. Breckling & R. Verhoeven), pp.
73–88, Bonn.
Byington, T., Gottschalk, K. & McGraw, J. (1994) Within-population Variation in Response of Red Oak Seedlings to Herbivory by Gypsy Moth Larvae. American Midland Naturalist, 132, 328–339.
Chapman, M. A. & Burke, J. M. (2006) Letting the gene out of the bottle: the population genetics of genetically modified crops. New Phytologist, 170, 429–443.
Cramer, N. (1990) Raps. Züchtung - Anbau und Vermarktung von Körnerraps. Ulmer, Stuttgart.
Crawley, M. J. (1983) Herbivory. The Dynamics of Animal-Plant Interactions. Blackwell Scientific Publications, Oxford.
Crawley, M. J. & Brown, S. L. (1995) Seed Limitation and the Dynamics of Feral Oilseed Rape on the M25 Motorway. Proceedings of the Royal Society of London B, 259, 49–54.
Devos, Y., Reheul, D., Schrijver, A. de, Cors, F. & Moens, W. (2004) Management of herbicide-tolerant oilseed rape in Europe: a case study on minimizing vertical gene flow. Environmental Biosafety Research, 3, 135–148.
D'Hertefeldt, T., Jorgensen, R. B. & Pettersson, L. B. (2008) Long-term persistence of GM oilseed rape in the seedbank. Biology Letters, 4, 314–317.
Duffy, G., Grégoire, S. & Martinelli, A. (2007) Validation of Tetrazolium method for Brassica spp.
ISTA Method Validation Reports, 4. The International Seed Testing Association (ISTA), Bassersdorf.
Elling, B., Neuffer, B. & Bleeker, W. (2009) Sources of genetic diversity in feral oilseed rape (Brassica napus) populations. Basic and Applied Ecology, 10, 544–553.
Fargue, A., Meynard, J. M., Colbach, N., Vallee, P., Grandeau, G. & Renard, M. (2004) Contamination of rapeseed harvest by volunteers of other varieties: a study of intergenotypic competition. European Journal of Agronomy, 21, 193–207.
Gavloski, J. E. & Lamb, R. J. (2000) Compensation by Cruciferous Plants is Specific to the Type of Simulated Herbivory. Environmental Entomology, 29, 1273–1282.
Gressel, J. (1999) Tandem constructs: preventing the rise of superweeds. Trends in Biotechnology, 17, 361–366.
Gressel, J. (2005) Crop Ferality and Volunteerism. Taylor & Francis Group, Boca Raton, Florida.
Hails, R. S. (2000) Genetically modified plants - the debate continues. Trends in Ecology & Evolution, 15, 14–18.
Hall, L., Topinka, K., Huffman, J., Davis, L. & Good, A. (2000) Pollen flow between herbicide-resistant Brassica napus is the cause of multiple-herbicide-resistant B. napus volunteers. Weed Science, 48, 688–694.
Hendrix, S. D. (1979) Compensatory reproduction in a biennial herb following insect defloration.
Oecologia, 42, 107–118.
Hilbert, D. W., Swift, D. M., Detling, J. K. & Dyer, M. I. (1981) Relative growth rates and the grazing optimization hypothesis. Oecologia, 51, 14–18.
Hochwender, C. G., Marquis, R. J. & Stowe, K. A. (2000) The potential for and constraints on the evolution of compensatory ability in Asclepias syriaca. Oecologia, 122, 361–370.
Huapeng, L., Yun, W., Xiaocheng, L., Yong, G., Zhijun, W., Yun, Z. & Maolin, W. (2011) A GA-insensitive dwarf mutant of Brassica napus L. correlated with mutation in pyrimidine box in the promoter of GID1. Molecular Biology Reports, 38, 191–197.
Huhta, A.-P. (2001) Restorative mowing on semi-natural grasslands: community-level changes and species-level responses. Dissertation, University of Oulu, Oulu.
Huhta, A.-P., Lennartsson, T., Tuomi, J., Rautio, P. & Laine, K. (2000) Tolerance of Gentianella campestris in relation to damage intensity: an interplay between apical dominance and herbivory.
Evolutionary Ecology, 14, 373–392.
IBM (2013) Testing Homogeneity of Covariance Matrices. http://publib.boulder.ibm.com/infocenter/
spssstat/v20r0m0/index.jsp?topic=%2Fcom.ibm.spss.statistics.cs%2Fglmm_patlos_homcov.htm (19.07.13).
Islam, N. & Evans, E. J. (1994) Influence of lodging and nitrogen rate on the yield and yield attributes of oilseed rape (Brassica napus L.). TAG Theoretical and Applied Genetics, 88, 530–534.
Jørgensen, R. B., Hauser, T., D’Hertefeldt, T., Andersen, N. & Hooftman, D. (2009) The variability of processes involved in transgene dispersal—case studies from Brassica and related genera.
Environmental Science and Pollution Research, 16, 389–395.
Kawata, M., Murakami, K. & Ishikawa, T. (2009) Dispersal and persistence of genetically modified oilseed rape around Japanese harbors. Environmental Science and Pollution Research, 16, 120–
126.
Khan, R., Rashid, A., Khan, M. & Khan, M. S. (2007) Impact of topping rapeseed (Brassica napus L.) on the economic returns under Rodh Kohi conditions. Pakistan Journal of Biological Sciences, 10, 2740–2743.
Kirkham, M. & Smith, E. (1978) Water Relations of Tall and Short Cultivars of Winter Wheat. Crop Science, 18, 227–230.
Klimešová, J., Janeček, Š., Bartušková, A., Lanta, V. & Doležal, J. (2010) How is Regeneration of Plants after Mowing Affected by Shoot Size in Two Species-Rich Meadows with Different Water Supply? Folia Geobotanica, 45, 225–238.
Klüßendorf-Feiffer, A. (2009) Druscheignung als zentrale Führungsgröße im Erntemanagement am Beispiel von vier verfahrenstechnischen Ansätzen, Dissertation, Humboldt-University Berlin, Berlin.
Koch, R. & Bruins, J. (2008) Höstraps - nya hybridsorter. Framtidsmöjligheter för saväl livsmedel som bioenergi. http://194.47.52.113/janlars/partnerskapAlnarp/ekonf/20080117/Bruins.pdf (24.04.2012).
Lafitte, H. R., Li, Z. K., Vijayakumar, C. H. M., Gao, Y. M., Shi, Y., Xu, J. L., Fu, B. Y., Yu, S. B., Ali, A. J., Domingo, J., Maghirang, R., Torres, R. & Mackill, D. (2006) Improvement of rice drought tolerance through backcross breeding: Evaluation of donors and selection in drought nurseries. Preparing Rice for a Water-Limited Future: from Molecular to Regional Scale.International Rice Research Congress. Field Crops Research, 97, 77–86.
Legere, A. (2005) Risks and consequences of gene flow from herbicide-resistant crops: canola (Brassica napus L) as a case study. Pest Management Science, 61, 292–300.
Lennartsson, T., Nilsson, P. & Tuomi, J. (1998) Induction of Overcompensation in the Field Gentian, Gentianella campestris. Ecology, 79, 1061–1072.
LWK Niedersachsen (2009) Landessortenversuche der Landwirtschaftskammer Niedersachsen.
Winterraps 2009. http://www.lwk-niedersachsen.de/download.cfm/file/327,f55ee0ff-237d-eebf-5ef596dc88cab51f~pdf.html (06.11.2013)
Marquis, R. J. (1984) Leaf Herbivores Decrease Fitness of a Tropical Plant. Science, 226, 537–539.
Maschinski, J. & Whitham, T. (1989) The Continuum of Plant Responses to Herbivory: The Influence of Plant Association, Nutrient Availability, and Timing. American Naturalist, 134, 1–19.
McNaughton, S.J. (1979) Grazing as an Optimization Process: Grass-Ungulate Relationships in the Serengeti. The American Naturalist, 113, 691–703.
McNaughton, S. J. (1983) Compensatory Plant Growth as a Response to Herbivory. Oikos, 40, 329–
336.
Menzel, G. (2006) Verbreitungsdynamik und Auskreuzungspotenzial von Brassica napus L. (Raps) im Goßraum Bremen. Basiserhebung zum Monitoring von Umweltwirkungen transgener Kulturpflanzen. Dissertation, University of Bremen, GCA-Verlag, Waabs.
Muangprom, A., Mauriera, I. & Osborn, T. (2006) Transfer of a Dwarf Gene from Brassica rapa to Oilseed B. napus, Effects on Agronomic Traits, and Development of a ‘Perfect’ Marker for Selection. Molecular Breeding, 17, 101–110.
Nagarajan, S., Rane, J., Maheswari, M. & Gambhir, P. N. (1999) Effect of Post-Anthesis Water Stress on Accumulation of Dry Matter, Carbon and Nitrogen and Their Partitioning in Wheat Varieties Differing in Drought Tolerance. Journal of Agronomy and Crop Science, 183, 129–136.
Nowatzki, T. & Weiss, M. (1997) Effects of simulated and flea beetle injury to cotyledons on growth of drought-stressed oilseed rape. Canadian Journal of Plant Science, 77, 475–481.
Oesterheld, M. & McNaughton, S. J. (1988) Intraspecific Variation in the Response of Themeda triandra to Defoliation: The Effect of Time of Recovery and Growth Rates on Compensatory Growth. Oecologia, 77, 181–186.
Paige, K. N. & Whitham, T. G. (1987) Overcompensation in Response to Mammalian Herbivory: The Advantage of Being Eaten. The American Naturalist, 129, 407–416.
Pessel, F. D., Lecomte, J., Emeriau, V., Krouti, M., Messean, A. & Gouyon, P. H. (2001) Persistence of oilseed rape (Brassica napus L.) outside of cultivated fields. Theoretical and Applied Genetics, 102, 841–846.
Pilson, D. & Decker, K. L. (2002) Compensation for Herbivory in Wild Sunflower: Response to Simulated Damage by the Head-Clipping Weevil. Ecology, 83, 3097–3107.
Phillips, I. D. J. (1971) Effect of relative hormone concentration on auxin-gibberellin interaction in correlative inhibition of axillary buds. Planta, 96, 27-34.
Quinn, G. P. & Keough, M. J. (2007) Experimental design and data analysis for biologists. Cambridge Univiversity Press, Cambridge.
Rapool Ring GmbH (2007) Spätsaat. http://www.rapool.de/printview.cfm/id/312/print/yes.html (30.11.2007).
Renard, M., Delourme, R., Barret, P., Brunel, D., Froger, N. & Tanguy, X. (2010) Mutant gene of the GRAS family and plants with reduced development containing said mutant gene. United States Patent No. US 7,645,920 B1 (24.04.2012).
Reuter, H., Menzel, G., Pehlke, H. & Breckling, B. (2008) Hazard mitigation or mitigation hazard?
Environmental Science and Pollution Research, 15, 529–535.
Ricciardi, L. & Stelluti, M. (1995) The response of durum wheat cultivars and Rht1/rht1 near-isogenic lines to simulated photosynthetic stresses. Journal of Genetics and Breeding, 49, 365–374.
Rose, C., Millwood, R., Moon, H., Rao, M., Halfhill, M., Raymer, P., Warwick, S., Al-Ahmad, H., Gressel, J. & Stewart, C. N. (2009) Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) x Brassica napus (oilseed rape) hybrid populations. BMC Biotechnology, 9, 93.
Schafer, M. G., Ross, A. A., Londo, J. P., Burdick, C. A., Lee, E. H., Travers, S. E., van de Water, P.
K. & Sagers, C. L. (2011) The Establishment of Genetically Engineered Canola Populations in the U.S. PLoS ONE, 6, e25736.
Schoenenberger, N. & D'Andrea, L. (2012) Surveying the Occurrence of subspontaneous glyphosate-tolerant genetically engineered Brassica napus L. (Brassicaceae) along Swiss railways.
Environmental Sciences Europe, 24, 23.
Senior, I. J. & Dale, P. J. (2002) Herbicide-tolerant crops in agriculture: oilseed rape as a case study.
Plant Breeding, 121, 97–107.
Sheppard, S. & Bates, T. (1980) Yield and chemical composition of rape in response to nitrogen, phosphorous and potassium. Canadian Journal of Soil Science, 60, 152–162.
Siddique, K. H. M., Belford, R. K. & Tennant, D. (1990) Root:shoot ratios of old and modern, tall and semi-dwarf wheats in a mediterranean environment. Plant and Soil, 121, 89–98.
Sieling, K. & Kage, H. (2008) The potential of semi-dwarf oilseed rape genotypes to reduce the risk of N leaching. Journal of Agricultural Science, 146, 77–84.
Singh, V. P. & Arora, A. (2001) Intraspecific Variation in Nitrogen Uptake and Nitrogen Utilization Efficiency in Wheat (Triticum aestivum L.). Journal of Agronomy and Crop Science, 186, 239–
244.
Sokal, R. & Rohlf, J. (1969) Biometry. The principles and practice of statistics in biological research.
W.H. Freeman and Company, New York.
Squire, G., Breckling, B., Dietz Pfeilstetter, A., Jørgensen, R. B., Lecomte, J., Pivard, S., Reuter, H. &
Young, M. (2010) Status of feral oilseed rape in Europe: its minor role as a GM impurity and its potential as a reservoir of transgene persistence. Environmental Science and Pollution Research, 18, 111–115.
Stowe, K. A., Marquis, R. J., Hochwender, C. G. & Simms, E. L. (2000) The Evolutionary Ecology of Tolerance to Consumer Damage. Annual Review of Ecology and Systematics, 31, 565–595.
Susko, D. & Superfisky, B. (2009) A comparison of artificial defoliation techniques using canola (Brassica napus). Plant Ecology, 202, 169–175.
Sutherland, J. P., Justinova, L. & Poppy, G. M. (2006) The responses of crop – wild Brassica.
Environmental Biosafety Research, 5, 15–25.
Tatchell, G. M. (1983) Compensation in spring-sown oil-seed rape (Brassica napus L.) plants in response to injury to their flower buds and pods. Journal of Agricultural Science, 101, 565.
Wang, M. L., Zhao, Y., Chen, F. & Yin, X. C. (2004) Inheritance and potentials of a mutated dwarfing gene ndf1 in Brassica napus. Plant Breeding, 123, 449–453.
Warwick, S. I., Légère, A., Simard, M. J. & James, T. (2008) Do escaped transgenes persist in nature?
The case of an herbicide resistance transgene in a weedy Brassica rapa population. Molecular Ecology, 17, 1387–1395.
Weis, A., Simms, E. & Hochberg, M. (2000) Will plant vigor and tolerance be genetically correlated?
Effects of intrinsic growth rate and self-limitation on regrowth. Evolutionary Ecology, 14, 331–
352.
Zobel, M. (1992) Plant Species Coexistence: The Role of Historical, Evolutionary and Ecological Factors. Oikos, 65, 314–320.