There are no conflicts of interest associated with the authors and content of this paper.
There is no protected, financial, job-related or other private interest in a product, service and/or concern, what might influence the contents or positions that are described above in this manuscript.
ACKNOWLEDGEMENTS
The project promotion was made by the financial means of the Bundesanstalt für Landwirtschaft und Ernährung (BLE) and the medicinal concern Pfizer .
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Table 1. Study design with number of colostrum donor cows per experimental group, BNP (bovine neonatal pancytopenia) status of the colostrum fed to the calves, number of calves per experimental group and the reaction groups lethal clinical BNP, nonlethal clinical BNP,
Table 2. Analysis of variance for titres of BVDV(bovine viral diarrhea virus)-antibodies with the experimental group and the course of BNP as source for variation
Source of variation DF1 F-Value P-Value
Experimental group 4 8.49 0.000
Course of BNP 3 2.25 0.107
1Degrees of freedom.
Table 3. Analysis of variance for the blood cell counts PLT (platelets, G/l), WBC (leukocytes, G/l), and RBC (erythrocytes, T/l) using a mixed linear multivariate model considering the origin, the experimental group, the course of bovine neonatal pancytopenia (BNP) as fixed effects and the lifespan x course of BNP and the individual influence of the animal in the course of BNP (bovine neonatal pancytopenia) as random effect.
Cause of variation
DF1 F-value P-value
PLT WBC RBC PLT WBC RBC
Origin 8 1.41 1.57 3.07 0.191 0.135 0.002
Experimental
group 4 5.21 0.10 0.85 0.000 0.983 0.496
Course of
BNP 3 7.57 3.97 11.47 0.001 0.002 0.000
Lifespan x Course of BNP
71 9.02 4.65 2.69 <0.000 <0.000 <0.000
1Degrees of freedom.
0
Calves with lethal clinical BNP (n=19) Calves with nonlethal clinical BNP (n=2) Calves with subclinical BNP (n=14) BNP unaffected calves (n=8)
Control calves (n=9)
Platelets (G/l)
Age in days
Figure 1. Courses of platelet counts (median) in experimental calves by the course of BNP until the age of 27 days or until death.
0
Calves with lethal clinical BNP (n=19) Calves with nonlethal clinical BNP (n=2) Calves with subclinical BNP (n=14) BNP unaffected calves (n=8)
Control calves (n=9)
Leukocytes (G/l)
Age in days
Figure 2. Courses of leukocyte counts (median) in experimental calves by the course of BNP until the age of 27 days or until death.
SUPPLEMENTARY
Supplementary Figure 3. Bone marrow of a lethal clinical BNP (bovine neonatal pancytopenia) case, age at death: 7 days. The figure displays a hypocellular bone marrow. The intertrabecular medullar cavity contains reticular connective tissue with a loose stroma, which is partially filled with a moderately protein-rich fluid and adipose tissue. Only few hematopoietic precursor cells are evident. Hematoxylin-eosin stain, bar = 200 µm.
Supplementary Figure 4. Bone marrow of a lethal subclinical BNP (bovine neonatal pancytopenia) case, age at death: 9 days. The figure displays a hypocellular bone marrow. The intertrabecular medullar cavity contains reticular connective tissue with a loose stroma, which is partially filled with a moderately protein-rich fluid and adipose tissue. Only few hematopoietic precursor cells are evident. Hematoxylin-eosin stain, bar = 200 µm.