Chapter 6 Discussion
6.3 Conclusion
In conclusion, after i.s. TMEV infection, susceptible SJL and resistant B6 mice showed a clinical deterioration. Moreover, the observed clinical signs correlated with inflammatory, degenerative and demyelinating lesions within the SC, comparable to those seen in TMEV-IDD after i.c. infection of SJL mice, but with the advantage of a markedly shortened time span between infection and clinical onset as well as histopathological lesions. Moreover, the i.s. infection route enables the precise spatial and temporal investigation of virus spread and lesion sites. An addition advantage is that with this model also mice on a resistant B6 background can be used for studying inflammatory and demyelinating SC lesions. However, an establishment of virus persistence was only detected in i.s. infected SJL mice. Interestingly, after i.s. infection, inflammation, degeneration and demyelination were not restricted to the CNS but also occurred in PN of both, SJL and B6 mice. This peripheral neuropathy following i.s.
infection resembles important features of subforms of GBS, indicating its potential as a feasible model for human PNS diseases, making the i.s. TMEV-model an interesting alternative to classical i.c. infection. The obtained results once again clearly illustrate the profound impact of the injection site towards the clinical and pathohistological outcome following TMEV infection.
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ACKNOWLEDGEMENTS
37 Chapter 8 Acknowledgements
Finally, I would like to address my sincere gratitude to…
… Prof. Dr. Wolfgang Baumgärtner, PhD – my supervisor, for offering me the opportunity to join his excellent team and to work on this interesting project, his consistent academic guidance and criticism, his patience, encouragement and support.
… Prof. Dr. Andrea Tipold and Prof. Dr. Kirsten Haastert-Talini – my co-supervisors, for constructive comments and suggestions, as well as their collaboration and support throughout my study.
... PD Dr. Florian Hansmann, PhD for his brilliant ideas regarding scientific questions and solutions for technical problems, helping me in building self-confidence from failure and accomplishing this thesis and other scientific activities during the last years.
… all co-authors, especially Eva Leitzen, PhD for her outstanding contribution to the projects and great efforts in proofreading, fruitful discussions, and pleasant cooperation, as well as her help and support.
... the Max Planck Institute for Experimental Medicine in Göttingen, especially Prof.
Klaus-Armin Nave, PhD and Dr. Sandra Göbbels for providing the mice used for this study.
... the Medizinische Hochschule Hannover (MHH) in Hannover, especially Prof. Dr.
Kirsten Haastert-Talini, Nina Dietzmeyer, PhD and Zhong Huang for good collaboration and help.
... the coordination office of the Center for Systems Neuroscience (ZSN) and the international office of Stiftung Tierärztliche Hochschule Hannover, especially Maritta Ledwoch and Johanna Kroll for their kind help and great organization of activities of every kind.
… the China Scholarship Council (CSC) for financial support.
... the Niedersachsen research Network on Neuroinfectiology (N-RENNT) for the partial support of this project.
ACKNOWLEDGEMENTS
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… Kerstin Schöne, Caroline Schütz, Petra Grünig, Kerstin Rohn, Christiane amneck, Julia Baskas, Danuta Waschke and Claudia Herrmann for their excellent technical assistance.
... all my (former) colleagues from the Department of Pathology for their support and help during the four years, especially Angelika for tireless email-contact and help before I arrived Germany, Florian, Eva, Barbara and Ingo for their positive attitude, good sense of humor, kind manner and endless help, Eva, Rike, Elisa and Katarzyna for those interesting conversations and sharing one office with me and Aspen (sometimes), as well as Dandan, who was always by my side to support each other during this four years.
... my friends and my family, my parents and grandmother, as well as my grandfather, whom I intensely miss, for being the original source of my love and strength.