Bacterial and yeast strains and m ammalian cell lines…

7.1.1 Bacterial E. coli strains Table 7.1 dlacZ∆M15 ∆(lacZYA-argF)U169 endA1 recA1 hsdR17(rk

mk+

) deoRthi-1 supE44 λ^-gyrA96 relA1

Gibco-BRL Xl1Blu endA1 recA1 gyrA96 thi^- hsd R17(rk

mk+

∆ara-leu7697 ∆lacX74 ∆phoAPvuII phoR araD139 galE galK rspL F´[lac^-(lacI^q)pro] gor522::Tn10(Tc^r) trxB::kan (DE3)

Novagen

(Darmstadt, Germany)

BL21 F^- ompT hsdS (rB-mB-) gal dcm Novagen

BL21(DE3) F^- ompT hsdS (rB-mB-) gal dcm (DE3) Novagen

M15[pREP4] F^- Nal^S Str^S Rif^S Thi^- Lac^- Ara^- Gal^- Mtl^- recA⁺ Uvr⁺ Lon⁺ QIAGEN (Hilden) For a description of gene nomenclature see (Bachmann, 1987).

7.1.2 Yeast strains Table 7.2

Strain Genotype Source

cl3-ABYS-86MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R

D. H. Wolf, Univ. of Stuttgart, Germany MSUC-3D MATα ura3 trp1 leu2 his3 lys2 This Department

MSUC-IA MATa ura3 trp1 leu2 his3 ade8 This Department

sec18-1 MATα leu2 Dr. H. D. Schmitt,

this Department BHY11 MATa ura3-52 his3∆200 trp1∆901 ade2-101 suc2-∆9

leu2-3 112::pBHY11 (CPY-InvLEU2)

(Horazdovsky et al., 1994)

RH227-3A MATa ura3 leu2 his3 sec23-1 Prof. H. Riezman, Univ.

of Basel, Switzerland RPY18 MATa ura3 trp1 leu2 his3 ade8 sec24-11 (Peng et al., 1999) RPY61 MATa ura3 trp1 leu2 his3 ade8 sfb2::kanMX4 (Peng et al., 2000) RPY63 MATα ura3 trp1 leu2 his3 lys2 sfb2::kanMX4 sfb2::LEU (Peng et al., 2000) RPY72 MATα ura3 trp1 leu2 his3 ade8 sec24-11 sfb2::kanMX4 (Peng et al., 2000)

ADY1 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R SFB2-6His-2MYC-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000; Peng et al., 2000); This work ADY1/K^- MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R SFB2-6His-2MYC-loxP

This work

ADY2 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R SFB3-6His-2MYC-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000; Peng et al., 2000); This work ADY2/K^- MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R SFB3-6His-2MYC-loxP

This work

ADY3 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R SEC23-6His-2MYC-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000; Peng et al., 2000); This work

ADY4 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R SEC24-6His-2MYC-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000; Peng et al., 2000); This work ADY5 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1cps1-3 can^R

SFB2-6His-2MYC-loxP SFB3-6His-3HA-loxP-KanMX-loxP

(Peng et al., 2000);

This work ADY6 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1cps1-3 can^R

SFB2-6His-3HA-loxP-KanMX-loxP SFB3-6His-2MYC-loxP

(Peng et al., 2000);

This work ADY7 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R SEC24-6His-3HA-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000); This work ADY8 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1

prc1-1 cpsprc1-1-3 can^R SFB2-6His-3HA-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000); This work ADY9 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1

prc1-1 cpsprc1-1-3 can^R SFB3-6His-3HA-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000); This work.

ADY10 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R SEC24-6His-3VSV-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000); This work ADY11 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1

prc1-1 cpsprc1-1-3 can^R SFB2-6His-3VSV-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000); This work ADY12 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1

prc1-1 cpsprc1-1-3 can^R SFB3-6His-3VSV-loxP-KanMX-loxP

(De Antoni and Gallwitz, 2000); This work ADY13

MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cpsprc1-1-3 can^R SEC24-6His-3HA-loxP-KanMX-loxP SFB3-6His-2MYC-loxP

(De Antoni and Gallwitz, 2000); This work ADY14 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R GYP5-6His-2MYC-loxP-KanMX-loxP

This work ADY15 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R GYP5-6His-3HA-loxP-KanMX-loxP

This work ADY16 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R GYP5-6His-3VSV-loxP-KanMX-loxP

This work.

ADY20 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R gyp1::loxP-KanMX-loxP

This work ADY20/K^- MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp1::loxP

This work ADY21 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP-KanMX-loxP

This work ADY21/K^- MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP

This work ADY23 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp8::loxP-KanMX-loxP

This work

ADY24 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3 can^R gyp5::loxP gyp1::loxP-KanMX-loxP

This work ADY24/K^- MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP gyp1::loxP

This work ADY25 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP gyp8::loxP-KanMX-loxP

This work ADY25/K^- MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP gyp8::loxP

This work ADY26 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp1::loxP gyp8::loxP-KanMX-loxP

This work ADY27 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1 cps1-3

can^R gyp5::loxP gyp1::loxP gyp8::loxP-KanMX-loxP

This work ADY28 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP gyp8::loxP gyp1::loxP-KanMX-loxP

This work ADY29 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R ypt1^Q67L-LEU2

This work ADY30 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp1::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY31 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp5::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY32 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp7::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY33 MATα ura3-∆5 leu2-3 112 his3 pra1-1 prb1-1 prc1-1

cps1-3 can^R gyp8::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY40 MATα ura3 trp1 leu2 his3 lys2 gyp1::loxP-KanMX-loxP ^{This work} ADY40/K^- MATα ura3 trp1 leu2 his3 lys2 gyp1::loxP ^{This work} ADY41 MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP-KanMX-loxP ^{This work} ADY41/K^- MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP ^{This work} ADY42 MATα ura3 trp1 leu2 his3 lys2 gyp7::loxP-KanMX-loxP ^{This work} ADY43 MATα ura3 trp1 leu2 his3 lys2 gyp8::loxP-KanMX-loxP ^{This work} ADY43/K^- MATα ura3 trp1 leu2 his3 lys2 gyp8::loxP ^{This work} ADY44^- MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP

gyp1::loxP-KanMX-loxP

This work ADY44/K MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP gyp1::loxP ^{This work} ADY45 MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP

gyp8::loxP-KanMX-loxP.

This work ADY45/K MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP gyp8::loxP ^{This work} ADY46 MATα ura3 trp1 leu2 his3 lys2 gyp1::loxP

gyp8::loxP-KanMX-loxP.

This work ADY47 MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP gyp1::loxP

gyp8::loxP-KanMX-loxP.

This work ADY48 MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP gyp8::loxP

gyp1::loxP-KanMX-loxP.

This work ADY49 MATα ura3 trp1 leu2 his3 lys2 ypt1^Q67L-LEU2. ^{This work} ADY50 MATα ura3 trp1 leu2 his3 lys2 gyp1::loxP-KanMX-loxP

ypt1^Q67L-LEU2.

This work ADY51/K^- MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP ypt1^Q67L-LEU2 ^{This work} ADY52 MATα ura3 trp1 leu2 his3 lys2

gyp7::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work

ADY53 MATα ura3 trp1 leu2 his3 lys2 gyp8::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY54 MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP

gyp1::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY55 MATα ura3 trp1 leu2 his3 lys2 gyp5::loxP

gyp8::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work ADY56 MATα ura3 trp1 leu2 his3 lys2 gyp1::loxP

gyp8::loxP-KanMX-loxP ypt1^Q67L-LEU2

This work

For a description of gene nomenclature see (Rose et al., 1990; Sherman, 1991).

7.1.3 Mammalian tissue culture cell lines

The cell lines, the equipment, and materials for cell culture were gently provided by Prof. Mary Osborn (department of biochemistry and cell biology, MPI for Biophysical Chemistry). Cells were cultivated on Petri dishes in DME 10% FCS medium at 37 °C 95% humidity 7% CO2 (I was helped for cell culture by Heinz Juergen Dehne and Susanne Brandfass)

Table 7.3

Strain Origin Cell type Source

HeLa Human cervix adenocarcinoma epithelioid ATCC CV1 African green monkey kidney fibroblastic ATCC

Im Dokument Structural and functional analysis of yeast proteins involved in ER-to-Golgi transport: Sec24p family proteins and the GTPase activating protein Gyp5p (Seite 129-132)