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2.2 M OLECULAR DIFFERENTIATION BETWEEN ROOT - AND EARTHWORM - DERIVED BIOPORES IN SOIL BASED ON

2.2.7 Author contributions

YK and GW initiated the study. SH collected samples and organized laboratory analyses.

Laboratory work was conducted by G.W., S.H. and student helpers. GW conducted GC measurements and lipid data evaluation. SH performed statistical analyses. GW wrote the manuscript, while SH prepared figures, wrote the manuscript parts connected to linear discriminant analysis and significantly contributed to manuscript improvement. YK provided comments on the manuscript.

2.2.8 References

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2.2.9 Supplementary material

Supplementary material for

Molecular differentiation between root- and earthworm-derived biopores in soil based on free extractable fatty acids

#Guido L.B. Wiesenberg1*, #Silke Hafner2, Yakov Kuzyakov2,3

1 Department of Geography, University of Zurich. Winterthurerstrasse 190. CH-8057 Zurich, Switzerland.

2 Department of Soil Science of Temperate Ecosystems, University of Göttingen, 37077 Göttingen, Germany

3 Department of Agricultural Soil Science, University of Göttingen, 37077 Göttingen, Germany

# These authors contributed equally to this work

Table 2.2-4: One-way analysis of variance showing the effect of soil compartment on the discriminant scores for the linear discriminant function 1 (LD 1) and the linear discriminant function 2 (LD2). TukeyHSD post -hoc test was applied to reveal significant differences between each soil compartment. Degrees of freedom (df), F-values and significance level (p) are shown for soil compartment.

factor Discriminant scores LD 1 Discriminant scores LD 2

df F p df F p

compartment 2 139.30 *** 2 63.79 ***

Residuals 30 30

Tukey HSD diff p Diff p

BU - DS -2.67 *** -4.49 ***

BU - RS 4.26 *** -4.01 ***

RS - DS 6.93 *** 0.48

* p < 0.05

** p < 0.01

*** p < 0.001

Study 3

2.3 Spatial distribution and turnover of