3 Ergebnisse
4.10 Preise und Publikationen
Diese Dissertationsarbeit wurde unterstützt durch die Europäische Union durch das ‚Net-work of Excellence Biosim, Contract No. LSHB-CT-2004-005137’ sowie durch ein Stipen-dium der Medizinstiftung Marburg. Die Arbeit wurde im Dissertationswettbewerb im Rahmen der ‚Brain Awareness Week 2007’ durch Prof. Dr. Krieg, Prof. Dr. Oertel, Prof. Dr. Pagenste-cher und Prof. Dr. Sure zur besten neurowissenschaftlichen Dissertation der Universität Mar-burg ernannt.
Aus dieser Arbeit gehen einige Poster für Kongresse und Publikationen hervor:
Kongress-Poster
• Wegner S, Wollweber BT, Schneider H, Voigt H, Braun HA. (2006). Menthol effects in whole-cell voltage-clamp recordings of hypothalamic neurons. 2nd BioSim Confer-ence.
• Wollweber BT, Randolf A, Schäfer MKH, Wegner S, Postnova S, Voigt K, Braun HA. (2006). Modulation of Hypothalamic Temperature Sensitivity by Menthol. 2nd BioSim Conference.
• Wollweber BT, Wegner S, Randolf A, Schäfer MKH, Schneider H, Voigt K and Braun HA. (2007). Menthol effects on hypothalamic neurons. 7th Meeting of the German Neuroscience Society.
Publikationen
•
Wegner S, Schneider H, Braun HA, Voigt K, Wollweber BT. Menthol blocks potassium currents in an immortalized hypothalamic neuronal cell line. Manuskript.5 Literaturverzeichnis
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6 Anhänge
Verzeichnis der verwendeten Abkürzungen
4-AP 4-Aminopyridin
ADP Adenosindiphosphat
AS Aminosäure
ATP Adenosin-5´-triphosphat
BK big conductance K+-channels
CC Current clamp
CMR1 Cold and menthol receptor 1 (= TRPM8)
Cox2 Cyclooxygenase 2
CpG Cytosine paired Guanine
CRH Corticotropin-Releasing Hormone
Da Dalton
DAG Diacylglycerol
DMEM Dulbecco's Modified Eagle Medium DMSO Dimethylsulfoxid
DNA Desoxyribonuklein Acid
D-PBS Dulbecco's Phosphate Buffered Saline EDTA Ethylendiamintetraacetat
EGTA Ethylenglycol-bis(ß-aminoethylether) tetraacetic acid
ER endoplasmatisches Retikulum
FCS Fetal calf serum
FSH Follikelstimulierendes Hormon GABA Gamma-aminobutyric-acid
GDP Guanosin diphosphate
GnRH Gonadotropin releasing hormone
GTP Guanosin triphosphate
GPCR G-Protein gekoppelten Rezeptor
GT1 GnRH produzierende SV40 T-antigen transfizierte Zelllinie 1 HUVEC Human umbilical vein endothelal cell
I.E. Internationale Einheit
LH Luteinisierendes Hormon
IKCa Intermediate conductance Ca2+ dependent K+ channel (= SK4) IP3 Inositol-1,4,5-triphosphat
LPS Lipopolysaccarid
LTB4 Leukotrien B4
MW molecular weight
NADPH reduced nicotinamid adenine dinucleotid phosphate
OHC Outer hair cell
OVLT Organum vasculosum laminae terminalis
PAG Periaqueductal gray PGE2 / PGI2 Prostaglandin E2 / I2
PIP2 Phosphatidylinositol-4,5-bisphosphat
PKC Proteinkinase C
PLC Phospholipase C
mPOA Medial Preoptic Area
RES Reticuloendothelial system (= MPS) RMP Ruhemembranpotential RVD Regularory volume decrease
SK Small conductance potassium channel
TEA Tetraethylamonium
TMD Transmembran Domäne
TNF-α Tumor necrosis factor-α
TRPC Transient receptor potential classical type TRPM8 Transient receptor potential melanostatin 8 TRPV1 Transient receptor potential vanilloid type 1
TTX Tetrodotoxin
VC Voltage clamp
Nicht aufgeführt sind:
• Die Formelzeichen des Internationalen Einheitensystems (SI, Le Système
international d'unités) und deren abgeleitete Einheiten. Abkürzungen in Formeln sind dort angegeben.
• Die Abkürzungen für chemische Elemente. Sie entsprechen den Angaben des Periodensystems der Elemente nach der International Union of Pure and Applied Chemistry (IUPAC).
• Allgemein gebräuchliche Abkürzungen der deutschen Sprache nach dem Duden.
• Abkürzungen, die im Text erläutert sind.
Abbildungs- und Tabellenverzeichnis
Alle Abbildungen sind, sofern nicht anders in der Legende beschrieben, eigenständig von mir erstellt worden.
Abbildungen:
Abbildung 1.1 Regulation von Hunger und Sattheit im Hypothalamus ... 6
Abbildung 1.2 Regulation der GnRH-Ausschüttung... 10
Abbildung 1.3 Steuerung der Körpertemperatur, Einzelheiten siehe Text... 13
Abbildung 1.4 Elektrophysiologische Trennung der IK(A)- und IK(DR)-Ströme ... 15
Abbildung 1.5 α-Untereinheit eines spannungsabhänigigen K+-Kanal ... 16
Abbildung 1.6 α-Untereinheit des BKCa-Kanals... 18
Abbildung 1.7 Charakteristische Strom-Spannungskurve eines Kir-Kanals ... 19
Abbildung 1.8 Charakteristischer Na+-Einstrom durch die NaV zu Beginn eines Aktionspotentials... 22
Abbildung 2.1 Neubauer Zählkammer... 26
Abbildung 2.2 Übersicht über den Messstand... 27
Abbildung 2.3 Messkammer ... 28
Abbildung 2.4 Verstärker ... 29
Abbildung 2.5 Testpuls im Programm ‚Oszi’ ... 30
Abbildung 2.6 Messschuh ... 31
Abbildung 2.7 Puller ... 34
Abbildung 2.8 Microforge... 34
Abbildung 2.9 Coating-Stand... 35
Abbildung 2.10 Leckströme ... 39
Abbildung 2.11 Patch-Clamp Konfigurationen ... 40
Abbildung 2.12 Strom-Spannungs-Diagramm mit Umkehrpunkt bei -60 mV... 43
Abbildung 2.13 Doppelkap Protokoll zur Bestimmung der Membrankapazitäten ... 45
Abbildung 2.14 Bestimmung des Membranpotentials anhand eines Strom-Spannungs-Diagramms . 45 Abbildung 2.15 Spannungsklemme... 46
Abbildung 2.16 APs in der Stromklemme... 47
Abbildung 3.1 PCR ... 53
Abbildung 3.2 Ca2+-Imaging der GT1-Zellen... 54
Abbildung 3.3 Hemmung der Auswärtsströme durch Menthol, 4-AP und TEA... 56
Abbildung 3.4 Strom-Spannungs-Diagramme unter Hoch-K+-Badlösung ... 58
Abbildung 3.5 Charakterisierung der K+-Ströme ... 60
Abbildung 3.6 Dosis-Wirkungskurve der Mentholinduzierten Hemmung des Auswärtsstroms ... 61
Abbildung 3.7 Strom-Spannungsdiagramm bei Na+-Isolation... 63
Abbildung 3.8 Messungen in Ca2+ freier Badlösung ... 65
Abbildung 3.9 Bestimmung der Leitfähigkeit eines Einzelkanals ... 66
Abbildung 3.10 Fotos der GT1-7 Zellen in verschiedenen Zuständen... 67
Abbildung 4.1 Kinetiken und Modifizierung der KV1-Kanäle... 71
Abbildung 4.3 Applikation von 3 mM Menthol ... 73
Tabellen:
Tabelle 1.1 Übersicht über die Blocker der verschiedenen K+-Kanäle ... 21 Tabelle 2.1 Intra- und extrazelluläre Konzentrationen sowie die Nernstpotentiale der wichtigsten Ionen
... 42 Tabelle 4.1 Pipettenlösungen verschiedener Autoren im Vergleich ... 69 Tabelle 4.2 Badlösungen verschiedener Autoren im Vergleich ... 69
Liste der akademischen Lehrer
Meine akademischen Lehrer…
…in Marburg
Aumüller, Basler, Baum, Bien, Bischof, Boudriot, Braun, Breckle, Cruse, Eilers, Elsässer, Engelhaaf, Garten, Geger, Gerdes, Grundmann, Gudermann, Hamer, Hanley, Hasilik, Heeg, Heimann, Hermann-Lingen, Hertl, Jungclas, Kircher, Klenk, Kogel, Konrad, Krieg, Kroll, Kunz, Lang, Langer, Leube, Lill, Löffler, Lohoff, Maier, Mandrek, Martin, Mederos-Schnitzer, Moll, Müller, Mueller, Musset, Neuert, Oeffner, Oertel, Opitz, Plant, Rager, Renz, Riera, Röhm, Röper, Rosenow, Schäfer, Schimke, Schlotzhauer, Schmidt, Schneider, Schofer, Sommer, Schrader, Schu, Schulz, Stiller, Suske, Vogelmeier, Vogt, Voigt, Weihe, Weiler, Werner, Westermann, Wulf, Werner.
… in Bielefeld
Bartolomaeus, Dietz, Feldmann, Jockusch, Reißland, Ruppel, Tschirnhaus, Weber
… in Lippstadt Marke, Matz, Kogel
… in Palma de Mallorca/Spanien
Gessner, Hörstmann, Linnenbecker, Terasa
… in Alicante/Spanien
Candela Gomis, Compañ Rosique, Diez Miralles ... in Schwalmstadt
Frick, Hesselbarth, Puplat, Schulze, Weber