- 69 - 8. Appendix
- 70 -
PCR Polymerase Chain Reaction
rpm Revolutions per minute
SDF-1α Stromal cell-derived factor-1α
SDS Sodium-Dodecyl-Sulphate
SIV Simian immunodeficiency virus
STAT 3 Signal transducer and activator of transcription 3 STR Short tandem repeat analysis
TEER Transepithelial electrical resistance TEMED N, N, N', N'-tetramethyl ethylenediamine
WT Wild type
- 71 - 8.2 Table index
Table 1 11
Table 2 15
Table 3 19
Table 4 21
Table 5 21
Table 6 21
- 72 - 8.3 Figure index
Figure 1 2
Figure 2 3
Figure 3 4
Figure 4 16
Figure 5 25
Figure 6 29
Figure 7 30
Figure 8 31
Figure 9 32
Figure 10 33
Figure 11 34
Figure 12 35
Figure 13 36
Figure 14 38
Figure 15 39
Figure 16 41
Figure 17A 42
Figure 17B 43
Figure 18 45
Figure 19 46
Figure 20 47
Figure 21 48
- 73 - 8.4 Presentations
“Differential expression of VEGF-A isoforms in angiomas and squamous cell carcinoma cell lines of the head and neck area.”
2nd International Symposium on Hemangiomas and Vascular Malformations of the Head and Neck, Little Rock, USA, 2009.
“SIV Nef as a new therapeutic approach in head and neck cancer.”
Workshop zur klinischen und experimentellen Forschung in der Kopf-Hals-Onkologie, Leipzig, Germany, 2011.
“Implications of SIV-Nef in antiangiogenesis.”
3rd International Symposium on Hemangiomas and Vascular Malformations of the Head and Neck, Marburg, Germany, 2011.
- 74 - 8.5 Curriculum vitae and publications
Name : Gender :
Chengzhong Cai male
Date of birth : Marital status:
8th October 1974 Married Tel :
(+49)-017634147136 E-mail:
chengzhong.cai@gmail.com
Education experience
Medical College of Philipps-University, Marburg, Germany October 2008 – present
Doctoral thesis
Medical College of TongJi University, Shanghai, China.
October 2004 – December 2006 Master degree of Medicine –Surgery
Changzheng Hospital, Shanghai, China.
September 2004 – April 2005
Visiting scholar, Central Lab of Molecular Biology. Field of research: “The expression of E-cadherin in gastric cancer.”
Medical College of Tiedao University, Shanghai, China.
September 1993 – July 1998 Bachelor degree of Medicine
- 75 - Working experience
University Hospital Giessen and Marburg, Marburg, Germany.
November 2008 – present Doctoral fellow of Prof. Mandic
Shanghai Tenth People’s Hospital, China.
2003 - November 2008 Attending Doctor.
Shanghai Tenth People’s Hospital, China.
August 1998 – 2003 Resident Doctor.
Qualifications
Doctor’s Qualification Certification of The People’s Republic of China.
December 1999
Doctor’s Practice Certification of The People’s Republic of China.
December 2001
Qualification Certificate of Speciality and Technology of The People’s Republic of China. ( General Surgery )
September 2003
Teacher’s Qualification Certification (College level) in Clinical Medicine of The People’s Republic of China.
January 2004
- 76 - Membership
Chinese Medical Association
Angioma group, University Hospital Giessen and Marburg, Germany
German Society of Oto-Rhino-Laryngology, Head and Neck Surgery
Publications
1. Cai C, Böttcher MC, Werner JA, Mandic R. Differential expression of VEGF121, VEGF165 and VEGF189 in angiomas and squamous cell carcinoma cell lines of the head and neck. Anticancer Res. 2010;
30(3):805-10.
2. Martina C. Boettcher, Behfar Eivazi, Marion Roessler, Michael Bette, Cai C, Susanne Wiegand, Christian Güldner, Jochen A. Werner, Robert Mandic. Involvement of LYVE-1-positive endothelial cells in the formation of non-lymphatic vascular malformations. Histopathology. 2010; 57(5):
763–770.
3. Teng HF, Wang S, Ge HY, Zhu WH, Cai C, Jiang X. Thyroidectomy through suprasternal approach under endoscope. Joural of TongJi university(medical science). 2008; 6.
4. Ge HY, Hao ZH, Jiang SL, Cai C, Jiang X. The clinical experience of lobectomy of thyroid with small cervical incision. Journal of Endocrine Surgery. 2008; 2(1).
5. Cai C, Xu HW, Tan LY. To establish a method of semi-quantitative RT-PCR with two-step-circle in separated tube to detect E-cadherin levels in gastric cancer cells. China Clinic Cancer. 2006; 33(8): 4-6.
- 77 -
6. Cai C, Xu HW, Tong XC. High Expression of E-cadherin in serum and tissues of colorectal Cancer. World Chinese Journal of Digestology. 2004;
12(6): 1481-1483.
7. Xu HW, Wang L, Tong XC, Cai C. Correlation between transforming growth factor ß1 and its receptor and stomach carcinoma aggressiveness.
Acad J Sec Mil Med Univ. 2004; 25(10): 1149-1150.
8. Xu HW, Cai C, Tan LY. Relationshinp of serum and tissue E-cadherin levels and radical operation on stomach carcinomas. Chin J EXP Surg.
2003; 20(8): 751-752.
9. Xu HW, Cai C, Tong XC. The influence of aggressiveness and surgical operation of colorectal carcinomas on serum E-cadherin level. Cancer Research on Prevention and Treatment. 2003; 30(3): 190-191.
10. Xu HW, Cai C, Tan LY. The influence of aggressiveness on serum E-cadherin Level in patients suffering from stomach carcinoma. China Clinic Cancer. 2003; 30(8): 533-535.
Language Skills:
English
German
Chinese
- 78 - 8.6 List of academic teachers:
Main academic teachers in Tongji University, Shanghai, China.
Ms. / Mr :
Cai, Chen, Fan, Ge, Gu, Guo, Li, Liu, Luo, Wei, Xiong, Zhang
Main academic teachers in Philipps-University, Marburg, Germany.
Ms. / Mr :
Bette, Eivazi, Mandic, Ocker, Sesterhenn, Teymoortash, Weiß, Werner
- 79 - 8.7 Acknowledgements
I thank Prof. Dr. med. Jochen A. Werner for his kind financial support during these three years. Here, I want to show my great appreciation to my supervisor, Prof. Dr. med. Robert Mandic, for his patient education, kindly help and also for his strong pressure to keep me forward. I also thank my colleagues Ms. Roswitha Peldszus, Ms. Maria Sadowski, Ms. Annette Rossmann, Ms. Marie Meinold, Ms. Thuridur Jonsdottir, Ms. Galina Makarova, Ms. Branka Malidzan, Ms. Ariane Rath, Ms. Fiona Rodepeter, Mr. Christian Wiemers (Dept. of Otolaryngology, Head & Neck Surgery, UKGM GmbH) and our co-operators Priv. Doz. Dr. Michael Bette (Department of Anatomy and Cell Biology, Philipps-University Marburg, Germany), Mr. Thorsten Volkmann (Dept.
of Hematology, Oncology and Immunology, UKGM GmbH), Prof. Dr. med.
Matthias Ocker, Dr. med. Karl Quint and Dr. Pietro Di Fazio (Institute for Surgical Research, UKGM GmbH) for their excellent technical assistance. I also want to show my thanks to Prof. Dr. med. Afshin Teymoortash, Prof. Dr.
med. Andreas Sesterhenn, Dr. med. Rainer Weiß, Dr. med. Behfar Eivazi and other doctors (Dept. of Otolaryngology, Head & Neck Surgery, UKGM GmbH) for their supports and kindly help during my practice in clinic.
At last but not least, I want to thank my family, especially my wife Hui Chen, I could not have succeeded without their support. I am dedicating my doctoral thesis as a gift to my new born daughter Ouchun Cai. I love you and miss you so much. I hope you will come back to Marburg when you grow up.