Abbreviations

°C degrees celsius

LC-MS/MS liquid chromatography-tandem mass spectrometry

3C chromosome conformation capture

4-OHT 4-hydroxytamoxifen 53BP1 p53-binding protein 1

aa amino acids

Ac acetylation

Acetyl-CoA acetyl coenzyme A

Acf1 ATP-dependent chromatin assembly factor 1

ADP adenosine diphosphate

AEL after egg laying

AID auxin-inducible-degron

Alc1 amplified in liver cancer protein 1 ATF2 activating transcription factor 2 ATM ataxia telangiectasia mutated protein

atms antimeros

ATP adenosine triphosphate

ATR ataxia telangiectasia and Rad3-related protein Atrip ATR-interacting protein

Atu another transcription unit

Baf barrier-to-autointegration factor

Baz1A bromodomain adjacent to zinc finger domain 1a Beaf boundary element associated factor

beb both ends bound

Blm bloom syndrome protein

bp base pairs

Brca1/2 breast cancer type 1/2

Brct Brca1 C-terminal

Bruce BIR repeat containing ubiquitin-conjugating enzyme

BSA bovine serum albumin

C control

CaCl2 calcium chloride

Cdc cell division cycle protein

Chd chromodomain-helicase-DNA-binding protein

ChIP chromatin immunoprecipitation

CHRAC chromatin accessibility complex

Chz1 chaperone for Htz1

contr control

Ctcf corrected total cell fluorescence

CtIP C-terminal-binding protein interacting protein Ctr9 RNA polymerase-associated protein CTR9 homolog

Cul4 cullin4

CuS04 copper sulphate

Da Dalton

DAPI 4'.6-diamidino-2-phenylindole dATP deoxyadenosine triphosphate dCTP deoxycytidine triphosphate

Ddb DNA damage-binding protein

ddH2O double distilled H2O

dGTP deoxyguanosine triphosphate

DMSO dimethylsulfoxide

DNA deoxyribonucleic acid

DNA-PK DNA-dependent protein kinase

DNA-PKcs DNA-dependent protein kinase, catalytic subunit Dna2 DNA replication ATP-dependent helicase/nuclease 2 dNTP deoxynucleotide triphosphate

Dom domino

Dot1 disruptor of telomeric silencing DREX Drosophila embryonic extract

DSB double strand break

dsDNA double-stranded DNA

DTT 1.4-dithiothreitol

dTTP deoxythymidine triphosphate dUTP deoxyuridine triphosphate E. coli Escherichia coli

ecd ecdysoneless

EDTA ethylenediaminetetraacetic acid

eEF1 eukaryotic translation elongation factor 1 alpha 1

Ef1 elongation factor 1

EGTA ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid

Elp3 elongator complex protein 3

ER estrogen receptor

et al. et alii

Etl1 enhancer trap locus 1

Exo1 exonuclease 1

FAIRE formaldehyde-assisted Isolation of regulatory elements

FBS fetal bovine serum

Fcp1 TFIIF-interacting CTD phosphatase 1

Fen1 flap endonuclease 1

FlyFos fly fosmid

Fun30 function unknown now 30

FV forward

g gram

g gravitational acceleration

G1/G0-phase gap1/gap0-phase

Gcn5 general control nonderepressible 5

GO gene ontology

GST glutathione S-transferase

H2A.V histone H2A variant

HA hemagglutinin

HAT histone acetyltransferase

Hepes N-(2-hydroxyethyl)piperazine-H´-(2-ethanesulfonic acid) Hpa2 histone and other protein acetyltransferase 2

HR homologous recombination

Hsp70 heat shock protein 70 kDa

Hyx hyrax

I inhibitor

Ida imaginal discs arrested

IF immunofluorescence

IgG immunoglobulin G

Ino80 inositol-requiring protein 80

IP immunoprecipitation

IPTG isopropyl-D-thiogalactopyranoside)

IR infrared

Irbp inverted repeat binding protein

Iswi imitation switch

k kilo

kb kilobase

KCl potassium chloride

KD knock down

kis kismet

Klp10A kinesin-like protein 10A

KO knock out

L liter

LB lysogeny broth

Lig3 ligase 3

Lig4 ligase 4

M marker

m milli

M molar

Mdc1 mediator of DNA damage checkpoint protein 1

mdlc midlife crisis

me methyl

Mei-41 meiotic 41

MgCl2 magnesium chloride MNase micrococcal nuclease

mod modulo

Mof males absent on the first

Mre11 meiotic recombination 11

Mrg15 mortality factor 4 related gene 15

MRN Mre11-Rad50-Nbs1

msn mishappen

n nano

NaCl sodium chloride

NAD nicotinamide adenine dinucleotide

NaMBS sodium m-maleimidobenzoyl-N-hydroxysuccinimide ester Nap1 nucleosome assembly protein 1

Nbs1 nibrin 1

NDS normal donkey serum

NFR nucleosome-free region

NGS normal goat serum

NHEJ non-homologous end joining

NuA4 nucleosome acetyltransferase of histone H4 NuRD nucleosome remodeling deacetylase

oeb one end bound

P phosphate

p300 HAT histone acetyltransferase p300 p400 E1A-associated protein p400

PAR Poly-(ADP-ribose)

ParB chromosome-partitioning protein B

Parp1 Poly-(ADP-ribose) polymerase 1

PBS phosphate buffered saline

Pcaf P300/CBP-associated factor

PcG Polycomb Group

PCR polymerase chain reaction

PEG polyethylene glycol

PGC primordial germ cell

pH potential of hydrogen

PhD doctor of philosophy

PHD plant homeodomain

Phf7 PHD Finger Protein 7

PMSF Phenylmethanesulfonyl fluoride

Pont pontin

PP2A protein phosphatase 2

PP4R2r protein phosphatase 4 regulatory subunit 2-related protein PRC1 Polycomb repressor complex 1

PRC2 Polycomb repressor complex 2 Prp8 pre-mRNA processing factor 8 PTM post-translational modification

puf puffyeye

Rad50 radiation sensitive 50/51

RanBP Ran-binding protein

Ring really interesting new gene

RNA ribonucleic acid

RNA pol II RNA polymerase II

RNAi RNA interference

RNAse ribonuclease

Rnf8 ring finger protein 8

Roc1 homeobox-leucine zipper protein

row relative of woc

RPA replication protein A

rpm revolutions per minute

RpS29 Ribosomal protein S29

rRNA ribosomal RNA

RSF remodeling and spacing factor Rsf1 repressor splicing factor 1

RV reverse

S-phase synthesis phase

S2 Drosophila Schneider cells

Sae2 sporulation in the absence of spo eleven 2

Sas2 something about silencing 2

Scu scully

SD standard deviation

SDS sodium dodecyl sulphate

SDS-PAGE SDS-polyacrylamide gel electrophoresis

Seq sequencing

Set8 SET domain-containing protein 8

SETMAR SET Domain And Mariner Transposase Fusion Protein

Sf21 Spodoptera frugiperda 21

sle slender lobes

Smarca5 Swi/Snf -related matrix-associated actin-dependent regulator of chromatin subfamily A member 5

Smarcad1 Swi/Snf -related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

SN supernatant

Snf2h sucrose nonfermenting-2 homolog Snf2l sucrose nonfermenting-2 like

spn-A spindle A

SRCAP Snf2-related CBP activator protein

ssDNA single-stranded DNA

ssh slingshot

Su(Hw) suppressor of hairy wing Suv39h1 Suv4-20 suppressor of variegation 4-20 homolog

SWATH sequential window acquisition of all theoretical fragment ion spectra Swi/Snf switch/sucrose non-fermentable

Swr1/2 Swi2/Snf2-related 1/2

TAD topologically associated domains

TBS Tris buffered saline

TE Tris-EDTA

Tefu telomere fusion

TEMED N,N,N´,N´-tetramethylethylenediamine Tip60 tat interactive protein 60kDa

tou toutatis

TRAX transcription extract TSS transcription start site

U unit

UAS upstream activating sequence

Ub ubiquitin

UV ultraviolet

v/v volume per volume

Vta1 vacuolar protein sorting-associated protein 1

w/v weight per volume

WB Western blot

Woc without children

wt wild type

Xpc Xeroderma pigmentosum, complementation group C

Xlf Xrcc4-like factor

Xrcc X-Ray Repair Cross Complementing

YL1 vacuolar protein sorting-associated protein 72 homolog 1 γH2A.V phosphorylated H2A.V

µ micro

Im Dokument In vitro chromatin reconstitution as a tool to study H2A.V and the DNA damage response (Seite 151-156)