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Contribution of Chlorophyll Fluorescence to the Reflectance of Leaves in Stressed Plants as Determined with the VIRAF-Spectrometer

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Contribution of Chlorophyll Fluorescence to the Reflectance of Leaves in Stressed Plants as Determined with the VIRAF-Spectrometer

Claus Buschmann* and Hartmut K. Lichtenthaler

Botanisches Institut II, Universität Karlsruhe, D-76128 Karlsruhe, Germany.

Fax: +49 721 608 4874. E-mail: Claus.Buschmann@bio-geo.uni-karlsruhe.de

* Author for correspondence and reprint requests

Z. Naturforsch.54c, 849Ð855 (1999), received May 20, 1999

Chlorophyll Fluorescence, Red Edge of Reflectance, Remote Sensing, Spectroscopic Techniques, Stress Detection

Reflectance spectra allow the early detection of stressors causing differences in pigment content as well as changes of leaf tissue structure and photosynthetic activity. The reflectance decreased with increasing Chl content in greening bean leaves. In stressed leaves, in turn, the reflectance increased with decreasing Chl content. This also caused a shift of the red reflection rise (“red edge”) to shorter wavelengths (“blue shift”) associated with a blue shift of the inflection point of the red edge. The contribution of the red and far-red Chl fluores- cence to the reflectance signal at the red edge of the spectrum and the shift of the wavelength position of the inflection point are demonstrated and discussed.

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