Wissenschaftliche Beiträge TH Wildau 2016

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Wissenschaftliche Beiträge TH Wildau

2016

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IMPRESSUM

HERAUSGEBER

Der Präsident der Technischen Hochschule Wildau Prof. Dr. László Ungvári

Hochschulring 1 15745 Wildau www.th-wildau.de

REDAKTIONSKOLLEGIUM DER TH WILDAU Prof. Dr. sc. hum. Marcus Frohme

Prof. Dr. rer. pol. Sandra Haas Prof. Dr.-Ing. Michael Hendrix Prof. Dr. rer. nat. Michael Herzog Prof. Dr.-Ing. Jörg Reiff-Stephan Prof. Dr. phil. Olga Rösch

REDAKTION

Markus Vossel, René Grube Telefon + 49 (0) 3375 / 508 - 673 markus.vossel@th-wildau.de

GESAMTHERSTELLUNG

STÜRMER & DRÄNGER – Visuelle Kommunikation Richard-Sorge-Straße 24

10249 Berlin

Telefon (030) 48 62 47 64 kontakt@stuermer-draenger.de www.stuermer-draenger.de Titelfoto: Shutterstock

ISSN 0949-8214 20. Jahrgang

Nachdruck einzelner Artikel und Beiträge bei Quellenangabe frei.

*KGTȨPFGP5KGFKG#TVKMGNFGU

20. Jahrgangs zum kostenlosen Download.

www.th-wildau.de/forschung/wissbeitraege.html

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WISSENSCHAFTLICHE BEITRÄGE

2016

1

THWildau

Wissenschaftliche Beiträge 2016

ANGEWANDTE BIOWISSENSCHAFTENINFORMATIK / TELEMATIKOPTISCHE TECHNOLOGIEN / PHOTONIKPRODUKTION UND MATERIALVERKEHR UND LOGISTIKMANAGEMENT UND RECHT

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INHALTSVERZEICHNIS

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$KQGNGMVTQMCVCN[UGFGU'P\[OUPyrrolochinolinchinon-abhängige Glukosedehydrogenase

Johannes Gladisch, David Sarauli, Daniel Schäfer, Birgit Dietzel, Burkhard Schulz, Fred Lisdat

23 '%5-QORNGZsGKPPGWGT$KQOCTMGTDGK9CEJUVWOUJQTOQPUVÒTWPIGP!

Wilhelm Gossing, Lars Radke, Marcus Frohme, Henrik Biering

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Falko Schmalenberg, Ralf Vandenhouten

37 #/QFWNCT/QDKNG&GXKEGHQT4GCN6KOG&5VTGCOKPI

Ralf Vandenhouten, Richard Fiebelkorn, Andrea Funke

126+5%*'6'%*01.1)+'02*1610+- 45 #FXCPEGF0CPQRJQVQPKEU5KNKEQP1TICPKE*[DTKF6GEJPQNQI[

Patrick Steglich, Claus Villringer, Silvio Pulwer, Joachim Bauer, Friedhelm Heinrich, Mauro Casalboni, Sigurd Schrader

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Jörn Bräske, Steffen Klohs, Thomas Mirre

59 8GTINGKEJXQP+ORNGOGPVKGTWPIUOÒINKEJMGKVGPKPFWUVTKGNNGT 'PGTIKGOQPKVQTKPIU[UVGOGHØT-/7

René von Lipinski, Dirk Buße, Jörg Reiff-Stephan

67 1RVKOKGTWPIXQP'RQZKFJCT\U[UVGOGPHØT2'4QJTKPPGP DGUEJKEJVWPIGPCNU5CWGTUVQHHURGTTUEJKEJV

Eckhart Kornejew, Harald Goering, Michael Herzog

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Valery M. Samuylov, Oksana D. Pokrovskaya, Lyudmila V. Gashkova, Herbert Sonntag

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Matthias Forster, Victoria Scholl

89 9JCV&TKXGUVJG5WEEGUUQH2QR7R5VQTGU!

Sandra Haas, Lisa Schmidt

97 &KG#DQ%QOOGTEG.CPFUEJCHVKP&GWVUEJNCPF 'KPGCPIGDQVUDG\QIGPG7PVGTUWEJWPI

Sandra Haas, Torben Schmolke

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Frank Hartmann, Dana Mietzner

3

THWildau

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VORWORT

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Zum Schluss wünsche ich Ihnen eine anregende Lektüre der Wis- senschaftlichen Beiträge 2016. Die Artikel sind auch als PDF über das Internet abrufbar, besuchen Sie dazu die Internetseite

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Prof. Dr. László Ungvári 2TÀUKFGPVFGT6*9KNFCW

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THWildau

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)GPQOYGKVG+FGPVKȨ\KGTWPIXQPEJTQOQUQOCNGP

$TWEJRWPMVGPDGK6WOQTGPWPVGTUEJKGFNKEJGT)GYGDG

Birgit Bortz, Stefanie Marczok, Heike Pospisil*

Zusammenfassung

#WHITWPFFGTLÀJTNKEJCPUVGKIGPFGP#P\CJNCP-TGDUPGW- GTMTCPMWPIGP WPF FGT VÒFNKEJGP 8GTNÀWHG XQP OCNKIPGP 6WOQTGPIGYKPPVFKGXQNNUVÀPFKIG#WHMNÀTWPIFGT6WOQT- IGPGUGWPFRTQITGUUKQPKOOGTOGJTCP$GFGWVWPI(ØT FKGUGMÒPPGP7PVGTUWEJWPIGP\W$TWEJRWPMVGPFKGGKPG -QRKGP\CJNXCTKCVKQP %08 KP -TGDUIGPQOGP DGYKTMGP IGPWV\VYGTFGP'UYWTFGGKPG2KRGNKPGGPVYKEMGNVFKGKP FGT.CIGKUV%08U$TWEJRWPMVTGIKQPGP$24UWPF)GPG IGPQOYGKVOKV*KNHGXQP502#TTC[&CVGP\WFGVGMVKGTGP

&C\WYWTFGP6WOQTRTQDGPCWU|6WOQTGPVKVÀVGP WPVGTUWEJV WPF OKV 2TQDGP CWU IGUWPFGO )GYGDG XGTINKEJGP +P FGP 6WOQTRTQDGP YWTFGP XKGTHCEJ OGJT

$24U FGVGMVKGTV YQDGK WPVGT | FGT )GPG KP FGP 0QT- OCNRTQDGPDGVTQHHGPUKPF9KTKFGPVKȨ\KGTVGPJQEJURG-

\KȨUEJG$24U&KGCOJÀWȨIUVGPXQTMQOOGPFG8CTKCVKQP WOUEJNKG»VFCU)GPKIAA0513YGNEJGUKP8GTDKPFWPIOKV FGT#RQRVQUGUVGJV#PJCPFFGTJKGTGPVYKEMGNVGP2KRGNKPG MÒPPGPGTUVG'KPDNKEMGKP%08WPF$TWEJRWPMV/WUVGTKP 6WOQTIGPQOGPIGYQPPGPYGTFGPFKG\WGKPGOXGTDGU- UGTVGP8GTUVÀPFPKUFGT6WOQTIGPGUGWPFUQOKV\WGKPGT XGTDGUUGTVGP &KCIPQUVKM WPF 6JGTCRKG XQP -TGDUGTMTCP- MWPIGPHØJTGPMÒPPGP

Abstract

6JGPWODGTQHPGYECPEGTECUGUCPFVJGFGCFN[EQWTUGU QHOCNKIPCPVVWOQTUCTGUVKNNTKUKPI#PGPVKTGENCTKȨECVKQP QH VWOQTKIGPGUKU CPF RTQITGUUKQP KU TGSWKTGF HQT DGVVGT FKCIPQUVKEU CPF VJGTCRKGU *GTG YG RTGUGPV CP CPCN[UKU QHIGPQOKEDTGCMRQKPVRCVVGTPUYJKEJOKIJVECWUGEQR[

PWODGT XCTKCVKQPU %08U KP ECPEGT IGPQOGU 6JWU C RKRGNKPG YCU FGXGNQRGF YJKEJ WUGU 502 CTTC[ FCVC HQT VJGIGPQOGYKFGFGVGEVKQPQH%08UDTGCMRQKPVTGIKQPU

$24UCPFIGPGUVWOQTUCORNGUHTQOGKIJVVWOQT GPVKVKGU YGTG CPCN[\GF CPF EQORCTGF YKVJ UCORNGU HTQOJGCNVJ[VKUUWG(QWTVKOGUOQTG$24UYGTGFGVGEVGF KPVJGVWOQTUCORNGUCPFQPN[NGUUVJCPQHVJGCHHGEV- GFIGPGUYGTGHQWPFVQDGKPXQNXGFKPJGCNVJ[VKUUWGU 9G KFGPVKȨGF JKIJN[ URGEKȨE $24U 6JG OQUV EQO- OQPN[ QEEWTTKPI XCTKCVKQP HQWPF KP QWT UVWF[ GPENQUGU the KIAA0513 IGPG YJKEJ KU CUUQEKCVGF YKVJ CRQRVQUKU

$CUGFQPVJGUGTGUWNVUPGYKPUKIJVUKPVWOQTKIGPGUKUCTG GZRGEVGF

'KPNGKVWPI

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MWPIGP \WPKOOV ,GFQEJ YGTFGP

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+PGKPGTWPUGTGTHTØJGTGP5VWFKGPMQPP- VGPYKTHGUVUVGNNGPFCUUOKVUVGKIGPFGO 6WOQTUVCFKWOXGTOGJTV4GIKQPGPOKV XGTÀPFGTVGP-QRKGP\CJNGPFGVGMVKGTDCT UKPF #WHITWPF FGUUGP NCI FKG 8GTOW- VWPI PCJG FCUU FKG 'PVUVGJWPI XQP

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%08UCWHVTGVGP&KGUG$GTGKEJGYGTFGP KPFGT.KVGTCVWTCNUfragile sitesDG\GKEJ- PGV+PFKGUGT#TDGKVYWTFGWPVGTUWEJV QD FKGUG $GTGKEJG HØT XGTUEJKGFGPG 6WOQTCTVGP URG\KȨUEJ UKPF WO GKPGP DGUUGTGP'KPDNKEMKPFKG6WOQTRTQITGU- UKQPWPFCWHFKGIGPQOKUEJGP#WUYKT- MWPIGP \W GTJCNVGP 5VCPFHW» GV CN #NNGTFKPIU DCUKGTVG FKGUG #TDGKV CWHYGPKIGP2TQDGP

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7O FKG 8GTÀPFGTWPI FGT -QRKGP\CJN WPF FKG FC\WIGJÒTKIGP $TWEJRWPM- VG KP 6WOQTIGPQOGP FGVGMVKGTGP \W MÒPPGP YGTFGP XGTUEJKGFGPG 6GEJ- PQNQIKGP IGPWV\V &KG DGUVG #WȩÒ- UWPI FGT 'TIGDPKUUG NKGHGTP FGT\GKV

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UKG GKP IWVGU 8GTJÀNVPKU XQP FGT RJ[- UKMCNKUEJGP #DFGEMWPI WPF 5EJPGN- NKIMGKVFGT#PCN[UGDKGVGP<JCQGVCN

<WFGOKUVFGT8QTVGKNFGT502

#TTC[#PCN[UGP FCUU GKP ITQ»GT &C- VGPDGUVCPF HTGK \WIÀPINKEJGT &CVGP- UÀV\GXQTJCPFGPKUV

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Maligne Tumordaten:&KGXGTYGPFG- VGP 6WOQTFCVGP HØT FKG IGPQOYGKVG +FGPVKȨ\KGTWPI XQP $TWEJRWPMVTGIKQ- PGP$24UWPF5GIOGPVGPXGTÀPFGT- VGT -QRKGP\CJN YWTFGP FGT ÒHHGPVNKEJ

\WIÀPINKEJGP )'1Gene Expression Omnibus &CVGPDCPM XQO 0%$+ Na- tional Center for Biotechnology Infor- mationGPVPQOOGP0CVKQPCN%GPVGT HQT $KQVGEJPQNQI[ +PHQTOCVKQP

#WU FKGUGT &CVGPDCPM YWTFGP PWT

&CVGP FKG OKVVGNUGenome-Wide Hu- man SNP Array 6.0CPCN[UKGTVYWTFGP XGTYGPFGV +PUIGUCOV GTHQNIVG FKG

#PCN[UGXQPOCNKIPGPRTKOÀTGP 6WOQTRTQDGP CWU XGTUEJKGFGPGP 6WOQTGPVKVÀVGP )GJKTPVWOQTG s 2TQDGP$TWUVVWOQTGs2TQ- DGP -QNQTGMVCNVWOQTG s 2TQDGP .WPIGPVWOQTGs2TQDGP/CIGP- VWOQTG s 2TQDGP 0KGTGPVWOQTG s 2TQDGP 1XCTKGPVWOQTG s 2TQDGP2TQUVCVCVWOQTGs2TQDGP Referenzdaten: #NU 4GHGTGP\FCVGP YWTFGP &CVGP FGU KPVGTPCVKQPCNGP

*CR/CR2TQLGMVU2JCUG4GNGCUG +PVGTPCVKQPCN *CR/CR 2TQLGEV

\W GKPGT 4GHGTGP\ \WUCOOGPIGHØJTV

*KGTDGK YWTFGP PWT UQNEJG 2TQDGP XGTYGPFGVFKGOKVFGOGenome-Wide Human SNP Array 6.0 CPCN[UKGTV YWT- FGP2TQDGP

Normalproben: <WUÀV\NKEJ \W FGP OCNKIPGP 2TKOÀTVWOQTRTQDGP YWT-

FGPPQEJ0QTOCNIGYGDGRTQDGP GDGPHCNNU XQP FGT )'1&CVGPDCPM UVCOOGPF 1XCTKGPIGYGDG s 2TQDGP )GJKTPIGYGDG s 2TQDGP .WPIGPIGYGDGs2TQDGP/CIGP- IGYGDGs2TQDGP0KGTGPIGYGDG sCWH$24UWPF5GIOGPVGXGTÀP- FGTVGT -QRKGP\CJN WPVGTUWEJV &KGUG

#PCN[UGP FKGPVGP \WT 8GTKȨ\KGTWPI FGT -TGDUURG\KȨVÀV RQVGP\KGNNGT IGPQ- OKUEJXGTÀPFGTVGT4GIKQPGPFGTOCNK- IPGP2TKOÀTVWOQTRTQDGP

#NIQTKVJOGP

&KG$GUVKOOWPIXQP5GIOGPVGPXGT- ÀPFGTVGT -QRKGP\CJN WPF VWOQTGPVK- VÀVUURG\KȨUEJGP MTGDUURG\KȨUEJGP WPF CNNIGOGKPGP $24U CPJCPF FGT 5025KIPCNKPVGPUKVÀVGPDGKPJCNVGVGFTGK YGUGPVNKEJG5EJTKVVG#NU'TUVGUGTHQNI- VGGKPG2TÀRTQ\GUUKGTWPIFGT502#T- TC[4QJFCVGPFCPPFKG7OTGEJPWPI FGT5KIPCNKPVGPUKVÀVGPHØTLGFGP502KP einen log₂4CVKQKP$G\WI\WT4GHGTGP\

WPFCDUEJNKG»GPFGKPG5GIOGPVKGTWPI .K 1NKXKGT -CTKORQWT(CTF GV CN &KG 2TÀRTQ\GUUKGTWPI GT- HQNIVGOKVFGT#HH[OGVTKZ2QYGT6QQNU

#265QHVYCTG#HH[OGVTKZ&KG YGKVGTG 8GTCTDGKVWPI FGT &CVGP YWT- de mit der freien Software R realisiert 4|XGTUKQP|4%QTG6GCO

&KG 5GIOGPVKGTWPI WPF CPUEJNKG»GP- FG %08 WPF $TWEJRWPMVTGIKQPU

$GUVKOOWPI GTHQNIVG HØT LGFG 6WOQT- RTQDGGKP\GNP&CDGKYWTFGHØTLGFGU 5GIOGPV FCU GPVURTGEJGPFG %JTQ- OQUQO FKG 5VCTV WPF 'PFRQUKVKQP FGU 5GIOGPVU FKG #P\CJN FGT 502U FKG FCU LGYGKNKIG 5GIOGPV WOHCUUV WPF FGT /KVVGNYGTV FGU LGYGKNKIGP 5GIOGPVU CPIGIGDGP &KG 5VCTV WPF 'PFRQUKVKQP GKPGU 5GIOGPVU UKPF IG- MGPP\GKEJPGV FWTEJ FKG IGPQOKUEJGP 2QUKVKQPGPFGUGTUVGPWPFNGV\VGP502U GKPGU 5GIOGPVU (ØT FKG $GUVKOOWPI FGTIGPQOKUEJGP2QUKVKQPGPFGT502U YWTFG FCU JWOCPG 4GHGTGP\IGPQO JI)4%JIGPWV\V#DD|

Erstellung der Referenz: <WT+FGPVKȨ-

\KGTWPIRQVGP\KGNNGTIGPQOKUEJGT8GT- ÀPFGTWPIGPYWTFGGKPG4GHGTGP\CWU

*CR/CR&CVGPIGPGTKGTV*KGTHØT YWTFGP FKG 4QJFCVGP \WPÀEJUV OKVVGNU #265QHVYCTG .KPWZ- XGTUKQP RTÀRTQ\GUUKGTV &KG YGKVGTG

&CVGPCPCN[UG GTHQNIVG OKV FGT 45QHV- YCTG&KG5KIPCNKPVGPUKVÀVGPHØTDGKFG

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#NNGNG YWTFGP \WUCOOGPIGHCUUV WPF HØT LGFGP 502 GKPG FWTEJUEJPKVVNKEJG 5KIPCNKPVGPUKVÀV ØDGT CNNG 2TQDGP DG- TGEJPGV YGNEJG CNU 4GHGTGP\ HØT FKG

#PCN[UGP FGT 6WOQTRTQDGP XGTYGP- FGVYWTFG

Präprozessierung und Bestimmung der Signalintensitäten: <WT +FGPVKȨ-

\KGTWPIXQP%08UWPF$24UDGKCNNGP OCNKIPGP 6WOQTRTQDGP XGT- UEJKGFGPGT 6WOQTGPVKVÀVGP GTHQNIVG

\WPÀEJUV FKG 2TÀRTQ\GUUKGTWPI FGT 4QJFCVGP OKVVGNU #26 *KGTDGK YWTFG HØT FKG 3WCPVKNPQTOCNKUKGTWPI WPF

*KPVGTITWPFMQTTGMVWT FGT $KTFUGGX

#NIQTKVJOWU WPVGT FGT 8GTYGPFWPI FGT 5VCPFCTFRCTCOGVGT CPIGYGPFGV

$GK FKGUGO JCPFGNV GU UKEJ WO GKPGP 5VCPFCTFCNIQTKVJOWU XQP #HH[OGVTKZ HØTFCU)GPQOG9KFG*WOCP502#T- TC[;CPIGVCN#PFTCFGGVCN

&KG2TÀRTQ\GUUKGTWPIFGTOCNK- IPGP 6WOQTRTQDGP YWTFG UGRCTCV XQPFGP0QTOCNRTQDGPFWTEJIG- HØJTV$GKFGPOCNKIPGP6WOQTRTQDGP GTHQNIVG FKG 2TÀRTQ\GUUKGTWPI LGYGKNU HØTCNNG2TQDGPGKPGT6WOQTGPVKVÀV+O

#PUEJNWUU CP FGP 2TÀRTQ\GUUKGTWPIU- UEJTKVV GTHQNIVG FKG #NNGN5WOOCVKQP WOHØTLGFGP502GKPG)GUCOVUKIPCN- KPVGPUKVÀV \W GTJCNVGP 0GWXKCN GV CN

Segmentierung: <WT &GVGMVKQP FGT IGPQOKUEJGP8GTÀPFGTWPIGPDGKOCNK- IPGP6WOQTGPYWTFGGKPG5GIOGPVKG- TWPILGFGT6WOQTRTQDGFWTEJIGHØJTV

*KGTHØT YWTFG \WPÀEJUV RTQ 6WOQT- RTQDG HØT LGFGP 502 FGT NQI₂-Ratio DGTGEJPGV&CDGKYWTFGFKGLGYGKNKIG )GUCOVUKIPCNKPVGPUKVÀV FGT 502U KPU 8GTJÀNVPKU\WFGTGPVURTGEJGPFGP4G- HGTGP\KPVGPUKVÀVFGU502UIGUGV\V.K 1NKXKGT .G 5EQWCTPGE )TKDDNG .G 5EQWCTPGE )TKDDNG

$CUKGTGPF CWH FKGUGP NQI4CVKQU GT- HQNIVG FCTCWHJKP FKG 5GIOGPVKGTWPI FGT#WVQUQOGPWOFCU)GPQOKP

\WUCOOGPJÀPIGPFG $GTGKEJG 5GI- OGPVG MQPUVCPVGT -QRKGP\CJN \W WP- VGTVGKNGP &KG 5GIOGPVKGTWPI GTHQNI- VG OKVVGNU FGU XQP $KQEQPFWEVQT \WT 8GTHØIWPI IGUVGNNVGP 42CMGVU &0#- EQR[ 8GTUKQP YGNEJGU FGP

%KTEWNCT$KPCT[5GIOGPVCVKQP%$5

#NIQTKVJOWU HØT FKG 5GIOGPVKGTWPI CPYGPFGV 5GUJCP 1NUJGP

*KGTDGKYWTFGFCU5KIPKȨMCP\NGXGNĮ CWH FKG 5VCPFCTFCDYGKEJWPI

dWPFQUFp CWH WPF FKG /KP- FGUVCP\CJNFGT/CTMGTdOKPYKFVJp FKGGKP5GIOGPVGPVJCNVGPOWUUCWH IGUGV\V8QTFGTLGYGKNKIGP5GIOGPVKG- TWPIGTHQNIVGKPG'TMGPPWPIFGT#WU- TGK»GT OKV GKPGT CPUEJNKG»GPFGP )NÀV- VWPIFGT&CVGPdUOQQVJ%0#p Bestimmung von genomischen Be- reichen veränderter Kopienzahl und Genen innerhalb dieser Regionen:

(ØT FKG +FGPVKȨ\KGTWPI IGPQOKUEJGT

$GTGKEJGXGTÀPFGTVGT-QRKGP\CJNYWT- FGPHØTLGFG6WOQTRTQDGCNNGGTOKVVGN- VGP 5GIOGPVG CPCN[UKGTV ,GFGT 502 2QUKVKQP YWTFG FGT GPVURTGEJGPFG 5GIOGPVOKVVGNYGTV \WIGQTFPGV WPF

ØDGT CNNG 2TQDGP GKPGT 6WOQTGPVKVÀV HØT LGFGP 502 GKP FWTEJUEJPKVVNKEJGT 5GIOGPVOKVVGNYGTVDGUVKOOV(ØTFKG- UG9GTVGYWTFGHØTLGFGU%JTQOQUQO GKPGT6WOQTGPVKVÀVUGRCTCVFGT/KVVGN- YGTVØDGTCNNG502UIGDKNFGVWOFCU )TWPFUKIPCN \W GTOKVVGNP #PUEJNKG-

»GPFYWTFGFCPPFKG&KHHGTGP\CWULG- FGOGKP\GNPGP5029GTVWPFFGOGPV- URTGEJGPFGP )TWPFUKIPCN DGUVKOOV

#PJCPF FKGUGT 9GTVG YWTFGP \WUCO- OGPJÀPIGPFG $GTGKEJG IGUWEJV FKG GKPG #DYGKEJWPI XQP FGT -QRKGP\CJN

\WFGT4GHGTGP\CWHYGKUGP*KGTDGKNKG- IGP CORNKȨ\KGTVG $GTGKEJG DGK GKPGO Wert ฺXQTWPFFGNGVKGTVG$GTGKEJG DGKGKPGO9GTVXQPู

Abb. 1) Schematische Darstellung des Aufbaus der entwickelten Pipeline und der untersuchten Proben. Zu- nächst wurden 2.820 Tumorproben, 990 HapMap-Proben der Phase 3, die als Referenz dienten, und 432 Normalproben, die als Vergleichsgruppe dienten, mit dem frei zugänglichen Tool APT präprozessiert. An- schließend wurden sowohl die log₂-Ratios aus Tumorproben und Referenzdaten als auch aus Normalproben und Referenzdaten gebildet und anschließend mittels CBS das Genom jeder Probe segmentiert. Anhand dieser 5GIOGPVKGTWPIYWTFGPFKGURG\KƌUEJGP$24UKFGPVKƌ\KGTV&KHHGTGP\5GIOGPVOKVVGNYGTVG| |'UYWTFGP FKGJQEJURG\KƌUEJGP$24U8QTMQOOGP KPOKPFGUVGPUGKPGT6WOQTGPVKVÀVWPFFKG\WUCOOGPJÀPIGP- FGP5GIOGPVGXGTUEJKGFGPGT-QRKGP\CJN&KHHGTGP\\WOEJTQOQUQOCNGP/KVVGNYGTV WPFFKGFCTKP MQFKGTVGP)GPGOKVVGNU$KQOC4VKFGPVKƌ\KGTV

REFERENZGRUPPE

*CR/CR2TQDGPFGT2JCUG Präprozessierung

mit APT

VERGLEICHSGRUPPE 0QTOCNRTQDGP Präprozessierung

mit APT

Berechnung log₂-Ratios

Segmentierung mittels CBS

+FGPVKȨ\KGTWPIURG\KȨUEJGT$24U

&KHHGTGP\5GIOGPVOKVVGNYGTVG

(KNVGTPXQPJQEJURG\KȨUEJGP$24U 8QTMQOOGP KPOKPFGUVGPU6WOQTGPVKVÀV

+FGPVKȨ\KGTWPIDGVTQHHGPGT)GPGWPF tumorassoziierter Gene

BiomaRt

+FGPVKȨ\KGTWPI5GIOGPVGXGTUEJKGFGPGT-QRKGP\CJN CORNKȨ\KGTV&KHHGTGP\

FGNGVKGTV&KHHGTGP\

Vergleich der BPRs:

6WOQTGPVKVÀVUURG\KȨUEJG$24U -TGDUURG\KȨUEJG$24U

#NNIGOGKPG$24U TUMORGRUPPE

6WOQTRTQDGP Präprozessierung

mit APT

9

THWildau

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+PPGTJCND FGT GTOKVVGNVGP 4GIKQPGP XGTÀPFGTVGT -QRKGP\CJN YWTFG PCEJ )GPGP IGUWEJV FKG FWTEJ GKPGP FG- NGVKGTVGP QFGT CORNKȨ\KGTVGP IGPQ- OKUEJGP $GTGKEJ DGGKPȩWUUV YGTFGP MÒPPGP&KGUG7PVGTUWEJWPIGTHQNIVG OKVVGNU$KQOC4V&WTKPEMGVCN

$KQOC4V KUV GKP 42CMGV YGNEJGU FGP

<WITKHHCWHXGTUEJKGFGPG&CVGPDCPMGP GTOÒINKEJV*KGTDGKYWTFGFKG)GPFG- VGMVKQPOKVVGNU'PUGODNTGCNKUKGTV Bestimmung der Bruchpunktregio- nen: <WT$GUVKOOWPIFGTEJTQOQUQ- OCNGP $24U GTHQNIVG HØT LGFG 6WOQT- RTQDGFKG$GVTCEJVWPICNNGTGTOKVVGNVGP 5GIOGPVG*KGTDGKYWTFGPPCEJGKPCP- FGT CNNG DGPCEJDCTVGP 5GIOGPVG FGU LGYGKNKIGP %JTQOQUQOU OKVGKPCPFGT XGTINKEJGP WPF GKPG RQVGP\KGNNG $24 FCPP CPIGPQOOGP YGPP FKG &KHHG- TGP\ FGT 5GIOGPVOKVVGNYGTVG \YGKGT DGPCEJDCTVGT5GIOGPVG DGVTWI FCUJGK»VFKGUG5GIOGPVGWPVGTUEJGK- FGPUKEJWOGKPG-QRKGP\CJN'KPGRQ- VGP\KGNNG$24GTIKDVUKEJFCPPCWUFGT IGPQOKUEJGP 'PFRQUKVKQP FGU GKPGP 5GIOGPVU WPF FGT 5VCTVRQUKVKQP FGU PCEJHQNIGPFGP 5GIOGPVU <YKUEJGP FKGUGPDGKFGP502UNKGIVGKPRQVGP\KGN- NGT$TWEJRWPMVFGTCWHITWPFFGU/KE- TQCTTC[.C[QWVU PKEJV GZCMV DGUVKOOV YGTFGP MCPP &CJGT MÒPPGP YKT PWT

$TWEJRWPMVTGIKQPGP WPF MGKPG GZ- CMVGP $TWEJRWPMVG DGUVKOOGP 7O CDUEJNKG»GPFURG\KȨUEJG$24UDGUVKO- OGP \W MÒPPGP GTHQNIVG FKG $GUVKO- OWPIFGT*ÀWȨIMGKVHØTFCU#WHVTGVGP LGFGTGTOKVVGNVGP$24ØDGTCNNG6WOQT- RTQDGP

'TIGDPKUUG

$TWEJRWPMVGCNNIGOGKP

/KV FGP DGUEJTKGDGPGP /GVJQFGP YWTFGPKPUIGUCOVØDGTCNNG|6W- OQTRTQDGP FGT | 6WOQTGPVKVÀVGP XGTUEJKGFGPG$TWEJRWPMVTGIKQ- PGP$24UKFGPVKȨ\KGTV&CXQPYWTFGP

|$24UIGHWPFGPFKGOKPFGUVGPU KP\YGKXGTUEJKGFGPGP6WOQTGPVKVÀVGP XQTMQOOGP&KGITÒ»VG$24GTUVTGEMV UKEJ ØDGT DR FKG MØT\GUVG ØDGT DR FWTEJUEJPKVVNKEJ WOHCUUV GKPG $24 DR 'U YWTFGP

$24U KFGPVKȨ\KGTV FKG KP ØDGT | CN- NGT 2TQDGP | $24U FKG KP ØDGT | FGT2TQDGPWPF|$24UFKGKPØDGT

| FGT 2TQDGP XQTMQOOGP +P FGP

WPVGTUWEJVGP 0QTOCNRTQDGP YWTFGP YGKVGTG | $24U KFGPVKȨ\KGTV FKG VGKNYGKUG\WUÀV\NKEJKPFGP6WOQTGP- VKVÀVGP XGTVTGVGP UKPF &CXQP MQO- OGP|$24UPWTKPFGP0QTOCN- RTQDGPXQT

$TWEJRWPMVXGTINGKEJ\YKUEJGP FGP6WOQTGPVKVÀVGP\WT/WUVGT GTMGPPWPI

7O URG\KȨUEJG /WUVGT \W KFGPVKȨ\KG- TGP FKG CWH GKPG -TGDUURG\KȨVÀV QFGT 6WOQTGPVKVÀVURG\KȨVÀV JKPFGWVGP YWTFGPHØTFKGLGYGKNKIGP$24UFKGTG- NCVKXGP*ÀWȨIMGKVGPRTQ6WOQTGPVKVÀV DGUVKOOVWPFOKVGKPCPFGTXGTINKEJGP 'UYWTFGP$24UKFGPVKȨ\KGTVFKGKP OKPFGUVGPU GKPGT 6WOQTGPVKVÀV ØDGT

|XQTMQOOGP&KGUG$24UYWTFGP CWHITWPFKJTGUJÀWȨIGP#WHVTGVGPUCNU JQEJURG\KȨUEJG $24U EJCTCMVGTKUKGTV +O (QNIGPFGP YKTF GKPG MNGKPG #WU- YCJN CP 'TIGDPKUUGP FKGUGT $GTGKEJG FCTIGUVGNNV/CTE\QMGVCN 'UMQPPVGP\YGK$24UKFGPVKȨ\KGTVYGT- FGPDGKFGPGPKPFGT6WOQTGPVKVÀ- VGPWPFKPFGP0QTOCNRTQDGPDKU\W

||UQYKGDKU\W||GKP

$TWEJFGT&0#KO%JTQOQUQODGK EC/DRCWHIGVTGVGPKUV

<WUÀV\NKEJ YWTFGP $24U FGVGMVKGTV FKG NGFKINKEJ KP GKPGT 6WOQTGPVKVÀV XGT- OGJTVCWHIGVTGVGPUKPF+O%JTQOQUQO

YWTFG KP )GJKTPVWOQTRTQDGP \W

|GKPG$24FGVGMVKGTV+PFGPTGUV- NKEJGP6WOQTGPVKVÀVGPVTCVFKGUGLGYGKNU KPYGPKIGTCNU|FGT2TQDGPCWH

#EJVFGTJQEJURG\KȨUEJGP$24UMQPP- VGP CWEJ KP FGP 0QTOCNRTQDGP OKV GKPGT *ÀWȨIMGKV XQP OGJT CNU | FGVGMVKGTVYGTFGP&KG*ÀWȨIMGKVFGU 8QTMQOOGPUKPFGP0QTOCNRTQDGPKUV CDGT KOOGT IGTKPIGT CNU OKPFGUVGPU GKPGFGT*ÀWȨIMGKVGPXQPFGP6WOQT GPVKVÀVGP

#WHITWPF FGT 'TIGDPKUUG FGT $24$G- UVKOOWPI MNCUUKȨ\KGTVGP YKT FKG $24U KPXKGTXGTUEJKGFGPG)TWRRGPCDJÀP- IKIXQPFGT*ÀWȨIMGKVFGU#WHVTGVGPU FGT$24WPFQDFKGUGCWEJKPFGP0QT- OCNRTQDGPIGHWPFGPYWTFG&KG'KP- VGKNWPIGTHQNIVGKP

VWOQTGPVKVÀVUURG\KȨUEJG $24U *ÀW- ȨIMGKVฺ | | CWUUEJNKG»NKEJ KP GKPGT 6WOQTGPVKVÀV6'QFGTฺ|KPGKPGT 6'WPFIGUWPFGO)GYGDGFG- VGMVKGTVG$24U

MTGDUURG\KȨUEJG $24U *ÀWȨIMGKV ฺ

|KPOGJTCNUGKPGT6'WPFKP0QT- OCNIGYGDG|FGVGMVKGTVG

$24UWPF

FKGCNNIGOGKPGP$24U*ÀWȨIMGKVฺ|

KPOGJTCNUGKPGT6'WPFฺ|0QT- OCNIGYGDGFGVGMVKGTVG$24U

Abb. 2) Bestimmung der strukturellen Veränderungen des Chromosoms 16 der Brusttumore. Es sind die Sig- PCNKPVGPUKVÀVGPNQI2-Ratios) pro genomischer Position jedes SNPs in Basenpaaren gegeneinander aufgetra- IGP&CU)TWPFUKIPCNNKGIVDGK'UKUVGKPGFGWVNKEJG#ORNKƌ\KGTWPIFGUR#TOGUWPF&GNGVKGTWPIFGU S#TOGUGTMGPPDCT<WUÀV\NKEJKUVKPPGTJCNDFGUS#TOGUGKPGURG\KƌUEJG#ORNKƌMCVKQPDGKDRDKU DRWPFGKPGURG\KƌUEJG&GNGVKQPDGKDRDKUDRYGNEJGUFCU)GP-+##

DRDKUDRWOUEJNKG»VGTMGPPDCT8QPDRDKUDRNKGIGPFKG zwei zugehörigen BPRs. Diese hatten über alle detektierten BPRs die höchste Vorkommenswahrscheinlichkeit aufgezeigt.

log25KIPCNKPVGPUKVÀV4GHGTGP\

IGPQOKUEJG2QUKVKQP=DR?

RTQVGKPMQFKGTGPFGU)GP KIAA0513

' '

' ' '

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&KG )TWRRG FGT PKEJV MTGDUURG\KȨ- UEJGP $24U DGKPJCNVGV LGPG YGNEJG OKV GKPGT *ÀWȨIMGKV XQP YGPKIGT CNU

| KP CNNGP 6WOQTGPVKVÀVGP FGVGMVKGTVG$24UFGVGMVKGTVYWTFGP 8GTINGKEJFGT$GTGKEJGXGTUEJKGFG-

PGT-QRKGP\CJNØDGTXGTUEJKGFGPG 6WOQTGPVKVÀVGP

7OFGPGXGPVWGNNGP'KPȩWUUFGT$24U CWH FKG 6WOQTGPVUVGJWPI WPF RTQ- ITGUUKQP \W CPCN[UKGTGP YWTFGP FKG )GPQODGTGKEJG WPVGTUWEJV FKG GKPG XGTÀPFGTVG-QRKGP\CJNCWHYGKUGPWO HGUV\WUVGNNGPQDKPFKGUGPDGUVKOOVG )GPG NQMCNKUKGTV UKPF 'U YWTFGP UQ- YQJNIGPQOKUEJG$GTGKEJGOKVXGTTKP- IGTVGT CNU CWEJ GTJÒJVGT -QRKGP\CJN KFGPVKȨ\KGTV

'U MQPPVGP KPUIGUCOV KP 0QTOCNRTQDGP \WUCOOGPJÀPIGPFG 5GIOGPVGOKVGKPGTXQPFGT4GHGTGP\

CDYGKEJGPFGP -QRKGP\CJN FGVGMVKGTV YGTFGP +PPGTJCND FKGUGT 5GIOGPVG NKGIGP KP 0QTOCNRTQDGP XGTUEJKGFGPG)GPGRTQVGKPMQFKGTGPF WPF PKEJV RTQVGKPMQFKGTGPF XQP FG- PGPDGKMGKPGKP0QTOCNRTQDGP DGMCPPVKUVFCUUUKGOKVFGT6WOQTIG- PGUGWPFRTQITGUUKQPKP8GTDKPFWPI UVGJGP

+P XQP 6WOQTGPVKVÀVGP MQPPVG KP

%JTQOQUQODGKEC/DRKO$G- reich des Genes KIAA0513 eine niedri- IGTG -QRKGP\CJN CNU KO IGPQOKUEJGP

/KVVGN FGVGMVKGTV YGTFGP <WFGO MQPPVGKPFKGUGO%JTQOQUQODGKEC /DR NGFKINKEJ KP $TWUVMTGDU GKPG RTÀIPCPVG #ORNKȨMCVKQP FGVGMVKGTV YGTFGP'TJÒJWPIWODKU\WXQO )TWPFUKIPCN#DD

<WUÀV\NKEJ KUV DGK )GJKTPVWOQTIGYG- DGP KO %JTQOQUQO FGT $GTGKEJ FGUR#TOUFGNGVKGTVWOIGTKPIGT CNUFCU)TWPFUKIPCN#DD'UMQPP- VG LGFQEJ CWEJ GKPG NGKEJVG &GNGVKQP

KP $TWUV -QNQTGMVCN WPF 1XCTKGPVW- OQTRTQDGP EC IGTKPIGT CNU FCU )TWPFUKIPCNHGUVIGUVGNNVYGTFGP&KG- UGT$GTGKEJWOUEJNKG»VFCU)GPTP53 +P %JTQOQUQO MQPPVG DGK EC /DR GKP $GTGKEJ OKV XGTTKPIGTVGT -Q- RKGP\CJNKP)GJKTPVWOQTGPWPVGT XQO )TWPFUKIPCN WPF GKPG YGPKIGT CWUIGRTÀIVGDGKEC/DRKFGPVKȨ\KGTV YGTFGP+PFKGUGO$GTGKEJKUVFCU)GP RP11-115C21.2MQFKGTV#DD

$GKFGP0QTOCNIGYGDGRTQDGPKUVFKG 8GTÀPFGTWPI FGT -QRKGP\CJN KOOGT geringer als mindestens eine der Ver- ÀPFGTWPIGPGKPGT6WOQTGPVKVÀV

&KUMWUUKQP

8GTINGKEJ KFGPVKȨ\KGTVGT $24U WPF 5GIOGPVG XGTÀPFGTVGT -QRKGP\CJN

\YKUEJGP 6WOQTRTQDGP WPF 0QT- OCNRTQDGP

&KG XQP WPU FWTEJIGHØJTVGP IGPQO- YGKVGP #PCN[UGP \GKIGP FCUU FKG

#P\CJN FGT KPUIGUCOV GTOKVVGNVGP

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(16)

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#7614'0

Birgit Bortz Stefanie Marczok

Prof. Dr. rer. nat. Heike Pospisil 6GEJPKUEJG*QEJUEJWNG9KNFCW (QTUEJWPIUITWRRG*KIJ2GTHQTOCPEG

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(18)

'NGMVTQIGURQPPGPG2QN[OGTHCUGTPCNUPGWCTVKIGU/CVGTKCN HØTFKG$KQGNGMVTQMCVCN[UGFGU'P\[OUPyrrolochinolin-

chinon-abhängige Glukosedehydrogenase

Johannes Gladisch, David Sarauli, Daniel Schäfer, Birgit Dietzel, Burkhard Schulz, Fred Lisdat*

Zusammenfassung

'U YWTFG GKP FTGKFKOGPUKQPCNGU 2QN[OGTHCUGTPGV\YGTM CWHIGDCWV EJCTCMVGTKUKGTV WPF CPUEJNKG»GPF FCTCP FCU 'P\[OPyrrolochinolinchinon-abhängige Glukosedehydro- genase 233)&* IGDWPFGP &CU 2QN[OGTHCUGTPGV\- YGTM YWTFG FWTEJ 'NGMVTQURKPPGP GKPGT /KUEJWPI FGU 2QN[OGTU 2QN[CET[NPKVTKN WPF XGTUEJKGFGPGT NGKVHÀJKIGT 2QN[OGTG FGT 2QN[CPKNKP(COKNKG CWH +PFKWO<KPP1ZKF 'NGMVTQFGP CWHIGDTCEJV &KG UQ JGTIGUVGNNVGP (CUGTOCV- VGP GTYKGUGP UKEJ DGK OKMTQUMQRKUEJGP 7PVGTUWEJWPIGP INGKEJHÒTOKI RTÀRCTKGTV WPF FKG (CUGTFWTEJOGUUGT DG- YGIVGPUKEJKO$GTGKEJYGPKIGTJWPFGTV0CPQOGVGT&CU 4GFQZRCCT-CNKWOJGZCE[CPQHGTTCV|+++++\GKIVGCPFKGUGP 2QN[OGT'NGMVTQFGPUVTWMVWTGP GKPG SWCUKTGXGTUKDNG 'NGM- VTQEJGOKG$GKYGKVGTIGJGPFGP7PVGTUWEJWPIGPCPFGP GP\[OOQFKȨ\KGTVGP (CUGTP 233)&* MQPPVGP WPVGT 5WDUVTCV\WICDG )NWMQUG DKQGNGMVTQMCVCN[VKUEJG 5VTÒOG PCEJIGYKGUGP YGTFGP &CU (CUGTPGV\YGTM HWPIKGTV JKGT PKEJVPWTCNU+OOQDKNKUKGTWPIUOCVTKZUQPFGTPCNUCWEJCNU 6GKNFGU5KIPCNYCPFNGTU

Abstract

#VJTGGFKOGPUKQPCNRQN[OGTKEGNGEVTQFGUVTWEVWTGYCUFG- XGNQRGFEJCTCEVGTK\GFCPFUWDUGSWGPVN[EQWRNGFYKVJVJG GP\[OGR[TTQNQSWKPQNKPGSWKPQPGFGRGPFGPV)NWEQUGFG- J[FTQIGPCUG 233)&* 6JG RQN[OGTKE ȨDGT PGVYQTM KU RTQFWEGF D[ OGCPU QH GNGEVTQURKPPKPI HTQO OKZVWTGU QH RQN[CET[NQPKVTKNG2#0CPFVJTGGFKHHGTGPVUWNHQPCVGFRQ[- NCPKNKPGU QP VQR QH +61 GNGEVTQFGU 6JG OCVU CTG WPKHQTO KPVJGKTQXGTCNNCRRGCTCPEGCXGTCIGFKCOGVGTUQHVJGȨDGTU RTQFWEGFCTGKPVJGTCPIGQHCHGYJWPFTGFPCPQOGVGTU 6JGUGRQN[OGTKEUVTWEVWTGUECPDGUJQYPVQCNNQYGNGEVTQ- EJGOKECN EQPXGTUKQPU CU XGTKȨGF YKVJ VJG HGTTKHGTTQE[C- PKFG TGFQZ EQWRNG +P CFFKVKQP CRRNKECVKQP KP DKQGNGEVTQ- ECVCN[UKU ECP DG FGOQPUVTCVGF (QT VYQ QH VJTGG UGNGEVGF DNGPFUQH2#0YKVJUWNHQPCVGFRQN[CPKNNKPGUCYGNNFGȨPGF DKQGNGEVTQEJGOKECN TGURQPUG KU QDVCKPGF WRQP EQXCNGPV ȨZCVKQPQH233)&*VQVJGȨDGTPGVYQTMCPFUWDUGSWGPV CFFKVKQPQHUWDUVTCVGINWEQUG6JGGNGEVTQURWPOCVTKZFQGU PQVQPN[CEVJGTGCUCPKOOQDKNK\CVKQPUWRRQTVDWVCVVJG UCOGVKOGCUCVTCPUFWEKPIGNGOGPV

WISSENSCHAFTLICHE BEITRÄGE 2016 ,CJTICPI

+550

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(19)

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+P FKGUGT #TDGKV YKTF FKG *GTUVGNNWPI FTGKFKOGPUKQPCNGTNGKVHÀJKIGT2QN[OGT- HCUGTPGV\YGTMG FWTEJ 'NGMVTQURKPPGP RTÀUGPVKGTV<WT*GTUVGNNWPIFGT(CUGTP YGTFGP)GOKUEJGFTGKGTWPVGTUEJKGF- NKEJ UWNHQPKGTVGT 2QN[CPKNKPG OKV PKEJV NGKV HÀJKIGO 2QN[CET [NPKVTKN GNGMVTQ- IGURQPPGP &CU 2#0 FKGPV FCDGK CNU UVTWMVWTIGDGPFGU 5VØV\RQN[OGT &KG (CUGTPGV\YGTMGYWTFGPCWH+61'NGM- VTQFGPCDIGUEJKGFGP&KGRTÀRCTKGTVGP

(CUGTPGV\YGTMGYWTFGPOKVVGNUOKMTQ- UMQRKUEJGT6GEJPKMGPUQYKGGNGMVTQEJG- OKUEJOKVFGOCPQTICPKUEJGP4GFQZRCCT -CNKWOJGZCE[CPQHGTTCV|+++++EJCTCM- VGTKUKGTV+PYGKVGTIGJGPFGP#PCN[UGP YWTFGFCU'P\[OPyrrolochinolinchinon- abhängige Glukosedehydrogenase233 )&*MQXCNGPVCPFKG(CUGTPIGDWPFGP WPFCPUEJNKG»GPFXQNVCOGVTKUEJWPVGT 5WDUVTCV\WICDG)NWMQUGWPVGTUWEJV

'TIGDPKUUGWPF&KUMWUUKQP

'UYWTFGPUWNHQPKGTVG2QN[CPKNKPGGKPIG- UGV\VYGKNFKGUGPKEJVPWTNGKVHÀJKIUKPF UQPFGTPCWEJGKPGFKTGMVG+PVGTCMVKQP OKV 233)&* GTNCWDGP 'U YWTFGP FTGKXGTUEJKGFGPG2QN[OGTGWPVGTUWEJV 2#$5#=RQN[COKPQDGP\QPUÀWTGEQ COKPQDGP\GPUWNHQPUÀWTG? 2#2#5#

=RQN[COKPQDGP\QNGUUKIUÀWTGEQCOK- PQDGP\GPGUWNHQPUÀWTG?WPF2#0#$/5#

=RQN[COKPQDGP\QPUÀWTGEQOGVJQ- Z[CPKNKPUWNHQPUÀWTGEQCPKNKP?#DD

#DIGUGJGPXQP5WNHQPUÀWTGITWRRGP YKGUGPFKG2QN[OGTGCWEJ%CTDQPUÀW- TGITWRRGPCNU5GKVGPMGVVGPCWH.GV\VGTG GTNCWDGPGKPGMQXCNGPVG#PMQRRNWPI FGU 'P\[OU 233)&* &KG 7PVGT- UEJKGFG FGT 2QN[OGTG GTIGDGP UKEJ FWTEJFKGXGTUEJKGFGPGP5WDUVKVWVKQPGP CO#PKNKPTKPI

Abb. 1) Chemische Struktur der verschiedenen XGTYGPFGVGP2QN[CPKNKPGC2#$5#D2#2#5#

E2#0#$/5#

*GTUVGNNWPIWPF%JCTCMVGTKUKGTWPI FGTFTGKFKOGPUKQPCNGP'NGMVTQFGP- UVTWMVWTGP

$GK GTUVGP 8GTUWEJGP OKV FGP TGKPGP 2QN[CPKNKPGP UVGNNVG UKEJ JGTCWU FCUU FKGUGCNNGKPPKEJVDGKO'NGMVTQURKPPGP\W XGTCTDGKVGPUKPF&KGUKUVYCJTUEJGKPNKEJ CWHFGTGPIGTKPIG8KUMQUKVÀVWPFIGTKPIG /QNOCUUG \WTØEM \WHØJTGP &CJGT YWTFGPFKGUWNHQPKGTVGP2QN[CPKNKPGKP 8GTDKPFWPIOKV2#0CNU5VØV\RQN[OGT XGTYGPFGV*KGTYWTFGP(CUGTPGTJCNVGP WPFCWHRNCPCTGP+61'NGMVTQFGPCDIG-

UEJKGFGP0CEJFGT*GTUVGNNWPIYWTFGP UKG \WPÀEJUV OKVVGNU .KEJVOKMTQUMQRKG WPVGTUWEJV WO FKG 3WCNKVÀV FGT (CUGT- PGV\YGTMG\WDGWTVGKNGP#PUEJNKG»GPF YWTFGP FKG 2QN[OGT HCUGTPGV\YGTMG OKVVGNU 4CUVGTGNGMVTQPGPOKMTQUMQRKG 4'/YGKVGTIGJGPFEJCTCMVGTKUKGTV

&KG#DD|WPF\GKIGP4'/#WHPCJOGP XQP2#02#25#2#02#0#$/5#WPF 2#02#2#5#2QN[OGTHCUGTPGV\YGTMGP

$GXQTFKG2QN[OGTOKUEJWPIGPKP&/51

\WO'NGMVTQURKPPGPGKPIGUGV\VYGTFGP MQPPVGP YWTFGP UKG OKV 7NVTCUEJCNN DGJCPFGNV WO GKPG JQOQIGPG 2QN[- OGTNÒUWPI\WGTJCNVGP$GKGKPGT2WOR- IGUEJYKPFKIMGKV XQP | NOKP YWTFG PCJG\WMGKPG6TQRHGPDKNFWPIHGUVIGUVGNNV CDGT\WXGTNÀUUKI(ÀFGPCDIGUEJKGFGP+P

#DJÀPIKIMGKV FGU IGYÀJNVGP 2QN[CPK- NKPU\GKIVGPFKG2QN[OGTHCUGTPGV\YGTMG WPVGTUEJKGFNKEJG (CTDGP 5Q YCTGP 2#02#$5# WPF 2#02#2#5# DTÀWP- NKEJYÀJTGPF2#02#0#$/5#DNÀWNKEJ YCT&KGHGUVIGUVGNNVGP(CTDGPUVKOOGP OKVFGPCPJCPFFGU4GFQZ\WUVCPFU\W GTYCTVGPFGP(CTDGPFGT2QN[OGTGPCEJ FGT5[PVJGUGØDGTGKP&KG2QN[OGTHCUGT- PGV\YGTMGWPVGTUEJKGFGPUKEJYGFGTDGK 8GTYGPFWPI XGTUEJKGFGPGT 2QN[OGT- IGOKUEJGPQEJDGKXGTUEJKGFGPGPOKV FGTUGNDGP2QN[OGTOKUEJWPIRTÀRCTKGTVGP 'NGMVTQFGPFGWVNKEJKPFGT(CUGTUVTWMVWT .GFKINKEJKPGKPKIGP(ÀNNGPKPUDGUQPFGTG HØT 2#02#0#$/5# MQPPVGP CIINQ- OGTKGTVG (CUGTP DGQDCEJVGV YGTFGP

&CUMCPPFWTEJFKGXGTINKEJGPOKVFGP CPFGTGP 2QN[OGTGP ITÒ»GTG 8KUMQ- UKVÀVXQP2#0#$/5#.ÒUWPIGPGTMNÀTV YGTFGP

&CUKP#DD|FCTIGUVGNNVG2QN[OGTHCUGT- PGV\YGTMYWTFGOKVGKPGT2TQ\GUUFCWGT XQP|OKPJGTIGUVGNNV&KG&KEMGFGU 2QN[OGTHCUGTPGV\YGTMUDGYGIVUKEJKO

$GTGKEJGKPKIGT/KMTQOGVGT&KGFWTEJ- UEJPKVVNKEJGP(CUGTFWTEJOGUUGTUKPFKP 6CD| \WUCOOGPIGHCUUV +O (CNN XQP 2#02#0#$/5# UKPF FKG (CUGTFWTEJ- OGUUGTWPFFGTGP8CTKCVKQPKO0GV\YGTM ITÒ»GTCNUKO(CNNFGTCPFGTGP2QN[OGTG

&CTØDGTJKPCWUVTCVGPDGK2#02#0#$/5#

FGWVNKEJITÒ»GTG8GTFKEMWPIGPCWHCNUDGK CPFGTGP2QN[OGTOKUEJWPIGP'UIKDVGKPG 8KGN\CJNOÒINKEJGT7TUCEJGPJKGTHØTGKP GPVUEJGKFGPFGT(CMVQTMÒPPVGCDGTFKG XGTJÀNVPKUOÀ»KIJQJG2TQ\GUUURCPPWPI XQP|M8UGKP*KPUKEJVNKEJFGT+PVGTCM- VKQPOKV9CUUGT\GKIVGPCNNG2QN[OGTHC- UGTPGV\YGTMGGKPÀJPNKEJJ[FTQRJKNGUWPF UEJYCOOCTVKIGU8GTJCNVGP-QPVCMVYKP- MGNOGUUWPIGPGTICDGPDGKCNNGP2QN[-

(20)

OGTGP9GTVGKO$GTGKEJXQP

'NGMVTQEJGOKUEJG%JCTCMVGTKUKGTWPI FGT2QN[OGTHCUGTPGV\YGTMG

/KV FGO <KGN FKG GNGMVTQEJGOKUEJGP 'KIGPUEJCHVGPFGTJGTIGUVGNNVGP2QN[- OGT HCUGTPGV \YGTMG \W DGUVKOOGP YWTFGP\[MNQXQNVCOGVTKUEJG7PVGTUW- EJWPIGPOKV-CNKWOJGZCE[CPQHGTTCV|++

+++FWTEJIGHØJTV&KGGNGMVTQEJGOKUEJGP 'KIGPUEJCHVGPYWTFGPOKVFGPGPXQP +61'NGMVTQFGP XGTINKEJGP FKG FKG

$CUKU HØT FKG 2QN[OGTHCUGTPGV\YGTMG FCTUVGNNVGP+O8GTINGKEJ\WDNCPMGP+61 'NGMVTQFGPMQPPVGFWTEJFCU#WHDTKPIGP FGT2QN[OGTHCUGTPGV\YGTMGGKPFGWVNKEJ XGTDGUUGTVGT 7OUCV\ XQP -CNKWOJG- ZCE[CPQHGTTCVHGUVIGUVGNNVYGTFGP+O 8GTINGKEJ \W FGP CPFGTGP 2QN[OGTGP

\GKIVG2#02#$5#FKGITÒ»VGP5VTQOOC- ZKOC#DD|6CD|2#02#2#5#WPF 2#02#$5#YGKUGPPKGFTKIGTG#DUVÀPFG

Abb. 2)4'/#WHPCJOGPFGT(CUGTOCVVGPXQPC+612#02#2#5#D+612#02#0#$/5#E+612#02#$5#.GIGPFG|||O

Abb. 3) 4'/#WHPCJOGPFGT(CUGTOCVVGPXQPC+612#02#2#5#D+612#02#0#$/5#E+612#02#$5#.GIGPFG|||O

Abb. 4) 4'/#WHPCJOGGKPGT+612#02#$5#/CVVG JGTIGUVGNNVFWTEJ|OKP'NGMVTQURKPPGP

Tab. 1:/KVVNGTG&WTEJOGUUGTFGT(CUGTPWPF(CUGTXGTFKEMWPIGPXGTUEJKGFGPGT (CUGTPGTOKVVGNVOKVVGNU'NGMVTQPGPOKMTQUMQRKGP

+612#0 2#2#5#

+612#0 2#0#$/5#

+612#0 2#$5#

&WTEJOGUUGT=O?

&WTEJOGUUGT8GTFKEMWPIGP=O?

NŞ#EO2 0 800 600

400