Phenotypic features and analysis of genes supporting probioticaction unravel underlying perspectives of Bacillus velezensis VTX9as a potential feed additive for swine

Supplementary Data

Phenotypic features and analysis of genes supporting probiotic action unravel underlying perspectives of Bacillus velezensis VTX9 as a potential feed additive for swine

Ngoc Tung Quach^1,2†, Thi Hanh Nguyen Vu^1,2†, Ngoc Anh Nguyen¹, Van The Nguyen¹, Thi Lien Bui¹, Son Chu Ky³, Tung Lam Le¹, Ha Hoang¹, Cao Cuong Ngo⁴, Thanh Thi Minh Le¹, Trung Nam Nguyen^1,2, Hoang Ha Chu^1,2, Quyet Tien Phi^1,2

1 Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 10000, Vietnam

2 Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 10000, Vietnam

3 School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi 100000, Vietnam

4 Vietnam-Russia Tropical Centre, Hanoi 100000, Vietnam

Correspondence:

Quyet Tien Phi, Associate Professor of Microbiology

Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam Postal Code: 10000

Phone No: +84-4-37917973 Fax No: +84-4-38363144 E-mail: tienpq@ibt.ac.vn

Contents:

Table S1

Table S2

Fig. S1

Supplementary Table and Figures

Table S1. Phenotypic characteristics and antibiotic sensitivity of Bacillus sp. VTX9 and the type strain B. velezensis CR-502^T.

Characteristic Bacillus sp. VTX9 B. velezensis CR-502^T

Morphological characteristics

Shape Rods Rods

Size 0.6–0.9×1.5–2.1μm 0.5 ×1.5-3.5 μm

Motility + +

Gram staining + +

Spore formation + +

Physiological properties

Temperature range for growth 25–45 ^oC 15–45 ^oC

Optimum temperature 37 ^oC ND

pH range for growth 2–9 5–10

Optimum pH 6 ND

NaCl range for growth 1–8% 1–12%

Optimum NaCl 4% ND

Biochemical properties

Catalase + +

Oxidase + +

Amylase + +

Protease + +

Cellulase + +

Carbon utilization

Mannitol - +

Glucose + +

Lactose + +

D-Fructose + +

Inositol + +

Melibiose - -

D-Raffinose + +

Amygdalin + +

D-Sorbitol + +

Methyl-α-D-glycoside + +

D-Turanose - -

Antibiotic sensitivity

Amikasin (30 µg) S ND

Nalidixic acid (30 µg) S S

Kanamycin (30 µg) S S

Neomicin (30 µg) S ND

Chloramphenicol (30 µg) S S

Gentamicin (10 µg) S ND

Ciprofloxacin (5 µg) S ND

Meropenem (10 µg) S ND

Ceftazidime (30 µg) S R

Fosfomycin (200 µg) S ND

Tetracycline (30 UI) S ND

Levofloxacin (5 µg) S ND

* (+), positive result; (-), negative result; ND, not determined; S, sensitive; R, resistant.

Table S2. Genomic features predicted in the chromosome and plasmids of B. velezensis VTX9

Features Chromosome

Plasmid

pVTX9-1 pVTX9-2 pVTX9-3 pVTX9-4 pVTX9-5

Genome size (bp) 3,985,800 20,007 8,565 18,221 12,395 7,261

G + C content (%) 46.1 37.6 39.9 35.3 38.1 42.2

Genes (total) 3,964 28 12 31 27 13

CDSs (coding) 3,736 28 12 31 27 13

rRNAs 27 0 0 0 0 0

tRNAs 86 0 0 0 0 0

ncRNAs 5 0 0 0 0 0

Pseudogenes 110 0 0 0 0 0

CRISPRS 0 0 0 0 0 0

GenBank accession number CP074685 CP074686 CP074687 CP074688 CP074689 CP074690

Fig. S1. Scanning electron microscopic images showing the cell morphology (a) and spore (b) of Bacillus sp. VTX9.

Bacillus sp. VTX9 was grown as an overnight culture in the EPS medium at 37 °C. Scale bar is shown in each figure.