Supporting online information for:
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Boron accumulation and toxicity in hybrid poplar (Populus nigra
× 2euramericana)
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RAINER REES1*, BRETT H. ROBINSON2, MANOJ MENON1, EBERHARD 5
LEHMANN3, MADELEINE S. GÜNTHARDT-GOERG4, RAINER SCHULIN1 6
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*corresponding author email: rainer.rees@env.ethz.ch, phone: +41 44 633 60 78 8
1 Institute of Terrestrial Ecosystems, ETH Zürich, Universitätsstrasse 16, 8092 9
Zürich, Switzerland 10
2 Soil and Physical Sciences, Burns 222, P O Box 84, Lincoln University, Lincoln 11
7647, Christchurch, New Zealand 12
3 Spallation Neutron Source Division, Paul-Scherrer-Institut, 5232 Villigen PSI, 13
Switzerland 14
4 Swiss Federal Institute for Forest, Snow and Landscape Research WSL, 15
Zürcherstrasse 111, 8903 Birmensdorf, Switzerland 16
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This SI document includes tables and figures with detail on the potting mix substrate 26
used in this study, a figure on the aboveground biomass production of the species 27
employed and two pictures of poplar leaves for the visualization of the pattern of 28
chlorosis and necrosis in the leaves.
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Figures & Tables 31
TABLE S1. Mean ± S. E. (N= 3) nitric-acid and CaCl2-extractable B concentration in 32
the control treatment T13 and the initially set-up B treatments T93, T168 and T280. 33
Boron concentration Treatment HNO3 CaCl2-
extractable
____________[mg kg-1]_____________
T13 13.01 ± 0.68 4.69 ± 0.43 T93 92.61 ± 6.92 27.70 ± 3.36 T168 167.5 ± 8.59 51.92 ± 4.50 T280 279.6 ± 2.35 133.3 ± 15.78 34
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TABLE S2. Mean ± S. E. concentrations of macro- and micro nutrients in the 43
unaltered PM substrate used as plants growth medium in this study. The 44
concentrations are valid for all treatments together.
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Concentration
Element HNO3 CaCl2-extractable
_______________[mg kg-1]________________
Ca 23125 ± 974.6 N/A
Fe 2724 ± 140.8 1.79 ± 0.38
K 2529 ± 236.0 1128 ± 145.2 Mg 1985 ± 108.4 454.9 ± 35.8
Mn 734.6 ± 32.2 37.52 ± 2.11 Na 471.1 ± 35.6 N/A
P 1792 ± 123.9 436.4 ± 131.9 Zn 141.8 ± 9.67 3.14 ± 0.30 N=12, N/A: not applicable
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FIGURE S1. Aboveground biomass production of P. nigra × euramericana, S.
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viminalis, L. albus and B. juncea during 4 months of growth on substrate with 54
different initial B concentrations. The lowest B concentration (13 mg kg-1) is the 55
control treatment. Error bars represent standard errors (N=3). N/A: not applicable. ƗƗƗƗ: : : : 56
plant died.
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FIGURE S2. Picture of a P. nigra × euramericana leaf from a plant grown on PM 60
substrate with a B concentration of 168 mg kg-1. Necrotic and chlorotic patches are 61
clearly visible at the leaf margins and within the leaves. Note that this is the same leaf 62
as shown on the right hand side in the TOC art.
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