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Sterols from a Vietnamese Wood-Rotting Phellinus sp. Dang Ngoc Quang

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Sterols from a Vietnamese Wood-Rotting Phellinus sp.

Dang Ngoc Quanga,b, Dang Dinh Bacha, and Yoshinori Asakawab

aFaculty of Chemistry, Hanoi University of Education, 136-Xuan Thuy Road, Cau Giay district, Hanoi, Vietnam

bFaculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan

Reprint requests to Dr. Dang Ngoc Quang. E-mail: quangdn@dhsphn.edu.vn Z. Naturforsch.2007,62b,289 – 292; received September 13, 2006

Phytochemical examination of the methanolic extract from fruit bodies of an unidentified Viet- namese Phellinusspecies led to the isolation of four compounds, one of which is a new steroid, 25-hydroxy-ergosta-7,24(28)-dien-3β-ol, named phellinol, together with senexonol, trametenolic acid B and ergosta-4,6,8(14),22-tetraen-3-one. Their structures were determined by 2D NMR, MS, IR and UV spectroscopy. In addition, the absolute configuration of senexonol was established by X-ray crystallographic analysis of its p-bromobenzoate derivative as 22(R)-hydroxy-4(S),14(S)- dimethyl-cholesta-8,24-dien-3-one. All compounds moderately suppressed the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in RAW 264.7 cells.

Key words: Phellinus, Sterol, Phellinol, Nitric Oxide

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