Biparental immune priming in the pipefish Syngnathus typhle
Table S5. Results from univariate statistical analysis of life history parameters and immune cell count measurements in one-week-old, four-month-old and six-month-old F1-juveniles. Univariate testing using ANOVAs for each response variable and the Tukey HSD post-hoc test was applied to test the effect of the two fixed factors F0-parents and F1- offspring, including their interaction effects. Corresponding levels of the fixed factor ‘F0- parents’: parental control (Control), paternal Vibrio (PatV+) and Tenacibaculum (PatT+), maternal Vibrio (MatV+) and Tenacibaculum (MatT+), biparental (Bi-(MatV+PatT+)/Bi- (MatT+PatV+)) treatment groups. Levels of the fixed factor ‘F1-offspring’: Vibrio (F1-V+), Tenacibaculum (F1-T+) and control (F1-N). Significant p-values are presented in boldface.
Response variables measured were body size of one-week- and four-month-old juveniles, for the latter also mass, hepatosomatic index (HIS), lymphocyte/monocyte ratio (L/M-ratio) of blood and head kidney (HK). Time post birth to reach sexual maturity was measured for approx. six-month-old F1-offspring.
ANOVA Factor Df F-value Pr(>F) TukeyHSD (Post-hoc) Residuals 378 One-week-old
Size
F0-parents 6 30.04 < 0.001
Control < MatT+; Control < MatV+; Control < PatV+;
Control < Bi-(MatT+PatV+); Control < Bi-(MatV+PatT+);
MatT+ < MatV+; MatT+ > PatT+; MatV+ > PatT+;
MatV+ > PatV+; PatT+ < Bi-(MatT+PatV+);
PatT+ < Bi-(MatV+PatT+); PatV+ < Bi-(MatV+PatT+);
MatV+ >Bi-(MatT+PatV+); MatV+ >Bi-(MatV+PatT+) Residuals 98 Four-month-old
Body Size
F0-parents 6 2.85 0.013 Control < MatV+
Body Mass
F0-parents 6 4.43 0.001 Control < Bi-(MatV+PatT+); Control < MatV+; PatT+ < MatV+;
PatT+ < Bi-(MatV+PatT+) HIS F0-parents 6 2.39 0.034 Control < PatT+
Residuals 73 Four-month-old L/M-ratio blood
F0-parents 6 5.15 <0.001 Control < PatV+
L/M-ratio HK
F0-parents 6 6.42 <0.001 Control < MatV+; Control < Bi(MatV+PatT+); Control < PatT+;
Control < PatV+; Control < Bi(MatT+PatV+); MatT+ < MatV+
L/M-ratio blood
F1-offspring 2 7.89 0.001 F1-N < F1V+
L/M-ratio HK F0-parents x
F1-offspring 12 2.98 0.002
ControlxF1N < PatV+xF1V+; ControlxF1N < MatV+xF1V+;
ControlxF1T+ < MatV+xF1V+; ControlxF1T+ < PatV+xF1V+;
ControlxF1V+ < PatV+xF1V+; Controlx F1V+ < MatV+x F1V+
MatT+xF1V+ < MatV+xF1V+; MatT+x F1V+ < MatV+x F1T+;
PatV+xF1T+ < MatV+xF1V+; MatT+xF1V+ < PatV+xF1V+;
PatV+xF1T+ < PatV+xF1V+
Residuals 168 Six-month-old Time to reach
sexual maturity F0-parents 3 0.001 Control < Mat; Control < Pat; Control < Bi; Pat < Bi; Mat < Bi
