Supplementary Material
A pathway map of signaling events triggered upon SARS-CoV infection
K.T. Shreya Parthasarathi1#, Nupur S. Munjal1#, Gourav Dey1, Abhishek Kumar1,2, Akhilesh Pandey3,4,5, Lavanya Balakrishnan6* and Jyoti Sharma 1,2*
1Institute of Bioinformatics, International Technology Park, Bangalore- 560066, India.
2Manipal Academy of Higher Education (MAHE), Manipal, Karnataka 576104, India.
3Center for Molecular Medicine, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bangalore 560 029, India.
4Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
5Center for Individualized Medicine, Mayo Clinic, Rochester, MN 55905, USA.
6Mazumdar Shaw Center for Translational Research, Narayana Hrudayalaya Health City, Bangalore, India.
# Equal contribution
*Corresponding Authors Dr. Lavanya Balakrishnan
Mazumdar Shaw Center for Translational Research, Narayana Hrudayalaya Health City, Bangalore, India; E-mail: lavanya.ibioinformatics@gmail.com
Dr. Jyoti Sharma
Institute of Bioinformatics, International Technology Park, Bangalore- 560066, India; E- mail:jyoti@ibioinformatics.org
S upplementary Figure 1: Depiction of reactions induced by Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) in SBGN format: The pathway reaction map depicts various types of reactions such as molecular associations, catalysis, and translocation events induced upon by SARS-CoV infection. Site and residue information of post-translational modifications are also provided as per available in literature. Important signal transduction modules including, PI3K-AKT, Ras-MAPK, JAK-STAT, Type 1 IFN and NFκB were found to be activated. The edges representing the relationships between nodes are provided in the legend.
Supplementary Table 1: List of cell lines used to study the biological functions induced upon SARS- CoV infection (Kaye 2006)
Cell line
Species of origin
Cell type Cytopathic effect (CPE)
Indirect immunofluorescence
assay (IDFA)
Quantitative PCR BGM African green
monkey, buffalo
Kidney epithelium
+ + +
COS Monkey Derivative of CV-1
- + +
CV-1 African green monkey
Kidney fibroblast
+ + +
FRhK Rhesus
monkey
Fetal kidney + + +
LLC- Mk2
Rhesus monkey
Kidney epithelium
+ + +
MA- 104
African green monkey
Kidney epithelium
+ + +
MEK Monkey Embryonic
kidney - + +
pCMK Monkey,
cynomolgus Primary kidney + + +
Vero African green monkey
Kidney epithelium
+ + +
Vero E6 African green monkey
Clone of Vero + + +
HEK- 293
Human Fetal kidney + + +
Hep G2 Human Liver
hepatocellular carcinoma
+ + +
Huh-7 Human Liver
hepatocellular carcinoma
+ + +
RK-13 Rabbit Kidney
epithelium
+ + +
Reference
Kaye M (2006) SARS-associated coronavirus replication in cell lines Emerg Infect Dis 12:128-133 doi:10.3201/eid1201.050496
