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Separation of Different Types of Monosaccharides by Polyacrylamide Column Chromatography

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Separation of Different Types of Monosaccharides by Polyacrylamide Column Chromatography

Slavom´ır Bystrick ´y, Izabela Medovarsk´a, and Eva Machov´a

Institute of Chemistry, GLYCOMED Centre, Slovak Academy of Sciences, 845 38 Bratislava, Slovakia

Reprint requests to Eva Machov´a. Phone: +421 2 5941 0221. E-mail: chememch@savba.sk Z. Naturforsch.2011,66b,295 – 298; received December 2, 2010

A high-throughput polyacrylamide gel chromatography was effectively tested to separate different types of monosaccharides. These occur for example in the complex bacterial LPS (lipopolysaccha- ride) structures as well as in other compounds. Here, a Bio-Gel P-2 column eluted only with water at ambient temperature afforded a convenient separation of the main structural types of neutral, charged, or highly polar hexoses, as well asN-acetylated hexose derivatives. These can be easily isolated on the semi-preparative scale by this method. The method was evaluated with a mixture of all basic types of monosaccharides, and repeatedly an efficient resolution was achieved. As an example of a complex structure, the monosaccharide constituents of the delipidated bacterial lipopolysaccharides (dLPS) ofV. choleraeO135 were effectively separated after acid hydrolysis.

Key words:Polyacrylamide Chromatography, Monosaccharides, Separation

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