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Compound α4β2 nAChR EC

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Compound α4β2 nAChR EC50(µM)

% activation (normalized to

Nicotine)

Nicotine 0.8 ± 0.1 100 %

Cotinine 85.3 ± 13.4 59 %

Anatabine 5.4 ± 1.1 80 %

Methylanatabine 6.2 ± 2.1 26 %

Anabasine 0.9 ± 0.2 7 %

Nornicotine 4.9 ± 0.7 44 %

Metanicotine 19.5 ± 6.4 100 % Acetylcholine 1.0 ± 1.0 100 %

AZD1446 7.9 ± 3.6 100 %

Online Resource 3 Summary of α4β2 nAChR EC50and percent activation

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